Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200478 |
| Name | oriT_ICETauDSM9187-1  |
| Organism | Tolumonas auensis DSM 9187 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP001616 (55844..56317 [-], 474 nt) |
| oriT length | 474 nt |
| IRs (inverted repeats) | 424..429, 439..444 (CGGGAA..TTCCCG) 153..159, 161..167 (GGGCTGC..GCAGCCC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 474 nt
>oriT_ICETauDSM9187-1
GAAGCGGGAAACTGCTGGACCAGCTCGGCGTAGTGCTCCAGCGGTGCGCGATACAAAGTGGCGAACTGTTTGCGCGATAGCGATGTGCGCTGCCAGATGTGTTCCAGCAGCTTCTGCCGGCGCGGCGTCGCCAGCAAGGACGCGGCCGGCTCGGGCTGCAGCAGCCCTTTGGGAAGATCGGCGGCGGGTGCCGGCGACGGAGCGGCAGTGACCGAGATCCGTTTTCGCTGGAACAGGGAGAGCATGCGAGTGTCCTGGTGATGGGCCGGCCGGGGGGCCTTTTCGCCTTTTCGAGGTAGGGCCTTTCCCCTTGCACCCCATTCCCTTCCCCTTTTGGCCCCTTGGCCCTTAAGCCTTTGGGTATAGGGCGACGGGCATCGATCGTCCACCGCCAATGCGGGGTGCGGCAAAGCCGGTTTGATGCGGGAAGGCTCGCTGTTCCCGTCCTACGATGGGCCGTATATTGGATTCGGA
GAAGCGGGAAACTGCTGGACCAGCTCGGCGTAGTGCTCCAGCGGTGCGCGATACAAAGTGGCGAACTGTTTGCGCGATAGCGATGTGCGCTGCCAGATGTGTTCCAGCAGCTTCTGCCGGCGCGGCGTCGCCAGCAAGGACGCGGCCGGCTCGGGCTGCAGCAGCCCTTTGGGAAGATCGGCGGCGGGTGCCGGCGACGGAGCGGCAGTGACCGAGATCCGTTTTCGCTGGAACAGGGAGAGCATGCGAGTGTCCTGGTGATGGGCCGGCCGGGGGGCCTTTTCGCCTTTTCGAGGTAGGGCCTTTCCCCTTGCACCCCATTCCCTTCCCCTTTTGGCCCCTTGGCCCTTAAGCCTTTGGGTATAGGGCGACGGGCATCGATCGTCCACCGCCAATGCGGGGTGCGGCAAAGCCGGTTTGATGCGGGAAGGCTCGCTGTTCCCGTCCTACGATGGGCCGTATATTGGATTCGGA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14515 | GenBank | ACQ94050 |
| Name | TraI_2_Tola_2455_ICETauDSM9187-1 |
UniProt ID | _ |
| Length | 618 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 618 a.a. Molecular weight: 67993.76 Da Isoelectric Point: 6.0564
>ACQ94050.1 Relaxase [Tolumonas auensis DSM 9187]
MLSLFQRKRISVTAAPSPAPAADLPKGLLQPEPAASLLATPRRQKLLEHIWQRTSLSRKQFATLYRAPLE
HYAELVQQFPASESHHHAYHGGMLDHGLEIIAYALKLRQSHLLPAGAPPEDQAAQSEAWTAAIAYAALLH
DIGKIAVDLHVEYEDGGVWHPWHGPLTRPYRFRYRDDREYRLHGAATGLLYHQLLDGDVLDWLSGYPALW
ASLLYVLAGQYEHAGILGELVIQADRASVAQELGGDPARAMAAPKHALQRKLIDGLRYLLKEELKLNQPQ
ASDGWLTEDALWLVSKTVSDKLRAHLLSQGIDGIPSNNSAVFNVLQEHGMLQPTPDGKAIWRATVSSEAG
WSHSFTLLRLSPALIWQPAERPEPFAGTVIVEAAAEADTLEQPATGTDDAPSSIRTEADSAHPVARQPPP
EATPADAAMDDLLSLLAVPAQESDGEREQPILDNSAPSIPPTVQPTDTDASPPKRAPEALGSTEPSGEHF
IAWLQQGIQARRLIINDAKALVHTVADTAYLVSPGLFQRYAQEHPSIAALAKQDKVQDWQWIQKRFEKLQ
LHRKQASGLNIWTCEVTGPRKSRRLHGYLLDDAGLLFSETPPNNPYLSLSDDTTNISL
MLSLFQRKRISVTAAPSPAPAADLPKGLLQPEPAASLLATPRRQKLLEHIWQRTSLSRKQFATLYRAPLE
HYAELVQQFPASESHHHAYHGGMLDHGLEIIAYALKLRQSHLLPAGAPPEDQAAQSEAWTAAIAYAALLH
DIGKIAVDLHVEYEDGGVWHPWHGPLTRPYRFRYRDDREYRLHGAATGLLYHQLLDGDVLDWLSGYPALW
ASLLYVLAGQYEHAGILGELVIQADRASVAQELGGDPARAMAAPKHALQRKLIDGLRYLLKEELKLNQPQ
ASDGWLTEDALWLVSKTVSDKLRAHLLSQGIDGIPSNNSAVFNVLQEHGMLQPTPDGKAIWRATVSSEAG
WSHSFTLLRLSPALIWQPAERPEPFAGTVIVEAAAEADTLEQPATGTDDAPSSIRTEADSAHPVARQPPP
EATPADAAMDDLLSLLAVPAQESDGEREQPILDNSAPSIPPTVQPTDTDASPPKRAPEALGSTEPSGEHF
IAWLQQGIQARRLIINDAKALVHTVADTAYLVSPGLFQRYAQEHPSIAALAKQDKVQDWQWIQKRFEKLQ
LHRKQASGLNIWTCEVTGPRKSRRLHGYLLDDAGLLFSETPPNNPYLSLSDDTTNISL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 17131 | GenBank | ACQ94028 |
| Name | t4cp2_Tola_2433_ICETauDSM9187-1 |
UniProt ID | _ |
| Length | 729 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 729 a.a. Molecular weight: 81331.19 Da Isoelectric Point: 6.7895
>ACQ94028.1 conserved hypothetical protein [Tolumonas auensis DSM 9187]
MSQTQPVEVLLRPAVELYTVAVCAGAAFLCLVAPWSLALSPAMGIGSALAFATFGAIRWRDAQVILRYRR
NIRRLPRYVMTSRDVPVSQQRLFIGKGFRWEQKHTHRLMQTYRPEFRRYVEPTPAYQLARRLEERLEFAP
FPLSRLSSLTSWDMPLNPVRPLPPVGGLPRLHGIEPEEVDVSLPLGERVGHSLVLGTTRVGKTRLAELFV
TQDIRRQNAAGEHEVVIVIDPKGDADLLKRVYVEAKRAGRENELYIFHLGWPDISARYNAVGRFGRISEV
ATRIAGQLSGEGNSAAFREFAWRFVNVIARALIELGQRPDYLLIQRHVINIDALFIEYAAHFFARTEPKA
WEVIVQIEAKLNEKNIPRNMIGREKRVVALEQYLSQARNYDPVLDGLRSAVRYDKTYFDKIVASLLPLLE
KLTSGKVAQLLAPNYSDLGDPRPIFDWMQVIRKRAIVYVGLDALSDAEVAAAVGNSMFSDLVSVAGHIYK
HGIDDGLPWASANTKVPINVHADEFNELMGDEFIPMINKGGGAGLQITAYTQTLSDIEARIGNRAKAGQV
VGNFNNVFMLRVRETATAELLTRQLPKVEVYTTTIVSGATDSSEIRGSTDFTSNTQDRISMSSVPMIEPS
HVVALPKGQCFALMQGGQLWKVRMPLPAADPDEAMPEDLQQLAGYMRQHYTEAAHWWENLGSPMLQDGPL
PDDLLDEAPIASDPPANVTGDTGLDEHTP
MSQTQPVEVLLRPAVELYTVAVCAGAAFLCLVAPWSLALSPAMGIGSALAFATFGAIRWRDAQVILRYRR
NIRRLPRYVMTSRDVPVSQQRLFIGKGFRWEQKHTHRLMQTYRPEFRRYVEPTPAYQLARRLEERLEFAP
FPLSRLSSLTSWDMPLNPVRPLPPVGGLPRLHGIEPEEVDVSLPLGERVGHSLVLGTTRVGKTRLAELFV
TQDIRRQNAAGEHEVVIVIDPKGDADLLKRVYVEAKRAGRENELYIFHLGWPDISARYNAVGRFGRISEV
ATRIAGQLSGEGNSAAFREFAWRFVNVIARALIELGQRPDYLLIQRHVINIDALFIEYAAHFFARTEPKA
WEVIVQIEAKLNEKNIPRNMIGREKRVVALEQYLSQARNYDPVLDGLRSAVRYDKTYFDKIVASLLPLLE
KLTSGKVAQLLAPNYSDLGDPRPIFDWMQVIRKRAIVYVGLDALSDAEVAAAVGNSMFSDLVSVAGHIYK
HGIDDGLPWASANTKVPINVHADEFNELMGDEFIPMINKGGGAGLQITAYTQTLSDIEARIGNRAKAGQV
VGNFNNVFMLRVRETATAELLTRQLPKVEVYTTTIVSGATDSSEIRGSTDFTSNTQDRISMSSVPMIEPS
HVVALPKGQCFALMQGGQLWKVRMPLPAADPDEAMPEDLQQLAGYMRQHYTEAAHWWENLGSPMLQDGPL
PDDLLDEAPIASDPPANVTGDTGLDEHTP
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2654915..2676431
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Tola_2425 | 2650255..2651031 | + | 777 | ACQ94020 | conserved hypothetical protein | - |
| Tola_2426 | 2651101..2652915 | - | 1815 | ACQ94021 | UvrD/REP helicase | - |
| Tola_2427 | 2652902..2654689 | - | 1788 | ACQ94022 | conserved hypothetical protein | - |
| Tola_2428 | 2654915..2655478 | + | 564 | ACQ94023 | conserved hypothetical protein | tfc2 |
| Tola_2429 | 2655475..2656134 | + | 660 | ACQ94024 | conserved hypothetical protein | - |
| Tola_2430 | 2656147..2656875 | + | 729 | ACQ94025 | putative secreted protein | tfc3 |
| Tola_2431 | 2656857..2657447 | + | 591 | ACQ94026 | Lytic transglycosylase catalytic | virB1 |
| Tola_2432 | 2657444..2657989 | + | 546 | ACQ94027 | putative secreted protein | tfc5 |
| Tola_2433 | 2657994..2660183 | + | 2190 | ACQ94028 | conserved hypothetical protein | - |
| Tola_2434 | 2660180..2660929 | + | 750 | ACQ94029 | conserved hypothetical protein | tfc7 |
| Tola_2435 | 2660930..2662300 | - | 1371 | ACQ94030 | conserved hypothetical protein | - |
| Tola_2436 | 2662464..2662841 | + | 378 | ACQ94031 | conserved hypothetical protein | tfc8 |
| Tola_2437 | 2662838..2663071 | + | 234 | ACQ94032 | conserved hypothetical protein | tfc9 |
| Tola_2438 | 2663088..2663450 | + | 363 | ACQ94033 | conserved hypothetical protein | tfc10 |
| Tola_2439 | 2663461..2663859 | + | 399 | ACQ94034 | conserved hypothetical protein | tfc11 |
| Tola_2440 | 2663856..2664536 | + | 681 | ACQ94035 | putative secreted protein | tfc12 |
| Tola_2441 | 2664533..2665435 | + | 903 | ACQ94036 | putative secreted protein | tfc13 |
| Tola_2442 | 2665425..2666834 | + | 1410 | ACQ94037 | putative secreted protein | tfc14 |
| Tola_2443 | 2666815..2667249 | + | 435 | ACQ94038 | putative secreted protein | tfc15 |
| Tola_2444 | 2667249..2670125 | + | 2877 | ACQ94039 | conserved hypothetical protein | virb4 |
| Tola_2445 | 2670141..2670899 | + | 759 | ACQ94040 | DsbA-thioredoxin family protein | - |
| Tola_2446 | 2671071..2671577 | + | 507 | ACQ94041 | DNA repair protein RadC | - |
| Tola_2447 | 2671713..2672213 | + | 501 | ACQ94042 | protein of unknown function DUF1525 | tfc24 |
| Tola_2448 | 2672210..2673142 | + | 933 | ACQ94043 | protein of unknown function DUF1527 | tfc23 |
| Tola_2449 | 2673153..2674550 | + | 1398 | ACQ94044 | conserved hypothetical protein | tfc22 |
| Tola_2450 | 2674547..2674897 | + | 351 | ACQ94045 | conserved hypothetical protein | tfc18 |
| Tola_2451 | 2674911..2676431 | + | 1521 | ACQ94046 | conserved hypothetical protein | tfc19 |
| Tola_2452 | 2676442..2676807 | - | 366 | ACQ94047 | conserved hypothetical protein | - |
| Tola_2453 | 2676835..2677293 | - | 459 | ACQ94048 | conserved hypothetical protein | - |
| Tola_2454 | 2677293..2677610 | - | 318 | ACQ94049 | transcriptional regulator, AbrB family | - |
| Tola_2455 | 2677917..2679773 | + | 1857 | ACQ94050 | Relaxase | - |
| Tola_2457 | 2680241..2681149 | - | 909 | ACQ94051 | carbamate kinase | - |
Host bacterium
| ID | 423 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICETauDSM9187-1 | GenBank | CP001616 |
| Element size | 3471292 bp | Coordinate of oriT [Strand] | 55844..56317 [-] |
| Host bacterium | Tolumonas auensis DSM 9187 | Coordinate of element | 2621845..2718871 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |