Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200463 |
| Name | oriT_ICEBsa18170-1  |
| Organism | Bacteroides salanitronis DSM 18170 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP002530 (27050..27246 [-], 197 nt) |
| oriT length | 197 nt |
| IRs (inverted repeats) | 98..105, 116..123 (GGCTTTCC..GGAAAGCC) 47..53, 58..64 (GCTCAAA..TTTGAGC) 10..17, 20..27 (TATGTTCA..TGAACATA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 197 nt
>oriT_ICEBsa18170-1
ACGGATTTTTATGTTCACCTGAACATAGCAAGATGTCTTTTCAGCCGCTCAAAATGATTTGAGCATCCCGGAAAAGCACTTGCCCTTGCAGGGGACGGGCTTTCCCTCCGAAGTCGGAAAGCCTTTCAGAACTGCGGTGCCGTGAACAGAAGCGGCGGCTTATGCCGTCAATCCGCTAAATCCAAAGTGATATGAAA
ACGGATTTTTATGTTCACCTGAACATAGCAAGATGTCTTTTCAGCCGCTCAAAATGATTTGAGCATCCCGGAAAAGCACTTGCCCTTGCAGGGGACGGGCTTTCCCTCCGAAGTCGGAAAGCCTTTCAGAACTGCGGTGCCGTGAACAGAAGCGGCGGCTTATGCCGTCAATCCGCTAAATCCAAAGTGATATGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14487 | GenBank | ADY37081 |
| Name | Relaxase_Bacsa_2544_ICEBsa18170-1 |
UniProt ID | _ |
| Length | 415 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 415 a.a. Molecular weight: 46816.27 Da Isoelectric Point: 9.8838
>ADY37081.1 Relaxase/mobilization nuclease family protein [Phocaeicola salanitronis DSM 18170]
MVAKISVGNSLYGAIAYNGEKINEAQGRLLTTNRIYNDGSGTVDISKAMEGFHTFLPPQMKVEKPVVHIS
LNPHPEDVLTDIELQNIAREYLEKLGFGNQPYLVFKHEDIDRHHLHIVTVNVDENGKRLNRDFLYRRSDR
IRRELEQKYGLHPAERKNQGLDNPLRKVDASAGDVKKQVGNTVKALNGQYRFQTMGEYRALLSLYNMTVE
EARGNVRGREYHGLVYSVTDDRGNKVGNPFKSSLFGKSAGYEAVQKKFVRSKSEIKDRKLADMTKRTVLS
VLQGTYDKDRFISRLKEKGIDTVLRYTEEGRIYGATFIDHRTGCVLNGSRMGKELSANALQEHFTLPYAG
QPPIPLSIPADVADKVYGQTAYDREDMSGGMGLLTPEGPATDAEEEAFIRAMKRKKKKKRKGLGM
MVAKISVGNSLYGAIAYNGEKINEAQGRLLTTNRIYNDGSGTVDISKAMEGFHTFLPPQMKVEKPVVHIS
LNPHPEDVLTDIELQNIAREYLEKLGFGNQPYLVFKHEDIDRHHLHIVTVNVDENGKRLNRDFLYRRSDR
IRRELEQKYGLHPAERKNQGLDNPLRKVDASAGDVKKQVGNTVKALNGQYRFQTMGEYRALLSLYNMTVE
EARGNVRGREYHGLVYSVTDDRGNKVGNPFKSSLFGKSAGYEAVQKKFVRSKSEIKDRKLADMTKRTVLS
VLQGTYDKDRFISRLKEKGIDTVLRYTEEGRIYGATFIDHRTGCVLNGSRMGKELSANALQEHFTLPYAG
QPPIPLSIPADVADKVYGQTAYDREDMSGGMGLLTPEGPATDAEEEAFIRAMKRKKKKKRKGLGM
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 17095 | GenBank | ADY37080 |
| Name | t4cp2_Bacsa_2543_ICEBsa18170-1 |
UniProt ID | _ |
| Length | 668 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 668 a.a. Molecular weight: 76618.51 Da Isoelectric Point: 6.9934
>ADY37080.1 TRAG family protein [Phocaeicola salanitronis DSM 18170]
MSQEDDLRALAKIMDFLRAVSIILVVMNVYWFCYEAIRMWGVNIGVVDKILLNFDRTAGLFHSILYTKLF
SVLLLALSCLGTKGVKGEKITWGRIWTAFAVGFVLFFLNWWLLPLPLPLEAVTGLYVLTIGTGYVCLLMG
GLWMSRLLKHNLMEDVFNNENESFMQETRLIESEYSVNLPTRFYYRKRWNNGWINVVNPFRASIVLGTPG
SGKSYAVVNNFIKQQIEKGFSMYVYDFKFSDLSTIAYNHLLNHPEGYKVKPKFYVINFDDPRRSHRCNPI
HPDFMEDITDAYESAYTIMLNLNKTWVQKQGDFFVESPIILFASIIWYLKIYQNGKYCTFPHAIEFLNRR
YEDIFPILTSYPELENYLSPFMDAWLGGAAEQLMGQIASAKIPLSRMISPQLYWVMSDSEFTLDINNPEE
PKILCVGNNPDRQNIYGAALGLYNSRIVKLINKKGMLKSSVIIDELPTIYFKGLDNLIATARSNKVAVCL
GFQDFSQLVRDYGDKEAKVVMNTVGNIFSGQVVGETAKTLSERFGKVLQKRQSISINRQDVSTSINTQMD
ALIPPSKISGLTQGMFVGSVSDNFNERIEQKIFHCEIVVDAEKVKREESAYKKIPVITDFTDEDGNDHMK
ETVQANYRRIKEEVKQIVQDELERIAGDDNLKHLLQQK
MSQEDDLRALAKIMDFLRAVSIILVVMNVYWFCYEAIRMWGVNIGVVDKILLNFDRTAGLFHSILYTKLF
SVLLLALSCLGTKGVKGEKITWGRIWTAFAVGFVLFFLNWWLLPLPLPLEAVTGLYVLTIGTGYVCLLMG
GLWMSRLLKHNLMEDVFNNENESFMQETRLIESEYSVNLPTRFYYRKRWNNGWINVVNPFRASIVLGTPG
SGKSYAVVNNFIKQQIEKGFSMYVYDFKFSDLSTIAYNHLLNHPEGYKVKPKFYVINFDDPRRSHRCNPI
HPDFMEDITDAYESAYTIMLNLNKTWVQKQGDFFVESPIILFASIIWYLKIYQNGKYCTFPHAIEFLNRR
YEDIFPILTSYPELENYLSPFMDAWLGGAAEQLMGQIASAKIPLSRMISPQLYWVMSDSEFTLDINNPEE
PKILCVGNNPDRQNIYGAALGLYNSRIVKLINKKGMLKSSVIIDELPTIYFKGLDNLIATARSNKVAVCL
GFQDFSQLVRDYGDKEAKVVMNTVGNIFSGQVVGETAKTLSERFGKVLQKRQSISINRQDVSTSINTQMD
ALIPPSKISGLTQGMFVGSVSDNFNERIEQKIFHCEIVVDAEKVKREESAYKKIPVITDFTDEDGNDHMK
ETVQANYRRIKEEVKQIVQDELERIAGDDNLKHLLQQK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2969622..2980177
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Bacsa_2544 | 2964751..2965998 | - | 1248 | ADY37081 | Relaxase/mobilization nuclease family protein | - |
| Bacsa_2545 | 2965977..2966405 | - | 429 | ADY37082 | hypothetical protein | - |
| Bacsa_2546 | 2967115..2967876 | + | 762 | ADY37083 | hypothetical protein | - |
| Bacsa_2547 | 2967879..2968319 | + | 441 | ADY37084 | conjugate transposon protein | - |
| Bacsa_2548 | 2968334..2968675 | + | 342 | ADY37085 | hypothetical protein | - |
| Bacsa_2549 | 2968692..2969420 | + | 729 | ADY37086 | hypothetical protein | - |
| Bacsa_2550 | 2969622..2969939 | + | 318 | ADY37087 | hypothetical protein | traE |
| Bacsa_2551 | 2969950..2970282 | + | 333 | ADY37088 | hypothetical protein | traF |
| Bacsa_2552 | 2970279..2972783 | + | 2505 | ADY37089 | bacteroides conjugation system ATPase, TraG family | virb4 |
| Bacsa_2553 | 2972824..2973204 | + | 381 | ADY37090 | hypothetical protein | traH |
| Bacsa_2554 | 2973228..2973857 | + | 630 | ADY37091 | hypothetical protein | traI |
| Bacsa_2555 | 2973861..2974865 | + | 1005 | ADY37092 | bacteroides conjugative transposon TraJ protein | traJ |
| Bacsa_2556 | 2974897..2975520 | + | 624 | ADY37093 | bacteroides conjugative transposon TraK protein | traK |
| Bacsa_2557 | 2975527..2975835 | + | 309 | ADY37094 | hypothetical protein | traL |
| Bacsa_2558 | 2975816..2977168 | + | 1353 | ADY37095 | bacteroides conjugative transposon TraM protein | traM |
| Bacsa_2559 | 2977203..2978189 | + | 987 | ADY37096 | bacteroides conjugative transposon TraN protein | traN |
| Bacsa_2560 | 2978192..2978767 | + | 576 | ADY37097 | Conjugative transposon protein TraO | traO |
| Bacsa_2561 | 2978775..2979677 | + | 903 | ADY37098 | hypothetical protein | traP |
| Bacsa_2562 | 2979674..2980177 | + | 504 | ADY37099 | hypothetical protein | traQ |
| Bacsa_2563 | 2980174..2980701 | + | 528 | ADY37100 | lysozyme | - |
| Bacsa_2564 | 2980742..2980993 | + | 252 | ADY37101 | hypothetical protein | - |
| Bacsa_2565 | 2981098..2982384 | - | 1287 | ADY37102 | transposase IS4 family protein | - |
| Bacsa_2566 | 2982575..2983375 | + | 801 | ADY37103 | rRNA (adenine-N(6)-)-methyltransferase | - |
Host bacterium
| ID | 310 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEBsaDSM18170-1 | GenBank | CP002530 |
| Element size | 4242803 bp | Coordinate of oriT [Strand] | 27050..27246 [-] |
| Host bacterium | Bacteroides salanitronis DSM 18170 | Coordinate of element | 2939351..2990000 |
Cargo genes
| Drug resistance gene | tet(Q), erm(F) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA9 |