Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200489
• Name: oriT_ICE_SsaL50_rpsI
• Organism: Streptococcus salivarius integrative and
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): LT622832 (15751..15899 [+], 149 nt)
• oriT length: 149 nt
• IRs (inverted repeats): 111..116, 124..129 (CACTTT..AAAGTG) 102..107, 111..116 (AAAGTG..CACTTT) 9..15, 28..34 (AACCCCC..GGGGGTT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 149 nt

>oriT_ICE_SsaL50_rpsI
GAGACTTCAACCCCCGATTTCTAATAGGGGGGTTACATTTGGCCAAAGTGCCACGTCCACCCCTCCTCATTCCTTGTGGGAGTTGGGGTTTCAAGATTTAGAAAGTGTGTCACTTTGGTCCAAAAAGTGTGTCACTTAATCAAAAGGAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

ORFJ_-_ICE_SsaL50_rpsI

• ID: 14530
• GenBank: SCW20852
• Name: ORFJ_-_ICE_SsaL50_rpsI
• UniProt ID: _
• Length: 410 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 410 a.a.
• Molecular weight: 48370.93 Da
• Isoelectric Point: 6.5059

>SCW20852.1 Putative relaxase [Streptococcus salivarius]
MTKMSPFQIKNFRKQTGLSQKAFAQAVNLPIRTYRSYESGERGLTIDKFRELKEKLGYYQECHKNNLRAH
IDYLRLTFPCLRDLEAFCENFLFCHLSEFTDQETRLMNYTHLWQRGNIWIFDFFDKSATNNYQTCLQLSG
QGCREMELLLEHKGISWQTFLQNILYAYQDVRVKRLDIALDELYKGYGHEEEHIQIPKLIDKLYAKEIVL
DTIRKWNITGGGSFTDNDDMEANHGLSLYFGSRQSQLYFNFYEKRYEIARMENISLEESLEIFGIWNRYE
LRFSDQKAQGVVEEYINGVDLGEIARGIVNKEIQVYDGVTRFGAYKPDEKWQRLFGGVDPLKLSTSPQPY
SIERTIRWLTYQVANSLALVTEADKIMQTEYMKMIQNSGEITDRGEAILRLLKTNKHYEH

Protein domains

Predicted by InterproScan.

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (69-162)

• Helix-turn-helix (HTH_3) (9-57)

• Replication initiation factor (Rep_trans) (172-384)

Protein structure

No available structure.

T4CP

ORFK_-_ICE_SsaL50_rpsI

• ID: 17146
• GenBank: SCW20851
• Name: ORFK_-_ICE_SsaL50_rpsI
• UniProt ID: _
• Length: 562 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 562 a.a.
• Molecular weight: 65078.34 Da
• Isoelectric Point: 7.0121

>SCW20851.1 FtsK/SpoIIIE family coupling protein [Streptococcus salivarius]
MNLFTYRGIRVRAFYRHIRGITWFIWWFPSLLAIGHYCYLNLEELKDHPLPQVYFIGGVLVASCLVAYLL
NRMCYEKIVFFQKLNSLRILSRFLIENNLYLIKNIRRENKTIEKIVLPQVYLKQSRYKIEVRFILEGNKF
QDRFLNLGSTLEVMFNGDFRNKTFDKRFITYDIAINRIDSRITINEVEVKGSKLQLMKDVTWDYIEEPHL
LIGGGTGGGKTVVLMTIIYALAKIGFVDICDPKNSDLAGLKKIPVFHGRVYTSKEDIIQCFKENVTFMEK
RYELMSTSPKFQAGKNFTHYGMTPKFILVDEWAALMAKIDRDYSLQSELMEYLSQLVLEGRQAGVFIIFA
MQRPDGEFIKTALRDNFMKRLSVGHLESTGYDMMFGDANKTKEFKKLDEINGVKVKGRGYIANNGDLAGE
FFSPYVPLDQGFSFYDAYSKIPIMEFNGEEFEVFGEVRQQSKPIEPIEEEEALNTDTNLRPLKEFAEEED
LKMTTLRKIIYLLQKQGIVFERSDSAILVDPFQEELLLEILIHFEEGGRKSYPKAVEATLSHHGLGQGQG
QA

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (200-287)

Protein structure

No available structure.

Host bacterium

• ID: 434
• Element type: ICE (Integrative and conjugative element)
• Element name: ICE_SsaL50_rpsI
• GenBank: LT622832
• Element size: 27447 bp
• Coordinate of oriT [Strand]: 15751..15899 [+]
• Host bacterium: Streptococcus salivarius integrative and
• Coordinate of element: 1..27447

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -