Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200506
Name   oriT_ICESsu05SC260 in_silico
Organism   Streptococcus suis integrative and
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   KX077888 (30692..30824 [-], 133 nt)
oriT length   133 nt
IRs (inverted repeats)      65..70, 83..88  (AAATCC..GGATTT)
 6..12, 23..29  (ACCCCCC..GGGGGGT)
Location of nic site      75..76
Conserved sequence flanking the
  nic site    		  TTTGGTTACA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 133 nt

>oriT_ICESsu05SC260
ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14555 GenBank   ANJ64473
Name   Relaxase_-_ICESsu05SC260 insolico UniProt ID   _
Length   621 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 621 a.a.        Molecular weight: 73794.64 Da        Isoelectric Point: 7.0198

>ANJ64473.1 relaxase [Streptococcus suis]
MVITKHYATHSRKYRKNLIRYILNPEKTAQLKLVSDFGMSNYLDFPSYEEMVKMYQANFLNNDGLYDSRN
DRQEKRQQKIHAHHLIQSFSPDDKLTPEEINRIGYETVKELTGGNFRFIVATHIDQSHIHNHILINSVDL
QSDKKLKWNYALERNLRMISDRISKEAGAKIITNRYSHQQYDVYRKTNHKFELKQRLYFLMEQSKDFEDF
LKKAEQLHLTIDFSSKHTTFLMTDRDQVKPIRGRQLNRREPYDESYFRQAFAKGAIEQRLHFLLPRVKTL
QELLEMAEELGLFIQLKQKNVTFTLTENDQKISLGHQQVSKKKLYDVSFFQDYFSERSDTDIELSDNIKE
DFEIFLAEQTEKLSTVESIVSDYETFSENRKAVHEFEVELAPHQVDKEVEGGIFIKVAFGVKKEGLIFIP
NYQLDEIEKEEEKRFKIFLREITSYFIYNKEGSDKNRYMKGRDLIRQLTQDNKSLPYRRRPNLKELQEKI
AEVNLLIELNVTNRAYTDIKDELVAEIATYDVSMSELNEKNATLNKMAEVLVNLKSHDIERRRLARYEFS
KLNLTESVTLEQIEREISNTQDQLSKLLDNYEESVRKLETFVAVLNRTAPRTRKDYDKELY

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(13-261)

Lantibiotic streptin immunity protein (Relaxase_C)

(517-608)


  Protein structure



No available structure.



ID   14556 GenBank   ANJ64491
Name   Rep_trans_-_ICESsu05SC260 insolico UniProt ID   _
Length   401 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 401 a.a.        Molecular weight: 47398.10 Da        Isoelectric Point: 6.5091

>ANJ64491.1 putative transcriptional regulator [Streptococcus suis]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

  Protein domains


Predicted by InterproScan.

Helix-turn-helix (HTH_3)

(15-53)

Replication initiation factor (Rep_trans)

(169-373)

Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N)

(73-161)


  Protein structure



No available structure.




Auxiliary protein

ID   7928 GenBank   ANJ64477
Name   ANJ64477_ICESsu05SC260 insolico UniProt ID   _
Length   405 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 405 a.a.        Molecular weight: 47021.92 Da        Isoelectric Point: 9.7978

>ANJ64477.1 integrase [Streptococcus suis]
MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKLVATDRVPAGKRDCISLREKIAELQKD
IHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSE
NGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYS
KNYDEILILLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQ
AFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFC
TNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA

  Protein domains


Predicted by InterproScan.

Phage integrase family (Phage_integrase)

(190-383)

DNA binding domain of tn916 integrase (Integrase_DNA)

(1-71)


  Protein structure



No available structure.




T4CP

ID   17172 GenBank   ANJ64493
Name   tcpA_-_ICESsu05SC260 insolico UniProt ID   _
Length   461 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 461 a.a.        Molecular weight: 53370.27 Da        Isoelectric Point: 9.0687

>ANJ64493.1 hypothetical protein [Streptococcus suis]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD

  Protein domains


Predicted by InterproScan.

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(217-301)

  Protein structure



No available structure.



ID   17173 GenBank   ANJ64541
Name   t4cp2_-_ICESsu05SC260 insolico UniProt ID   _
Length   605 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 605 a.a.        Molecular weight: 69397.18 Da        Isoelectric Point: 9.2929

>ANJ64541.1 hypothetical protein [Streptococcus suis]
MYSRRKAFVFGLLGLAFGYFCHRLTLLYDSLTNAPPMERFAYLLGEGLNQVFNPLWLFAFTQKSLLAFIL
GVLTMTLVYLYVSTGQKVYREGEEYGSARFGTSKEKRNFYSKNPFNDTILARDVRLTLLEKKKPLFDRNK
NLIVIGGSGAGKTFRFVKPNLIQLNCSNIVVDPKDHLAEKTGKLFLENGYQVKVLDLVNMTNSDGFNPFR
YVETENDLNRMLTVYFNNTRGSGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRGRYPA
FSEIGKLIKLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNI
QSVIDLTQRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALFFSTVFSTLTRQADVDFKGQLPIHVRSYL
DEFANVGEIPDFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNDEETFKFM
SGLLGKQTIDVRSTSRSFGQTGSSSTSHQKIARDLMTADEVGNMKRDECLVRIAGVPVFRTKKYFPLKHK
NWKWLADKETDERWWHYHINPLTAEEEVDLSGHKIRDLSTETTLH

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(135-555)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 22453..42589

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Locus_20 17641..17844 - 204 ANJ64478 Xis-Tn protein -
Locus_21 18113..18241 + 129 ANJ64479 hypothetical protein -
Locus_22 18305..18535 - 231 ANJ64480 hypothetical protein -
Locus_23 18532..18954 - 423 ANJ64481 hypothetical protein -
Locus_24 19458..19811 + 354 ANJ64482 hypothetical protein -
Locus_25 20157..22076 - 1920 ANJ64483 tetracycline resistance protein TetM -
Locus_26 22453..23385 - 933 ANJ64484 hypothetical protein orf13
Locus_27 23382..24383 - 1002 ANJ64485 NLP/P60 family lipoprotein orf14
Locus_28 24380..26491 - 2112 ANJ64486 putative membrane protein orf15
Locus_29 26560..29007 - 2448 ANJ64487 hypothetical protein virb4
Locus_30 28991..29383 - 393 ANJ64488 hypothetical protein orf17a
Locus_31 29472..29969 - 498 ANJ64489 antirestriction protein -
Locus_32 30086..30307 - 222 ANJ64490 conjugation related protein orf19
Locus_33 30350..31555 - 1206 ANJ64491 putative transcriptional regulator -
Locus_34 31578..31730 - 153 ANJ64492 hypothetical protein -
Locus_35 31733..33118 - 1386 ANJ64493 hypothetical protein virb4
Locus_36 33147..33530 - 384 ANJ64494 hypothetical protein orf23
Locus_37 33549..33863 - 315 ANJ64495 hypothetical protein orf23
Locus_38 33886..34005 - 120 ANJ64496 hypothetical protein -
Locus_39 34225..34524 + 300 ANJ64497 Abi-alpha protein -
Locus_40 34619..36436 - 1818 ANJ64498 UvrD/REP helicase family protein -
Locus_41 36423..38510 - 2088 ANJ64499 putative ATP-dependent endonuclease -
Locus_42 38507..39283 - 777 ANJ64500 zeta toxin -
Locus_43 39283..39759 - 477 ANJ64501 transcription regulator, probable -
Locus_44 39829..40119 - 291 ANJ64502 hypothetical protein -
Locus_45 40169..40558 - 390 ANJ64503 hypothetical protein gbs1346
Locus_46 40555..40782 - 228 ANJ64504 hypothetical protein gbs1347
Locus_47 40836..41912 - 1077 ANJ64505 hypothetical protein -
Locus_48 41951..42589 - 639 ANJ64506 hypothetical protein prgL
Locus_49 42588..42722 + 135 ANJ64507 hypothetical protein -
Locus_50 43990..44589 - 600 ANJ64508 DNA-binding response regulator -
Locus_51 44567..46075 - 1509 ANJ64509 putative two-component sensor histidine kinase -
Locus_52 46079..46768 - 690 ANJ64510 hypothetical protein -
Locus_53 46778..47497 - 720 ANJ64511 hypothetical protein -

Region 2: 61523..87059

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Locus_65 57265..57792 + 528 ANJ64523 adenine phosphoribosyltransferase -
Locus_66 58020..59339 + 1320 ANJ64524 transposase -
Locus_67 59528..60925 - 1398 ANJ64525 lantibiotic mersacidin modifying enzyme -
Locus_68 61009..61182 - 174 ANJ64526 hypothetical protein -
Locus_69 61523..61819 - 297 ANJ64527 hypothetical protein gbs1350
Locus_70 61833..62132 - 300 ANJ64528 hypothetical protein -
Locus_71 62203..69027 - 6825 ANJ64529 SNF2 family protein -
Locus_72 69077..69628 - 552 ANJ64530 hypothetical protein gbs1354
Locus_73 69612..69803 - 192 ANJ64531 putative calcium-binding protein -
Locus_74 69804..74699 - 4896 ANJ64532 agglutinin receptor prgB
Locus_75 74679..74834 + 156 ANJ64533 hypothetical protein -
Locus_76 74871..75461 + 591 ANJ64534 abortive infection protein AbiGI -
Locus_77 75458..76303 + 846 ANJ64535 abortive infection protein AbiGII -
Locus_78 76366..79167 - 2802 ANJ64536 hypothetical protein prgK
Locus_79 79169..81520 - 2352 ANJ64537 TrsE-like protein virb4
Locus_80 81477..81830 - 354 ANJ64538 hypothetical protein prgIc
Locus_81 81892..82746 - 855 ANJ64539 hypothetical protein prgHb
Locus_82 82765..83007 - 243 ANJ64540 hypothetical protein prgF
Locus_83 83025..84842 - 1818 ANJ64541 hypothetical protein virb4
Locus_84 84842..85330 - 489 ANJ64542 hypothetical protein gbs1365
Locus_85 85408..85992 - 585 ANJ64543 hypothetical protein -
Locus_86 85995..86228 - 234 ANJ64544 hypothetical protein -
Locus_87 86237..86620 - 384 ANJ64545 hypothetical protein -
Locus_88 86631..87059 - 429 ANJ64546 hypothetical protein gbs1369
Locus_89 87043..88398 - 1356 ANJ64547 modification methylase -
Locus_90 88547..89365 - 819 ANJ64548 hypothetical protein -
Locus_91 89362..89535 - 174 ANJ64549 hypothetical protein -
Locus_92 89595..89714 - 120 ANJ64550 hypothetical protein -
Locus_93 89734..90099 - 366 ANJ64551 LSU ribosomal protein L7/L12 (P1/P2) -

Host bacterium

ID   450 Element type   ICE (Integrative and conjugative element)
Element name   ICESsu05SC260 GenBank   KX077888
Element size   90099 bp Coordinate of oriT [Strand]   30692..30824 [-]
Host bacterium   Streptococcus suis integrative and Coordinate of element   875..89748

Cargo genes

Drug resistance gene   tet(M), ant(6)-Ia
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21, AcrIIA8