Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   200476
Name   oriT_ICEXcaVe85-1 in_silico
Organism   Xanthomonas campestris pv. vesicatoria
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   AM039952 (101397..101714 [+], 318 nt)
oriT length   318 nt
IRs (inverted repeats)      197..202, 207..212  (TTTAAA..TTTAAA)
 188..194, 199..205  (CCCTTTA..TAAAGGG)
 34..39, 41..46  (GGAACG..CGTTCC)
Location of nic site      19..20
Conserved sequence flanking the
  nic site  
 
 TCGTGCCTGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 318 nt

>oriT_ICEXcaVe85-1
CCATGGCCGAGCGGTCGTGCCTGCGCCAGCGAAGGAACGTCGTTCCCGCGCCAGTTGCCTGCTGGGCCAGCTCCACGGGCAGCAGATGGAAGGGATGCCCGGCTGCCTGTTGCAGCACCTGTCGCTGCGCCAAGCCAATCAATTCGTCGCGCATGGCGAAGCACTGGCTGGCCCAGGCCTCCAAGTCCCCTTTACCTTTAAAGGGTTTTAAAAGGCCTTTTTGAGAGCCTGCGTGTTCCAGCCTCATGAAGGCGGTCTGTTGCAGGGGACGGAAGTAGCGGGTTTCGCGATTCGGGTCGCTCATGCCTGCTCGTCCTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   14513 GenBank   CAJ24033
Name   TraI_2_XCV2356_ICEXcaVe85-1 insolico UniProt ID   _
Length   610 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 610 a.a.        Molecular weight: 66988.24 Da        Isoelectric Point: 6.8482

>CAJ24033.1 conserved hypothetical protein [Xanthomonas euvesicatoria pv. vesicatoria str. 85-10]
MLSLFQRKRPPVAAAPSPPPAIDLPKGLMRPESAASLLATPRRQKLLEHIWQRTSLSRRQFATLYRAPLE
RYAELVQGFPASEAHHHAYLGGMLDHGLEIVAYGLKLRQSHLLPVGASPEDQAAQAEAWTAAVAYAALLH
DIGKVAVDLHVELADGSTWHPWHGPLRQPYRFRYRDDREYRLHSAATGLLYRQLLDRDLLDWLSGYPSLW
APLLYVLAGQYEHAGVLGELVVQADRASVAQELGGDPARAMAAPKHSLQRKLVNGLRYLLKEELKLNQPE
ASDGWLTEDALWLVSKTVSDKLRAHLLSQGVDGIPANNTAVFNVLQDHRMLQPTAEGKAIWRATVTSSTG
WSHSFTLLRLAPALIWEPDERPPPFAGTVAIDTAPADKEAEASLPLPVDGKTSTVESQEAPPWEGGSIAP
SPQAKAPAMPDVMEDMLAMVGMGDSPDATQNAEAILDKAPAAPPNTAAPVPAPVPVPTSSATQPSGEHFM
AWLQQGIASHRLIINDAKALVHTVSDTGYLVSPGVFQRYAQEHLQVGALAKQENLQDWQWVQKRFERLQL
HRKHANGLNIWTCEVTGPRKSRRLHGYLLQDPAGLFGELPPNNPYLSVQP

  Protein domains


Predicted by InterproScan.

(485-607)

(27-340)


  Protein structure



No available structure.




T4CP


ID   17129 GenBank   CAJ24054
Name   t4cp2_XCV2377_ICEXcaVe85-1 insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 81015.72 Da        Isoelectric Point: 7.3316

>CAJ24054.1 putative membrane protein [Xanthomonas euvesicatoria pv. vesicatoria str. 85-10]
MSGKQPVEVLLRPAVELYTVAACAGAAFLSLVAPWSLALSPAMGVGSALAFGAYGAIRYRDARVILRYRR
NIRRLPRYVMTSRDVPVSQQRLFVGRGFLWEQKHTHRLMQTYRPEFRRYAEPTPAYRLARRLEERLEFAP
FPLSRLPALTGWDVPFNPVRPLPPVGGLPRLHGIEPDEVDVSLPLGERVGHSLVLGTTRVGKTRLAELFV
TQDIRRKNAADEHEVVIVIDPKGDADLLKRMYVEAKRAGREREFYVFHLGWPDISARYNAVGRFGRISEV
ATRVAGQLSGEGNSAAFREFAWRFVNIIARALVELGQRPDYMLIQRHVINIDALFIEYAQHYFAKTEPKA
WEVIVQIEAKLNEKNIPRNMIGREKRVVALEQYLSQARNYDPVLDGLRSAVRYDKTYFDKIVASLLPLLE
KLTSGKIAQLLAPNYSDLTDPRPIFDWMQIIRKRAIVYVGLDALSDAEVAAAVGNSMFSDLVSVAGHIYK
HGIDDGLPGASAGARVPINVHADEFNELMGDEFIPLINKGGGAGLQVTAYTQTLSDIEARIGNRAKAGQV
IGNFNNLFMLRVREAATAELLTRQLPKVEVYTTTIVSGATDSSDIRGATDFTSNTQDRISMSSVPMIEPS
HVVGLPKGQCFALLQGGQLWKVRMPLPAPDPDEVMPADLQQLAGYMRQSYSEATQWWEFTSSPALQETGL
PDDLLDDAAPAEPPAVATVSDDGAGNEASQ

  Protein domains


Predicted by InterproScan.

(508-636)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 2689317..2709471

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XCV2355 2684884..2685381 + 498 CAJ24032 conserved hypothetical protein -
XCV2356 2685446..2687278 - 1833 CAJ24033 conserved hypothetical protein -
XCV2357 2687592..2688218 + 627 CAJ24034 conserved hypothetical protein -
XCV2358 2688231..2688848 + 618 CAJ24035 conserved hypothetical protein -
XCV2359 2688948..2689310 + 363 CAJ24036 putative membrane protein -
XCV2360 2689317..2690840 - 1524 CAJ24037 putative membrane protein tfc19
XCV2361 2690855..2691226 - 372 CAJ24038 putative membrane protein tfc18
XCV2362 2691223..2692620 - 1398 CAJ24039 putative membrane protein tfc22
XCV2363 2692630..2693577 - 948 CAJ24040 putative secreted protein tfc23
XCV2364 2693574..2694020 - 447 CAJ24041 putative secreted protein tfc24
XCV2365 2694183..2694677 - 495 CAJ24042 RadC family DNA repair protein -
XCV2366 2694859..2695644 - 786 CAJ24043 putative membrane protein -
XCV2367 2695658..2698525 - 2868 CAJ24044 conserved hypothetical protein virb4
XCV2368 2698525..2698965 - 441 CAJ24045 putative secreted protein tfc15
XCV2369 2698946..2700376 - 1431 CAJ24046 putative secreted protein tfc14
XCV2370 2700366..2701280 - 915 CAJ24047 putative secreted protein tfc13
XCV2371 2701277..2701969 - 693 CAJ24048 putative secreted protein tfc12
XCV2372 2701966..2702364 - 399 CAJ24049 putative membrane protein tfc11
XCV2373 2702377..2702733 - 357 CAJ24050 putative membrane protein tfc10
XCV2374 2702751..2702984 - 234 CAJ24051 putative membrane protein tfc9
XCV2375 2702981..2703361 - 381 CAJ24052 putative secreted protein tfc8
XCV2376 2703460..2704209 - 750 CAJ24053 putative membrane protein tfc7
XCV2377 2704206..2706398 - 2193 CAJ24054 putative membrane protein -
XCV2378 2706403..2706942 - 540 CAJ24055 putative secreted protein tfc5
XCV2379 2706939..2707529 - 591 CAJ24056 putative lytic transglycosylase precursor virB1
XCV2380 2707511..2708236 - 726 CAJ24057 putative secreted protein tfc3
XCV2381 2708249..2708818 - 570 CAJ24058 putative secreted protein -
XCV2382 2708872..2709471 - 600 CAJ24059 putative secreted protein tfc2
XCV2383 2709660..2710748 - 1089 CAJ24060 hypothetical protein -
XCV2384 2710765..2712960 - 2196 CAJ24061 putative cytochrome precursor -
XCV2385 2713288..2713974 - 687 CAJ24062 hypothetical protein -


Host bacterium


ID   421 Element type   ICE (Integrative and conjugative element)
Element name   ICEXcaVe85-1 GenBank   AM039952
Element size   5178466 bp Coordinate of oriT [Strand]   101397..101714 [+]
Host bacterium   Xanthomonas campestris pv. vesicatoria Coordinate of element   2631723..2740954

Cargo genes


Drug resistance gene   aph(6)-Id, aph(3'')-Ib
Virulence gene   -
Metal resistance gene   golS, golT
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -