Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200497
• Name: oriT_ICESpn8140
• Organism: Streptococcus pneumoniae integrative and
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): FR671412 (15383..15529 [+], 147 nt)
• oriT length: 147 nt
• IRs (inverted repeats): 124..129, 133..138 (AAAGTG..CACTTT) 111..116, 124..129 (CACTTT..AAAGTG) 9..15, 28..34 (AACCCCC..GGGGGTT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 147 nt

>oriT_ICESpn8140
GAAACTTCAACCCCCGTTTTCTAATAGGGGGGTTACATTTGGCAAATACGCCAAGTCCACCCCTCCTCATTCCTTATGGGAGTTGGGTTTTCAATTTTTATGAAGTGTGTCACTTTCGTCAAAAAAGTGTGTCACTTTGTTAGAAAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_-_ICESpn8140

• ID: 14539
• GenBank: CBW39306
• Name: Rep_trans_-_ICESpn8140
• UniProt ID: _
• Length: 410 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 410 a.a.
• Molecular weight: 48328.83 Da
• Isoelectric Point: 5.7312

>CBW39306.1 Helix-turn-helix DNA binding protein [Streptococcus pneumoniae]
MDKISPFHIKNFRKQTGLSQKAFAQAVDLPTRTYRSYETGERGLTIDKFRELKEKLGYYQDCDKNSLRAQ
IDYLRLTFPRLKDLDAFCENFLHCHLSEFTDQETRLMNYTHLWQRGNIWIFDFFDKSVTNDYQTCLQLSG
QGCRELELLLEDKGITWQIFLQNILYSYEDVRVKRLDIALDELYKGYGHEDEQIQIPKLIDKLYSKEIVL
DTIKKWNITGGGSFTDNEDMEANHGLSIYFGSRQSQLYFNFYEKRYEIARMENISLDESLEIFGIWNRYE
LRFSDQKAQGIVEEYINGVDLGEIARGIINKEIQVYDGLTRFGAYKPDEKWQKLFGGVEPLKLSTNPQPY
SIERTIRWLTYQVANSLALVTEADKILQTEYMKMIQNSGEITDRGKAMLRLLKANKHYEH

Protein domains

Predicted by InterproScan.

• Helix-turn-helix (HTH_3) (9-57)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (69-162)

• Replication initiation factor (Rep_trans) (172-384)

Protein structure

No available structure.

T4CP

tcpA_-_ICESpn8140

• ID: 17158
• GenBank: CBW39305
• Name: tcpA_-_ICESpn8140
• UniProt ID: _
• Length: 562 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 562 a.a.
• Molecular weight: 65548.07 Da
• Isoelectric Point: 8.8833

>CBW39305.1 FtsK/SpoIIIE protein [Streptococcus pneumoniae]
MKLFTYRGKRVRVFHRKLKGITWFIWWFPFLLAISFYSYLHLEELKVHPLPQGYFLGAGLLFSLIFSWFI
NRVCYRKMLFFQKLNSLRILSRFLLENNLYLVKKVRRENKIIEKITLPQVYIKQSRYKIEVSFILEGNKF
QDRFLNLGATLEVMFNGDFRNKTFDNRFIKYEIAINRIDSRITIDEVKVKGSKLQLMKDVSWDYIEEPHL
LIGGGTGGGKTVVLMTIIYALAKIGFVDICDPKNSDLAGLKKIPVFHGRVYTSKEDIINCFKENVEFMEK
RYERMSTSPRFQAGKNFTHYDMKPKFILVDEWAALMAKIDRDYSQQSELMEYLSQLVLEGRQAGVFIIFA
MQRPDGEFIKTALRDNFMKRLSVGHLESTGYDMMFGDANKTKEFKKLDEINGVKVKGRGYIANNGELAGE
FFSPYVPLDQGFSFYDAYSKIPIMEFEGEEFSVFEEYKPPLETIDPIEEEETIENESNKRPLKEFADEQN
LKMPTLRKIIYLLTEQGIIFERTVSAILVDPFQEKLLLEILSQFEEGGRRSYPKAVEATIVHHGLGQGQG
QA

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (200-287)

Protein structure

No available structure.

Host bacterium

• ID: 442
• Element type: ICE (Integrative and conjugative element)
• Element name: ICESpn8140
• GenBank: FR671412
• Element size: 26888 bp
• Coordinate of oriT [Strand]: 15383..15529 [+]
• Host bacterium: Streptococcus pneumoniae integrative and
• Coordinate of element: 1..26888

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -