Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200488
• Name: oriT_ICE_SsaL22_fda
• Organism: Streptococcus salivarius integrative and
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): LT622831 (24257..24405 [+], 149 nt)
• oriT length: 149 nt
• IRs (inverted repeats): 111..116, 124..129 (CACTTT..AAAGTG) 102..107, 111..116 (AAAGTG..CACTTT) 9..15, 28..34 (AACCCCC..GGGGGTT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 149 nt

>oriT_ICE_SsaL22_fda
GAGACTTCAACCCCCGATTTCTAATAGGGGGGTTACATTTGGCCAAAGTGCCACGTCCACCCCTCCTCATTCCTTGTGGGAGTTGGGATTTCAAGATTTAGAAAGTGTGTCACTTTGGTCCAAAAAGTGTGTCACTTAATTAAAAGGAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

ORFJ_-_ICE_SsaL22_fda

• ID: 14529
• GenBank: SCW20820
• Name: ORFJ_-_ICE_SsaL22_fda
• UniProt ID: _
• Length: 410 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 410 a.a.
• Molecular weight: 48325.92 Da
• Isoelectric Point: 6.6604

>SCW20820.1 Putative relaxase [Streptococcus salivarius]
MTKISPFQIKNFRKQTGLSQKDFAQAVNLPIRTYRSYESGERGLTIDKFRKLKEKLGYYQECHKNNLRAH
IDYLRLTFPCLRDLEAFCENFLFCHLSEFTDQETRLMNYTHLWQRGNIWIFDFFDKSATNNYQTCLQLSG
QGCREMELLLEHKGISWQTFLQTILYAYEDVRVKRLDIALDELYKGYGHEDEHIQIPKLIDKLYAKEIVL
DTIRKWNITGGGSFTDNDAMEANHGLSLYFGSRQSQLYFNFYEKRYEIARMENISLEESLEIFGIWNRYE
LRFSDQKAQGVVEEYINGVDLGEIARGIVNKEIQVYDGVTRFGAYKPDEKWQRLFGGVEPLKLSTSPQPY
SIERTIRWLTYQVANSLALVSEADKIMQTEYMKMIQNSGEITDRGEAILRLLKTNKHYEH

Protein domains

Predicted by InterproScan.

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (69-162)

• Helix-turn-helix (HTH_3) (9-57)

• Replication initiation factor (Rep_trans) (172-384)

Protein structure

No available structure.

T4CP

ORFK_-_ICE_SsaL22_fda

• ID: 17145
• GenBank: SCW20819
• Name: ORFK_-_ICE_SsaL22_fda
• UniProt ID: _
• Length: 562 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 562 a.a.
• Molecular weight: 64968.01 Da
• Isoelectric Point: 7.0122

>SCW20819.1 FtsK/SpoIIIE family coupling protein [Streptococcus salivarius]
MKLFTYRGIRVRAFYRHIRGITWFIWWFPSLLAIGNYCYVNLEELKDHPLPLGYFIGGVLFASCLVAYLL
NRMCYEKIVFFQKLNSLRILARFLIENNLYLIKNIRRENKTIEKIVLPQVYLKQSRYKIEVRFILEGNKF
QDRFLNLGSTLEVMFNGDFRNKTFDKRFITYDIAINRIDSRITINEVEVKGSKLQLMTDVTWDYIEEPHL
LIGGGTGGGKTVVLMTIIYALARIGFVDICDPKNSDLAGLKRIPVFHGRVYTSKEDIIQCFKENVAFMEK
RYELMSSSPKFQAGKNFTHYGMTPKFILVDEWAALMAKIDRDYSLQSELMEYLSQLVLEGRQAGVFIIFA
MQRPDGEFIKTALRDNFMKRLSVGHLESTGYDMMFGNANKTKEFKKLDEINGVKVKGRGYIANNGDLAGE
FFSPYVPLDQGFSFYDAYAKIPIMEFNGEEFEVFGEDHQQSEPIEAKEEEEAPNTKTNQRPLKEFAEEQD
LKMATLRKIIYLLQEQGIVFERTESAILVNPFQEELLLESLIHFEEGGRKSYPKAVEATLSHHGLGQGQG
QA

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (200-287)

Protein structure

No available structure.

Host bacterium

• ID: 433
• Element type: ICE (Integrative and conjugative element)
• Element name: ICE_SsaL22_fda
• GenBank: LT622831
• Element size: 37240 bp
• Coordinate of oriT [Strand]: 24257..24405 [+]
• Host bacterium: Streptococcus salivarius integrative and
• Coordinate of element: 1..37240

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -