Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200486
• Name: oriT_ICE_SsaF4-2_fda
• Organism: Streptococcus salivarius integrative and
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): LT622829 (18866..19014 [+], 149 nt)
• oriT length: 149 nt
• IRs (inverted repeats): 111..116, 124..129 (CACTTT..AAAGTG) 102..107, 111..116 (AAAGTG..CACTTT) 9..15, 28..34 (AACCCCC..GGGGGTT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 149 nt

>oriT_ICE_SsaF4-2_fda
GAGACTTCAACCCCCGATTTCTAATAGGGGGGTTACATTTGGCCAAAGTGCCACGTCCACCCCTCCTCATTCCTTGTGGGAGTTGGGATTTCAGGATTTAGAAAGTGTGTCACTTTAGTTCAAAAAGTGTGTCACTTAGTCAAAAGGAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

ORFJ_-_ICE_SsaF4-2_fda

• ID: 14527
• GenBank: SCW20749
• Name: ORFJ_-_ICE_SsaF4-2_fda
• UniProt ID: _
• Length: 410 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 410 a.a.
• Molecular weight: 48290.69 Da
• Isoelectric Point: 5.9926

>SCW20749.1 Putative relaxase [Streptococcus salivarius]
MTKMSPFQIKNFRKQTGLSQKAFAQAVNLPIRTYRSYESGERGLTIDKFRELKEKLGYYQECHKNNLRAH
IDYLRLTFPSLQDLEAFCENFLHCHLSEFTDQETRLMNYTHLWQRGNIWIFDFFDKSATNNYQTCLQLSG
QGCREMELLLEHKGISWQTFLQNILYGYEDVRVKRLDIALDELYKGYGHEEEHIQIPKLIDKLYAKEIVL
DTIRKWNITGGGSFTDNDDMEANHGLSLYFGSRQSQLYFNFYEKRYEIARMENISLEESLEIFGIWNRYE
LRFSDQKAQGVVEEYINGVDLGEIARGIVNKEIQVYDGITRFGAYKPDEKWQELFGGVEPLKLSTSPQPY
SIERTIRWLTYQVANSLALVSEADKIMQTEYMKMIQNSGEITDRGEAILRLLKTNKHYEH

Protein domains

Predicted by InterproScan.

• Helix-turn-helix (HTH_3) (9-57)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (69-162)

• Replication initiation factor (Rep_trans) (172-384)

Protein structure

No available structure.

T4CP

ORFK_-_ICE_SsaF4-2_fda

• ID: 17143
• GenBank: SCW20748
• Name: ORFK_-_ICE_SsaF4-2_fda
• UniProt ID: _
• Length: 562 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 562 a.a.
• Molecular weight: 64924.99 Da
• Isoelectric Point: 6.5131

>SCW20748.1 FtsK/SpoIIIE family coupling protein [Streptococcus salivarius]
MKLFTYRGIRVRAFYRHIRGITWFIWWFPSLLAIGHYCYLNLEELKDHPLPQVYFIGGVLVASCLVAYLL
NHMCYEKIVFFQKLNSLRILSRFLIENNLYLIKNIRRENKTIEKIDLPQVYLKQSRYKIEVCFILEGNKF
QDRFLNLGSTLEVMFNGDFRNKTFDKRFITYDIAINRIDSRISINEVEVKGSKLQLMKDVTWDYIEEPHL
LIGGGTGGGKTVVLMTIIYALAKIGFVDICDPKNSDLAGLKKIPVFHGRVYTSKEDIIQCFKENVAFMEK
RYELMSSSPKFQAGKNFTHYGMTPKFILVDEWAALMAKIDRDYSLQSELMEYLSQLVLEGRQAGVFIIFA
MQRPDGEFIKTALRDNFMKRLSVGHLESTGYDMMFGDANKTKEFKKLDEINGVKVKGRGYIANNGDLAGE
FFSPYVPLDQGFSFYEAYAKIPIMEFNGEEFEVFGEVHQQSKTIESIEEEEALSTETNQRPLKEFAEEQD
LKMATLRKIIYLLQEQGIVFERTESAILVDPFQEELLLESLIHFEEGGRKSYPKAVEATLSHHGLGQGQG
QA

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (200-287)

Protein structure

No available structure.

Host bacterium

• ID: 431
• Element type: ICE (Integrative and conjugative element)
• Element name: ICE_SsaF4-2_fda
• GenBank: LT622829
• Element size: 30222 bp
• Coordinate of oriT [Strand]: 18866..19014 [+]
• Host bacterium: Streptococcus salivarius integrative and
• Coordinate of element: 1..30222

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -