Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200505
• Name: oriT_ICESpnSPN8332
• Organism: Streptococcus pneumoniae integrative and
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): HG799498 (2166..2298 [+], 133 nt)
• oriT length: 133 nt
• IRs (inverted repeats): 65..70, 83..88 (AAATCC..GGATTT) 6..12, 23..29 (ACCCCCC..GGGGGGT)
• Location of nic site: 75..76
• Conserved sequence flanking the nic site: TTTGGTTACA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 133 nt

>oriT_ICESpnSPN8332
ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_-_ICESpnSPN8332

• ID: 14554
• GenBank: CDL74078
• Name: Rep_trans_-_ICESpnSPN8332
• UniProt ID: _
• Length: 501 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 501 a.a.
• Molecular weight: 59102.86 Da
• Isoelectric Point: 8.3800

>CDL74078.1 putative conjugative transposon replication initiation factor [Streptococcus pneumoniae]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKKGYLSTIPVDRYPKKDIMGD
KTVRVRADLHHIIKIETAKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRSGFKIGSVDTMLYANFENN
FEKAVFWYLRF

Protein domains

Predicted by InterproScan.

• Omega Transcriptional Repressor (Omega_Repress) (414-453)

• Helix-turn-helix (HTH_3) (15-53)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (73-161)

• Replication initiation factor (Rep_trans) (169-373)

Protein structure

No available structure.

Auxiliary protein

CDL74090_ICESpnSPN8332

• ID: 7927
• GenBank: CDL74090
• Name: CDL74090_ICESpnSPN8332
• UniProt ID: _
• Length: 405 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 405 a.a.
• Molecular weight: 46991.89 Da
• Isoelectric Point: 9.7978

>CDL74090.1 integrase [Streptococcus pneumoniae]
MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKLVATDRVPAGKRDCISLREKIAELQKD
IHDGIDVVGKKMALCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSE
NGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYS
KNYDEILILLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQ
AFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFC
TNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA

Protein domains

Predicted by InterproScan.

• DNA binding domain of tn916 integrase (Integrase_DNA) (1-71)

• Phage integrase family (Phage_integrase) (190-383)

Protein structure

No available structure.

T4CP

tcpA_-_ICESpnSPN8332

• ID: 17171
• GenBank: CDL74077
• Name: tcpA_-_ICESpnSPN8332
• UniProt ID: _
• Length: 461 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 461 a.a.
• Molecular weight: 53370.27 Da
• Isoelectric Point: 9.0687

>CDL74077.1 conjugative transposon FtsK/SpoIIIE-family protein [Streptococcus pneumoniae]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (217-301)

Protein structure

No available structure.

Host bacterium

• ID: 449
• Element type: ICE (Integrative and conjugative element)
• Element name: ICESpnSPN8332
• GenBank: HG799498
• Element size: 14353 bp
• Coordinate of oriT [Strand]: 2166..2298 [+]
• Host bacterium: Streptococcus pneumoniae integrative and
• Coordinate of element: 1..14353

Cargo genes

• Drug resistance gene: aph(3')-III, erm(B)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -