Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   200477
Name   oriT_ICEHaeULPAs1-1 in_silico
Organism   Herminiimonas arsenicoxydans
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   CU207211 (59314..59788 [-], 475 nt)
oriT length   475 nt
IRs (inverted repeats)      375..380, 393..398  (GCATTG..CAATGC)
 141..147, 154..160  (TGCGGCC..GGCCGCA)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 475 nt

>oriT_ICEHaeULPAs1-1
GAAGCGGGAAATTGCTGGACCAGCTCGGCATATCGTTCCAGCGGCGTGCGGTACAGGGTGACGAACTGCTTGCGCGAGAGGGAGGTGCGCTGCCAGATGTATTCCAGCAGCTTCTGCCGGCGCGGCGTGGCCAGCAGCGATGCGGCCGACTCGGGCCGCAGCAGCCCTTTCGGGAGATCGAGGGCGGGCGCTGGCGACGGAGCGGCAGCGACCGGAGGTCGTTTCCGCTGGAACAGAAAGAGCATGCGGGTGTCCTGGTGGTGGGCCGAGCGGGGGGCCTTTTCGCCTTTTCGAGGTAGGGCCTTTCCCCTTGCACCCCATTCCCTTGCCCTTTCGGCCCTTTAGCCTTTAACCATTTGGGTATAGGGCGTGGGGCATTGACCGTCCACGGCCAATGCGAGGTTCGGCGGGCCGGATTGATGCGTGAAGGCTTGGTGTTCCCGCCCTACGATGGGCCGATTCTTGGACTCGGGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   14514 GenBank   CAL62175
Name   TraI_2_HEAR2031_ICEHaeULPAs1-1 insolico UniProt ID   _
Length   614 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 614 a.a.        Molecular weight: 67517.78 Da        Isoelectric Point: 6.6258

>CAL62175.1 Conserved hypothetical protein, putative relaxase [Herminiimonas arsenicoxydans]
MLFLFQRKRPPVAAAPSPAPALDLPKGLLRPESAASLLATPRRQKLLEYIWQRTSLSRKQFVTLYRTPLE
RYAELVQQFPASESHHHAYPGGMLDHGLEIVAYSLKLRQSHLLPIGASPEDQAAQSEAWTAAVAYAALLH
DIGKIAVDLHVELADGNTWHPWHGPLLQPYRFRYREDREYRLHSAATGLLYRQLLDRHVLDWLSGYPALW
APLLYVLAGQYEHAGVLGELVVQADRASVAQELGGDPARVMAAPKHALQRKLLDGLRYLLKEELKLNQPE
ASDGWLTEDGLWLVSKTVSDKLRAHLLSQGIDGIPANNTAVFNVLQDHGMLQPTSDGKAVWRATVTSTTG
WSHSFTLLRLAPALIWESGERPAPFAGTVEIDATPAENDACMSAPAPTVSVNPAQGGQEPPIWEGDSTTI
VSPPAAQPVPDVMEDLLAMVGLGESAGVGQDAEEFLHTPAPATAATSIPSPAPAPAPTPGSSATKPSGEQ
FMVWLKQGIASRRLIINDAKALVHTVNETAYLVSPGVFQRYAQEHPEVAALAKQENQQDWQWVQKRFEKL
QLHRKQPNGLNIWTCEVTGPRKSRRLHGYLLENRSLVFAEIPPNNPYLALTQEG

  Protein domains


Predicted by InterproScan.

(27-340)

(487-609)


  Protein structure



No available structure.




T4CP


ID   17130 GenBank   CAL62149
Name   t4cp2_HEAR2005_ICEHaeULPAs1-1 insolico UniProt ID   _
Length   728 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 728 a.a.        Molecular weight: 80698.35 Da        Isoelectric Point: 7.3166

>CAL62149.1 Conserved hypothetical protein, putative ATPase domain [Herminiimonas arsenicoxydans]
MSGKQPVEVLLRPAVELYTVAACAGAAFLSLVAPWSLALSPAMGVGSALAFGAYGAIRYRDARVILCYRR
NIRRLPRYVMTSKDVPVSQQRLFVGRGFLWEQKHTHRLMQTYRPEFRRYVEPTPAYRLARRLEERLEFAP
FPLSRLSKLTGWDVPFNPVRPLPPVGGLPRLHGIEPDEVDVSLPLGERVGHSLVLGTTRVGKTRLAELFV
TQDIRRRNAAGEHEVVIVIDPKGDADLLKRMYVEAKRAGREGEFYVFHLGWPDISARYNAVGRFGRISEV
ATRIAGQLSGEGNSAAFREFAWRFVNIIARALVELGQRPDYMLIQRHVINIDALFIEYAQHYFAKTEPKA
WEVIVQIEAKLNEKNIPRNMIGREKRVVALEQYLSQARNYDPVLDGLRSAVRYDKTYFDKIVASLLPLLE
KLTSGKISQLLAPNYSDLADPRPIFDWMQVIRKRAVVYVGLDALSDAEVAAAVGNSMFSDLVSVAGHIYK
HGIDDGLPGASAGTRVPINVHADEFNELMGDEFVPLINKGGGAGLQVTAYTQTLSDIEARIGNRAKAGQV
IGNFNNLFMLRVRETATAELLTRQLPKVEVYTTTIVSGATDSSDIRGATDFTSNTQDRISMSSVPMIEPS
HVVALPKGQCFALLQGGQLWKVRMPLPAPDPDEVMPQDLQQLAGYMRQSYSEATQWWEFTSSPALQDGAL
PDDLLDDAAAAEPGPVATDDSAGNEASP

  Protein domains


Predicted by InterproScan.

(508-636)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 2010192..2036064

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HEAR1997 2005559..2006338 + 780 CAL62141 Conserved hypothetical protein, putative competence protein -
HEAR1998 2006405..2008171 - 1767 CAL62142 putative DNA helicase II -
HEAR1999 2008206..2009990 - 1785 CAL62143 Conserved hypothetical protein, putative ATP-dependent endonuclease -
HEAR2000 2010192..2010812 + 621 CAL62144 Conserved hypothetical protein tfc2
HEAR2001 2010809..2011459 + 651 CAL62145 Conserved hypothetical protein -
HEAR2002 2011474..2012199 + 726 CAL62146 Conserved hypothetical protein; putative exported protein tfc3
HEAR2003 2012181..2012771 + 591 CAL62147 Conserved hypothetical protein, putative lytic transglycolase virB1
HEAR2004 2012768..2013316 + 549 CAL62148 Conserved hypothetical protein; putative exported protein tfc5
HEAR2005 2013321..2015507 + 2187 CAL62149 Conserved hypothetical protein, putative ATPase domain -
HEAR2006 2015504..2016253 + 750 CAL62150 Conserved hypothetical protein; putative membrane protein tfc7
HEAR2007 2016283..2018907 - 2625 CAL62151 Conserved hypothetical protein, putative ATPase involved in DNA repair -
HEAR2008 2019008..2020060 - 1053 CAL62152 Conserved hypothetical protein -
HEAR2010 2020212..2020886 - 675 CAL62154 Conserved hypothetical protein -
HEAR2011 2020949..2021893 + 945 CAL62155 Conserved hypothetical protein -
HEAR2012 2022030..2022410 + 381 CAL62156 Conserved hypothetical protein; putative exported protein tfc8
HEAR2013 2022407..2022640 + 234 CAL62157 Conserved hypothetical protein; putative membrane protein tfc9
HEAR2014 2022657..2023016 + 360 CAL62158 Conserved hypothetical protein; putative exported or membrane protein tfc10
HEAR2015 2023029..2023439 + 411 CAL62159 Conserved hypothetical protein; putative membrane protein tfc11
HEAR2016 2023436..2024125 + 690 CAL62160 Conserved hypothetical protein tfc12
HEAR2017 2024122..2025039 + 918 CAL62161 Conserved hypothetical protein; putative exported protein tfc13
HEAR2018 2025029..2026459 + 1431 CAL62162 Conserved hypothetical protein tfc14
HEAR2019 2026440..2026880 + 441 CAL62163 Conserved hypothetical protein; putative exported protein tfc15
HEAR2020 2026880..2029762 + 2883 CAL62164 Conserved hypothetical protein virb4
HEAR2021 2029776..2030540 + 765 CAL62165 Conserved hypothetical protein, putative disulfide isomerase -
HEAR2022 2030725..2031219 + 495 CAL62166 putative DNA repair protein RadC -
HEAR2023 2031378..2031824 + 447 CAL62167 Conserved hypothetical protein tfc24
HEAR2024 2031821..2032768 + 948 CAL62168 Conserved hypothetical protein tfc23
HEAR2025 2032778..2034175 + 1398 CAL62169 Conserved hypothetical protein; putative exported protein tfc22
HEAR2026 2034172..2034531 + 360 CAL62170 Conserved hypothetical protein; putative exported protein tfc18
HEAR2027 2034547..2036064 + 1518 CAL62171 Conserved hypothetical protein; putative membrane protein tfc19
HEAR2028 2036078..2036455 - 378 CAL62172 Conserved hypothetical protein; putative exported protein -
HEAR2029 2036483..2036941 - 459 CAL62173 Conserved hypothetical protein -
HEAR2030 2036941..2037258 - 318 CAL62174 Conserved hypothetical protein, putative transcriptional regulator -
HEAR2031 2037564..2039408 + 1845 CAL62175 Conserved hypothetical protein, putative relaxase -


Host bacterium


ID   422 Element type   ICE (Integrative and conjugative element)
Element name   ICEHaeULPAs1-1 GenBank   CU207211
Element size   3424307 bp Coordinate of oriT [Strand]   59314..59788 [-]
Host bacterium   Herminiimonas arsenicoxydans Coordinate of element   1978021..2066138

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   arsH, arsC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -