Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200480 |
| Name | oriT_ICEBfrYCH46-1  |
| Organism | Bacteroides fragilis YCH46 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | AP006841 (18949..19145 [+], 197 nt) |
| oriT length | 197 nt |
| IRs (inverted repeats) | 10..17, 20..27 (TATGTTCA..TGAACATA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 197 nt
>oriT_ICEBfrYCH46-1
ACGGATTTTTATGTTCACCTGAACATAGCAAGATGTCTTTTCAGCCGCTCAAAATATTTTGAGCAGCCACGAAAAGCACTTGCCCTTGCAGGGGGCGGGCTTTCCCTCCGAAGTCGGGAAGCCTTTCAGAACTGCGGTGCTGTAATCCGAAGCGGCGGCTTATGCCGTCAATCCGCTAAATCCAAAGTAATATGAAA
ACGGATTTTTATGTTCACCTGAACATAGCAAGATGTCTTTTCAGCCGCTCAAAATATTTTGAGCAGCCACGAAAAGCACTTGCCCTTGCAGGGGGCGGGCTTTCCCTCCGAAGTCGGGAAGCCTTTCAGAACTGCGGTGCTGTAATCCGAAGCGGCGGCTTATGCCGTCAATCCGCTAAATCCAAAGTAATATGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14518 | GenBank | BAD46881 |
| Name | Relaxase_BF0132_ICEBfrYCH46-1 |
UniProt ID | _ |
| Length | 415 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 415 a.a. Molecular weight: 46563.08 Da Isoelectric Point: 9.8931
>BAD46881.1 conserved hypothetical protein [Bacteroides fragilis YCH46]
MVAKISVGSSLYGAIAYNGEKINEAQGRLLTTNRIYNDGSGTVDIGKAMEGFLTFLPPQMKIEKPVVHIS
LNPHPEDVLTDIELQNIAREYLEKLGFGNQPYLVFKHEDIDRHHLHIVTVNVDENGKRLNRDFLYRRSDR
IRRELEQKYGLHPAERKNQRLDNPLRKVAASAGDVKKQVGNTVKALNGQYRFQTMGEYRALLSLYNMTVE
EARGNVRGREYHGLVYSVTDDKGNKVGNPFKSSLFGKSAGYEAVQKKFVRSKSEIKDRKLADMTKRTVLS
VLQGTYDKDKFVSQLKEKGIDTVLRYTEEGRIYGATFIDHRTGCVLNGSRMGKELSANALQEHFTLPYAG
QPPIPLSIPVDAADKAHGQTAYDSEDISGGMGLLTPEGPAVDAEEEAFIRAMKRKKKKKRKGLGM
MVAKISVGSSLYGAIAYNGEKINEAQGRLLTTNRIYNDGSGTVDIGKAMEGFLTFLPPQMKIEKPVVHIS
LNPHPEDVLTDIELQNIAREYLEKLGFGNQPYLVFKHEDIDRHHLHIVTVNVDENGKRLNRDFLYRRSDR
IRRELEQKYGLHPAERKNQRLDNPLRKVAASAGDVKKQVGNTVKALNGQYRFQTMGEYRALLSLYNMTVE
EARGNVRGREYHGLVYSVTDDKGNKVGNPFKSSLFGKSAGYEAVQKKFVRSKSEIKDRKLADMTKRTVLS
VLQGTYDKDKFVSQLKEKGIDTVLRYTEEGRIYGATFIDHRTGCVLNGSRMGKELSANALQEHFTLPYAG
QPPIPLSIPVDAADKAHGQTAYDSEDISGGMGLLTPEGPAVDAEEEAFIRAMKRKKKKKRKGLGM
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 17134 | GenBank | BAD46882 |
| Name | t4cp2_BF0133_ICEBfrYCH46-1 |
UniProt ID | _ |
| Length | 672 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 672 a.a. Molecular weight: 77086.10 Da Isoelectric Point: 7.1001
>BAD46882.1 putative mobilization protein [Bacteroides fragilis YCH46]
MSQQEDDLRALAKIMDFLRAVSIILVVMNVYWFCYEAIRLWGVNIGVVDKILLNFDRTAGLFHSILYTKL
FSVLLLALSCLGTKGVKGEKITWGRIWTAFAVGFVLFFLNWWLLPLPLPLEAVTGLYVLTIGTGYVCLLM
GGLWMSRLLKHNLMEDVFNNENESFMQETRLIESEYSVNLPTRFYYRKRWNNGWINVVNPFRASIVLGTP
GSGKSYAVVNNFIKQQIEKGFSQYIYDFKYPDLSTIAYNHLLNHPDGYKVKPKFYVINFDDPRRSHRCNP
IHPDFMEDITDAYESAYTIMLNLNKTWVQKQGDFFVESPIILFASIIWYLKIYQNGKFCTFPHAIEFLNR
RYEDIFPILTSYPELENYLSPFMDAWLGGAAEQLMGQIASAKIPLSRMISPQLYWVMSDSEFTLDINNPE
EPKILCVGNNPDRQNIYGAALGLYNSRIVKLINKKGMLKSSVIIDELPTIYFKGLDNLIATARSNKVAVC
LGFQDFSQLVRDYGDKEAKVVMNTVGNIFSGQVVGETAKTLSERFGKVLQKRQSISINRQDVSTSINTQM
DALIPPSKISGLTQGMFVGSVSDNFNERIEQKIFHCEIVVDAEKVKREESAYKKIPVITNFTDEDGNDRM
KETVQANYRRIKEEVKQIVQEELERIKNDPVLCKLLPDNETV
MSQQEDDLRALAKIMDFLRAVSIILVVMNVYWFCYEAIRLWGVNIGVVDKILLNFDRTAGLFHSILYTKL
FSVLLLALSCLGTKGVKGEKITWGRIWTAFAVGFVLFFLNWWLLPLPLPLEAVTGLYVLTIGTGYVCLLM
GGLWMSRLLKHNLMEDVFNNENESFMQETRLIESEYSVNLPTRFYYRKRWNNGWINVVNPFRASIVLGTP
GSGKSYAVVNNFIKQQIEKGFSQYIYDFKYPDLSTIAYNHLLNHPDGYKVKPKFYVINFDDPRRSHRCNP
IHPDFMEDITDAYESAYTIMLNLNKTWVQKQGDFFVESPIILFASIIWYLKIYQNGKFCTFPHAIEFLNR
RYEDIFPILTSYPELENYLSPFMDAWLGGAAEQLMGQIASAKIPLSRMISPQLYWVMSDSEFTLDINNPE
EPKILCVGNNPDRQNIYGAALGLYNSRIVKLINKKGMLKSSVIIDELPTIYFKGLDNLIATARSNKVAVC
LGFQDFSQLVRDYGDKEAKVVMNTVGNIFSGQVVGETAKTLSERFGKVLQKRQSISINRQDVSTSINTQM
DALIPPSKISGLTQGMFVGSVSDNFNERIEQKIFHCEIVVDAEKVKREESAYKKIPVITNFTDEDGNDRM
KETVQANYRRIKEEVKQIVQEELERIKNDPVLCKLLPDNETV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 120020..130575
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BF0104 | 115091..115501 | + | 411 | BAD46853 | hypothetical protein | - |
| BF0105 | 115716..116111 | + | 396 | BAD46854 | conserved hypothetical protein | - |
| BF0106 | 116125..116541 | + | 417 | BAD46855 | hypothetical protein | - |
| BF0107 | 116538..117869 | + | 1332 | BAD46856 | hypothetical protein | - |
| BF0108 | 117894..118151 | + | 258 | BAD46857 | conserved hypothetical protein | - |
| BF0109 | 118320..118646 | + | 327 | BAD46858 | hypothetical protein | - |
| BF0110 | 118589..119008 | + | 420 | BAD46859 | hypothetical protein | - |
| BF0111 | 119042..119287 | - | 246 | BAD46860 | hypothetical protein | - |
| BF0112 | 119496..120020 | - | 525 | BAD46861 | probable lysozyme | - |
| BF0113 | 120020..120523 | - | 504 | BAD46862 | TraQ | traQ |
| BF0114 | 120520..121422 | - | 903 | BAD46863 | TraP | traP |
| BF0115 | 121430..122005 | - | 576 | BAD46864 | TraO | traO |
| BF0116 | 122008..122994 | - | 987 | BAD46865 | TraN | traN |
| BF0117 | 123029..124381 | - | 1353 | BAD46866 | TraM | traM |
| BF0118 | 124362..124670 | - | 309 | BAD46867 | TraL | traL |
| BF0119 | 124677..125300 | - | 624 | BAD46868 | TraK | traK |
| BF0120 | 125332..126336 | - | 1005 | BAD46869 | TraJ | traJ |
| BF0121 | 126340..126969 | - | 630 | BAD46870 | TraI | traI |
| BF0122 | 126993..127373 | - | 381 | BAD46871 | TraH | traH |
| BF0123 | 127414..129918 | - | 2505 | BAD46872 | TraG | virb4 |
| BF0124 | 129915..130247 | - | 333 | BAD46873 | TraF | traF |
| BF0125 | 130258..130575 | - | 318 | BAD46874 | TraE | traE |
| BF0126 | 130777..131487 | - | 711 | BAD46875 | TraD | - |
| BF0127 | 131522..131875 | - | 354 | BAD46876 | TraB | - |
| BF0128 | 131878..132318 | - | 441 | BAD46877 | conserved protein found in conjugate transposon | - |
| BF0129 | 132321..133370 | - | 1050 | BAD46878 | TraA | - |
| BF0130 | 133355..133522 | + | 168 | BAD46879 | hypothetical protein | - |
| BF0131 | 133792..134220 | + | 429 | BAD46880 | conserved hypothetical protein | - |
| BF0132 | 134199..135446 | + | 1248 | BAD46881 | conserved hypothetical protein | - |
Host bacterium
| ID | 425 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEBfrYCH46-1 | GenBank | AP006841 |
| Element size | 5277274 bp | Coordinate of oriT [Strand] | 18949..19145 [+] |
| Host bacterium | Bacteroides fragilis YCH46 | Coordinate of element | 114653..163822 |
Cargo genes
| Drug resistance gene | tet(Q) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA9 |