Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200491
• Name: oriT_ICE_SsaL64_fda
• Organism: Streptococcus salivarius integrative and
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): LT622834 (14440..14588 [+], 149 nt)
• oriT length: 149 nt
• IRs (inverted repeats): 111..116, 124..129 (CACTTT..AAAGTG) 102..107, 111..116 (AAAGTG..CACTTT) 9..15, 28..34 (AACCCCC..GGGGGTT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 149 nt

>oriT_ICE_SsaL64_fda
GAGACTTCAACCCCCGATTTCTAATAGGGGGGTTACATTTGGCCAAAGTGCCACGTCCACCCCTCCTCATTCCTTGTGGGAGTTGGGATTTCAGGATTTAGAAAGTGTGTCACTTTGGTCCAAAAAGTGTGTCACTTAGTCAAAAGGAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

ORFJ_-_ICE_SsaL64_fda

• ID: 14532
• GenBank: SCW20912
• Name: ORFJ_-_ICE_SsaL64_fda
• UniProt ID: _
• Length: 410 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 410 a.a.
• Molecular weight: 48256.72 Da
• Isoelectric Point: 6.1978

>SCW20912.1 Putative relaxase [Streptococcus salivarius]
MTKMSPFQIKNFRKQTGLSQKAFAQAVNLPIRTYRSYESGERGLTIDKFRELKEKLGYYQECHKNNLRAH
IDYLRLTFPSLRDLEAFCENFLFCHLSEFTDQETRLMNYTHLWQRGNIWIFDFFDKSATNNYQTCLQLSG
QGCREMELLLEHKGISWQTFLQNILYAYEDVRVKRLDIALDELYKGYGHEDEHIQIPKLIDKLYAKEIVL
DTIRKWNITGGGSFTDNEDMEANHGLSLYFGSRQSQLYFNFYEKRYEIARMENISLEESLEIFGIWNRYE
LRFSDQKAQGVVEEYINGVDLGEIARGIVNKEIQVYDGVTRFGAYKPDEKWQGLFGGVEPLKLSTSPQPY
SIERTIRWLTYQVANSLALVSEADKIMQTEYMKMIQNSGEITDRGEAILRLLKTNKHYEH

Protein domains

Predicted by InterproScan.

• Helix-turn-helix (HTH_3) (9-57)

• Replication initiation factor (Rep_trans) (172-384)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (69-162)

Protein structure

No available structure.

T4CP

ORFK_-_ICE_SsaL64_fda

• ID: 17148
• GenBank: SCW20911
• Name: ORFK_-_ICE_SsaL64_fda
• UniProt ID: _
• Length: 562 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 562 a.a.
• Molecular weight: 65096.27 Da
• Isoelectric Point: 7.2223

>SCW20911.1 FtsK/SpoIIIE family coupling protein [Streptococcus salivarius]
MKLFTYRGIRVRAFYRHIRGITWFIWWFPSLLAIGNYCYVNLEELRDHPLPLGYFIGGILFASCLVAYLL
NRMCYEKIVFFQKLNSLRILSRFLIENNLYLIKNIRRENKTIEKIVLPQVYLKQSRYKIEVHFILEGNKF
QDRFLNLGSTLEVMFNGDFRNKTFDKRFITYDIAINRINSRITINEVEVKGSKLQLMTDVTWDYIEEPHL
LIGGGTGGGKTVVLMTIIYALAKIGFVDICDPKNSDLAGLKKIPVFHGRVYTSKEDIIQCFKENVAFMEK
RYELMSNSPKFQAGKNFTHYGMTPKFILVDEWAALMAKIDRDYSLQSELMEYLSQLVLEGRQAGVFIIFA
MQRPDGEFIKTALRDNFMKRLSVGHLESTGYDMMFGDANKTKEFKKLDEINGVKVKGRGYIANNGDLAGE
FFSPYVPLDQGFSFYDAYAKIPIMEYSGEEFTVFREVRQQPETIEPIEEEKALNNETNQRPLKEFAEEQD
LKMATLRKIIYLLQEQGIVFERTESAILVDPFQEELLLESLIHFEEGGRKSYPKAVEATLSHHGLGQGQG
QA

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (200-287)

Protein structure

No available structure.

Host bacterium

• ID: 436
• Element type: ICE (Integrative and conjugative element)
• Element name: ICE_SsaL64_fda
• GenBank: LT622834
• Element size: 25797 bp
• Coordinate of oriT [Strand]: 14440..14588 [+]
• Host bacterium: Streptococcus salivarius integrative and
• Coordinate of element: 1..25797

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -