Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200465
• Name: oriT_ICESpa15912-1
• Organism: Streptococcus parasanguinis ATCC 15912
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP002843 (11030..11176 [-], 147 nt)
• oriT length: 147 nt
• IRs (inverted repeats): 124..129, 133..138 (AAAGTG..CACTTT) 111..116, 124..129 (CACTTT..AAAGTG) 9..15, 28..34 (AACCCCC..GGGGGTT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 147 nt

>oriT_ICESpa15912-1
GAAACTTCAACCCCCGTTTTCTAATAGGGGGGTTACATTTGGCAAAAACGCCAAGTCCACCCCTCCTCATTCCTTATGGGAGTTGAGTTTTCAATTTTTATGAAGTGTGTCACTTTCGTCAAAAAAGTGTGTCACTTTGTTAGAAAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_HMPREF0833_11846_ICESpa15912-1

• ID: 14489
• GenBank: AEH56877
• Name: Rep_trans_HMPREF0833_11846_ICESpa15912-1
• UniProt ID: _
• Length: 410 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 410 a.a.
• Molecular weight: 48370.83 Da
• Isoelectric Point: 5.7312

>AEH56877.1 DNA-binding helix-turn-helix protein [Streptococcus parasanguinis ATCC 15912]
MDKISPFHIKNFRKQTGLSQKAFAQAVDLPTRTYRSYETGERGLTIDKFRELKERLGYYQDCDKNSLRAQ
IDYLRLTFPRLKDLDAFCENFLHCHLSEFTDQETRLMNYTHLWQRGNIWIFDFFDKSVTNDYQTCLQLSG
QGCRELELLLEDKGITWQIFLQNILYSYEDVRVKRLDIALDELYKGYGHEDEQIQIPKLIDKLYSKEIVL
DTIKKWNITGGGSFTDNEDMEANHGLSIYFGSRQSQLYFNFYEKRYEIARMENISLDESLEIFGIWNRYE
LRFSDQKAQGIVEEYINGVDLGEIARGIVNKEIQVYDGLTRFGAYKPDEKWQRLFGGVEPLKLSTNPQPY
SIERTIRWLTYQVANSLALVTEADKILQTEYMKMIQNSGEITDRGKAMLRLLKANKHYEH

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (172-384)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (69-162)

• Helix-turn-helix (HTH_3) (9-57)

Protein structure

No available structure.

T4CP

tcpA_HMPREF0833_11847_ICESpa15912-1

• ID: 17097
• GenBank: AEH56878
• Name: tcpA_HMPREF0833_11847_ICESpa15912-1
• UniProt ID: _
• Length: 562 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 562 a.a.
• Molecular weight: 65512.03 Da
• Isoelectric Point: 8.8737

>AEH56878.1 FtsK/SpoIIIE family protein [Streptococcus parasanguinis ATCC 15912]
MKLFTYRGKRVRVFHRKLKGITWFIWWFPFLLAISYYSYLHLEELKSHPLPQGYFLGAGLLFSLLLSWFI
NRVCYRKMLFFQKLNSLRILSRFLLENNLYLVKKVRRENKIIEKITLPQVYIKQSRYKIEVSFILEGNKF
QDRFLNLGATLEVMFNGDFRNKTFDNRFIKYEIAINRIDSRITIDEVKVKGSKLQLMKDVSWDYIEEPHL
LIGGGTGGGKTVVLMTIIYALAKIGFVDICDPKNSDLAGLKKIPVFHGRVYTSKEDIINCFKENVEFMEK
RYERMSTSPRFQAGKNFTHYDMKPKFILVDEWAALMAKIDRDYSQQSELMEYLSQLVLEGRQAGVFIIFA
MQRPDGEFIKTALRDNFMKRLSVGHLESTGYDMMFGDANKTKEFKKLDEINGVKVKGRGYIANNGELAGE
FFSPYVPLDQGFSFYDAYSKIPIMEFEGEEFSVFEEYKPPLETIDPIEEEETIENEPNKRPLKEFADEQN
LKMPTLRKIIYLLTEQGIIFERTVSAILVDPFQEKLLLEILAQFEEGGRRSYPKAVEATIVHHGLGQGQG
QA

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (200-287)

Protein structure

No available structure.

Host bacterium

• ID: 410
• Element type: ICE (Integrative and conjugative element)
• Element name: ICESpa15912-1
• GenBank: CP002843
• Element size: 2153652 bp
• Coordinate of oriT [Strand]: 11030..11176 [-]
• Host bacterium: Streptococcus parasanguinis ATCC 15912
• Coordinate of element: 1960240..1989074

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -