Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   200474
Name   oriT_ICEBxeLB400-1 in_silico
Organism   Paraburkholderia xenovorans LB400
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   CP000270 (52372..52611 [-], 240 nt)
oriT length   240 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 240 nt

>oriT_ICEBxeLB400-1
TGTGGGTGTCCTAGTGGCGGGCCGACCGGGAGGCCTTTTGCCTTTTCGAGGTAGGGCCTTTCCCCTTGCAGCCCCTTCCATTGCCCTTTCGGCCCTTTGGCCTTTAACCATTTGGATATAGGGCGGCGTGCGCTTGCCGTCCACCACCAATGCGGGTTGCAGCGAGCCGGATTGGTGCGTGAAGGCTCGCTGTTCCCCCCCATACGATGGCCCGATTCTTGGACTCTGGAGAGCATCATG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   14511 GenBank   ABE31780
Name   TraI_2_Bxe_A1173_ICEBxeLB400-1 insolico UniProt ID   _
Length   615 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 615 a.a.        Molecular weight: 67419.64 Da        Isoelectric Point: 7.4238

>ABE31780.1 Conserved hypothetical protein [Paraburkholderia xenovorans LB400]
MLSLFQRKRPVVASAPSPAPSNDLPKGRLRPESAASLLATPRRQKLLEHIWQRTSLSRKQFATLYRAPLE
RYAELVQRFPASEAHHHAYPGGMLDHGLEIVAYALKLRQSHLLPAGSTPEDQAAQSEAWTAAVAYAALLH
DVGKLAVDLHVELADGSTWHPWHGPLRQPYRFRYREDREYRLHSAATGLLYRQLLDAQLLDWLSGYPALW
GPLLYVLAGQYEHAGVLGELVVQADRASVAQELGGDPARAMAAPKHALQRKLLDGLRYLLKEELKLNQPE
ASDGWLTEDALWLVSKTVSDKLRAHLLSQGIDGIPANNTAVFNVLQDHGMLHPTTDGKAVWRATVTSATG
WSHSFTLLRLAPALIWEPGERPVPFAGTVAIDAVPNDKSVAQPAAVAETTQEGQEAPPWESSSVAVPSPA
TQTVPDVLEDMLAMVGMGNSSGTQQDEEATSHAADATPSEAPMPAMAAASPPSPVPPAAPSSATAQPSGE
HFMAWLKQGIVSRRLIINDAKALVHTVSDTAYLVSPGVFQRYAREHPQVGMLAKQESQQDWQWVQKRFEK
LQLHRKHINGLNVWACSVTGSRKSRQLHGYLLRDPLPLFGKVPPNNPYLSILQSI

  Protein domains


Predicted by InterproScan.

(27-340)

(488-610)


  Protein structure



No available structure.




T4CP


ID   17127 GenBank   ABE31758
Name   t4cp2_Bxe_A1195_ICEBxeLB400-1 insolico UniProt ID   _
Length   728 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 728 a.a.        Molecular weight: 80862.63 Da        Isoelectric Point: 7.6092

>ABE31758.1 hypothetical protein Bxe_A1195 [Paraburkholderia xenovorans LB400]
MSGKQPVEVLLRPAVELYTVAACAGAAFLSLVAPWSLALSPAMGVGSALAFGAYGAIRYRDARVILRYRR
NIRRLPRYVMTSKDVPVSQQRLFVGRGFLWEQKHTHRLMQTYRPEFRRYVEPTPAYRLTRRLEERLEFAR
FPLSRLPKLTGWDVPFNPVRPLPPVGGLPRLHGIEPEEVDVSLPLGERVGHSLVLGTTRVGKTRLAELFV
TQDIRRKNAAGEHEVVIVIDPKGDADLLKRMYVEAQRAGREGEFYVFHLGWPDISARYNAVGRFGRISEV
ATRIAGQLSGEGNSAAFREFAWRFVNIIARALVELGQRPDYMLIQRHVINIDALFIEYAQHYFAKTEPKA
WEVIVQIEAKLNEKNIPRNMIGREKRVVALEQYLSQARNYDPVLDGLRSAVRYDKTYFDKIVASLLPLLE
KLTSGKIAQLLAPNYSDLADPRPIFDWMQVIRKRAIVYVGLDALSDAEVAAAVGNSMFSDLVSVAGHIYK
HGIDDGLPGASAGARVPINVHADEFNELMGDEFIPLINKGGGAGLQVTAYTQTLSDIEARIGNRAKAGQV
IGNFNNLFMLRVRETATAELLTRQLPKVEVYTTTIVSGATDSSDIRGATDFTSNTQDRISMSSVPMIEPS
HIVGLPKGQCFALLQGGQLWKVRMPLPAPDPDEVMPQDLQQLAGYMRQSYSEATQWWEFTSSPALQDGAL
PDDLLDDAAAVEPGLVATDDSAGNEASP

  Protein domains


Predicted by InterproScan.

(508-636)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 3589842..3610530

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Bxe_A1203 3585210..3586319 + 1110 ABE31750 Conserved hypothetical, plasmid-related protein -
Bxe_A1202 3586392..3588698 + 2307 ABE31751 Putative, plasmid-related, DNA/RNA helicase -
Bxe_A1201 3588812..3589681 + 870 ABE31752 hypothetical protein -
Bxe_A1200 3589842..3590441 + 600 ABE31753 Conserved hypothetical protein tfc2
Bxe_A1199 3590438..3591088 + 651 ABE31754 hypothetical protein -
Bxe_A1198 3591103..3591822 + 720 ABE31755 Conserved hypothetical protein tfc3
Bxe_A1197 3591804..3592394 + 591 ABE31756 Conserved hypothetical protein virB1
Bxe_A1196 3592391..3592939 + 549 ABE31757 Conserved hypothetical protein tfc5
Bxe_A1195 3592944..3595130 + 2187 ABE31758 hypothetical protein -
Bxe_A1194 3595127..3595876 + 750 ABE31759 Conserved hypothetical protein tfc7
Bxe_A1193 3595913..3596317 - 405 ABE31760 hypothetical protein -
Bxe_A1192 3596503..3596886 + 384 ABE31761 Plasmid conserved hypothetical protein, RAQPRD tfc8
Bxe_A1191 3596883..3597116 + 234 ABE31762 hypothetical protein tfc9
Bxe_A1190 3597127..3597492 + 366 ABE31763 Conserved hypothetical protein tfc10
Bxe_A1189 3597505..3597915 + 411 ABE31764 Conserved hypothetical protein tfc11
Bxe_A1188 3597912..3598604 + 693 ABE31765 Conserved hypothetical protein tfc12
Bxe_A1187 3598601..3599533 + 933 ABE31766 Conserved hypothetical protein tfc13
Bxe_A1186 3599523..3600941 + 1419 ABE31767 Conserved hypothetical protein tfc14
Bxe_A1185 3600922..3601362 + 441 ABE31768 Conserved hypothetical protein tfc15
Bxe_A1184 3601362..3604229 + 2868 ABE31769 Conserved hypothetical protein virb4
Bxe_A1183 3604243..3605007 + 765 ABE31770 Conserved hypothetical protein -
Bxe_A1182 3605191..3605685 + 495 ABE31771 DNA repair protein RadC -
Bxe_A1181 3605844..3606290 + 447 ABE31772 Conserved hypothetical protein tfc24
Bxe_A1180 3606287..3607234 + 948 ABE31773 Conserved hypothetical protein tfc23
Bxe_A1179 3607244..3608641 + 1398 ABE31774 Conserved hypothetical protein tfc22
Bxe_A1178 3608638..3608997 + 360 ABE31775 hypothetical protein tfc18
Bxe_A1177 3609013..3610530 + 1518 ABE31776 Conserved hypothetical protein tfc19
Bxe_A1176 3610544..3610921 - 378 ABE31777 hypothetical protein -
Bxe_A1175 3610949..3611407 - 459 ABE31778 Conserved hypothetical protein -
Bxe_A1174 3611407..3611793 - 387 ABE31779 transcriptional regulator, AbrB family -
Bxe_A1173 3612030..3613877 + 1848 ABE31780 Conserved hypothetical protein -
Bxe_A1171 3614392..3615198 - 807 ABE31781 transcriptional regulator, AraC family -


Host bacterium


ID   419 Element type   ICE (Integrative and conjugative element)
Element name   ICEBxeLB400-1 GenBank   CP000270
Element size   4895836 bp Coordinate of oriT [Strand]   52372..52611 [-]
Host bacterium   Paraburkholderia xenovorans LB400 Coordinate of element   3559420..3682252

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   bphA1
Symbiosis gene   -
Anti-CRISPR   -