Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200482
• Name: oriT_ICE_SsaB35_rpsI
• Organism: Streptococcus salivarius integrative and
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): LT622825 (16712..16860 [+], 149 nt)
• oriT length: 149 nt
• IRs (inverted repeats): 111..116, 124..129 (CACTTT..AAAGTG) 102..107, 111..116 (AAAGTG..CACTTT) 9..15, 28..34 (AACCCCC..GGGGGTT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 149 nt

>oriT_ICE_SsaB35_rpsI
GAGACTTCAACCCCCGATTTCTAATAGGGGGGTTACATTTGGCAAAAGTGCCACGTCCACCCCTCCTCATTCCTTGTGGGAGTTGGGATTTCAGGATTTAGAAAGTGTGTCACTTTGGTCCAAAAAGTGTGTCACTTAATCAAAAGGAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

ORFJ_-_ICE_SsaB35_rpsI

• ID: 14523
• GenBank: SCW20628
• Name: ORFJ_-_ICE_SsaB35_rpsI
• UniProt ID: _
• Length: 410 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 410 a.a.
• Molecular weight: 48300.92 Da
• Isoelectric Point: 6.6603

>SCW20628.1 putative relaxase [Streptococcus salivarius]
MTKMSPFQIKNFRKQTGLSQKAFAQAVNLPIRTYRSYESGERGLTIDKFRKLKEKLGYYQECHKNNLRAH
IDYLRLTFPCLRDLEAFCENFLFCHLSEFTDQETRLMNYTHLWQRGNIWIFDFFDKSATNNYQTCLQLSG
QGCREMELLLEHKGISWQTFLQNILYAYQDVRVKRLDIALDELYKGYGHEEEHIQIPKLIDKLYAKEIVL
DTIKKWNITGGGSFTDNEDMEANHGLSLYFGSRQSQLYFNFYEKRYEIARMENISLEESLEIFGIWNRYE
LRFSDQKAQGVVEEYINGVDLGEIARGIVNKEIQVYDGVTRFGAYKPDEKWQSLFGGVEPLKLSTSPQPY
SIERTIRWLTYQVANSLALVTEADKIMQTEYMKMIQNSGEITDRGEAILRLLKTNKHYEH

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (172-384)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (69-162)

• Helix-turn-helix (HTH_3) (9-57)

Protein structure

No available structure.

T4CP

ORFK_-_ICE_SsaB35_rpsI

• ID: 17139
• GenBank: SCW20627
• Name: ORFK_-_ICE_SsaB35_rpsI
• UniProt ID: _
• Length: 561 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 561 a.a.
• Molecular weight: 65011.26 Da
• Isoelectric Point: 6.6688

>SCW20627.1 FtsK/SpoIIIE family coupling protein [Streptococcus salivarius]
MKLFTYRGIRVRAFYRHIRGITWFIWWFPSLLAIGHYCYLNLEELKEHPLPQVYYIGGILLASCLVAYLL
NRMCYEKIVFFQKLNSLRILSRFLIENNLYLIKNIRRENKTIEKIILPQVYLKQSRYKIEVRFILEGNKF
QDRFLNLGSTLEVMFNGDFRNKTFDKRFITYDIAINRIDSRITINEVEVKGFKLQLMTDVTWDYIEEPHL
LIGGGTGGGKTVVLMTIIYALAKIGFVDICDPKNSDLAGLKKIPVFHGRVYTSKEDIIQCFKENVAFMEK
RYELMSNSPKFQAGKNFTHYDMTPKFILVDEWAALMAKIDRDYSLQSELMEYLSQLVLEGRQAGVFIIFA
MQRPDGEFIKTALRDNFMKRLSVGHLESTGYDMMFGDANKTKEFKKLDEINGVKVKGRGYIANNGDLAGE
FFSPYVPLDQGFSFYDAYAKIPIMEYDGEEFEVFGEMSQQPEPIEPIKEEAPNTNTSLRPLKEFAEEQDL
KMATLRKIIYLLQEQGIVFERTESAILVDPFQEELLLESLIHFEEGGRKSYPKAVEATLSHHGLGQGQGQ
A

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (200-287)

Protein structure

No available structure.

Host bacterium

• ID: 427
• Element type: ICE (Integrative and conjugative element)
• Element name: ICE_SsaB35_rpsI
• GenBank: LT622825
• Element size: 28407 bp
• Coordinate of oriT [Strand]: 16712..16860 [+]
• Host bacterium: Streptococcus salivarius integrative and
• Coordinate of element: 1..28407

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -