Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200460
• Name: oriT1_ICESgal1
• Organism: Streptococcus gallolyticus UCN34
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): FN597254 (45728..45860 [-], 133 nt)
• oriT length: 133 nt
• IRs (inverted repeats): 65..70, 83..88 (AAATCC..GGATTT) 6..12, 23..29 (ACCCCCC..GGGGGGT)
• Location of nic site: 75..76
• Conserved sequence flanking the nic site: TTTGGTTACA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 133 nt

>oriT1_ICESgal1
ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_GALLO_1670_ICESgal1

• ID: 14480
• GenBank: CBI14161
• Name: Rep_trans_GALLO_1670_ICESgal1
• UniProt ID: _
• Length: 416 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 416 a.a.
• Molecular weight: 49021.47 Da
• Isoelectric Point: 5.2896

>CBI14161.1 putative transcriptional regulator, Cro/CI family [Streptococcus gallolyticus UCN34]
MEAYYLQRFRKKTGLNQSDFAKTVGISQSLISRYELGKKTLSVETFHKIKSAFGYFDCDKDRLRYMIDYL
RITFKSVRDLEKFTREYLLIPFREFGSYETKLMMYTHLWKRGDIWIFDYHDKFETNNYQITIQLSGSGCR
QMEVMLEHYGLTWRDLLANMRSAFGDSMKVTRLDIAIDELYLGVGRENEQFQLEDLISKYYKKELYFDKL
RKWNYIGGGSLGSSNNDYEEERQGISIYFGSRQSEMYFNFYEKRYELAKQERITVNEGLEVFNIWNRYEI
RLAQKKADSIVNEYINGVDLAILGRGLINSRITVYDGTNEYGAYLVDSKWQCLFGGSEGVVISIDPEPYD
IRRTIAWLRYQVSNSLALVREFDKIVGEDNLDYVLNSGELTDEAKRTLVAVEAFVRLNQGEYDDYA

Protein domains

Predicted by InterproScan.

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (66-158)

• Replication initiation factor (Rep_trans) (168-382)

• Helix-turn-helix (HTH_3) (6-54)

Protein structure

No available structure.

Mob_Pre_GALLO_1685_ICESgal1

• ID: 14481
• GenBank: CBI14176
• Name: Mob_Pre_GALLO_1685_ICESgal1
• UniProt ID: _
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49660.33 Da
• Isoelectric Point: 9.9693

>CBI14176.1 putative plasmid recombination/mobilization protein [Streptococcus gallolyticus UCN34]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

No available structure.

Rep_trans_GALLO_1696_ICESgal1

• ID: 14482
• GenBank: CBI14187
• Name: Rep_trans_GALLO_1696_ICESgal1
• UniProt ID: _
• Length: 401 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 401 a.a.
• Molecular weight: 47398.10 Da
• Isoelectric Point: 6.5091

>CBI14187.1 putative transcriptional regulator, Cro/CI family (Tn916) [Streptococcus gallolyticus UCN34]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

Protein domains

Predicted by InterproScan.

• Helix-turn-helix (HTH_3) (15-53)

• Replication initiation factor (Rep_trans) (169-373)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (73-161)

Protein structure

No available structure.

Auxiliary protein

CBI14167_ICESgal1

• ID: 7908
• GenBank: CBI14167
• Name: CBI14167_ICESgal1
• UniProt ID: _
• Length: 405 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 405 a.a.
• Molecular weight: 47021.92 Da
• Isoelectric Point: 9.7978

>CBI14167.1 putative integrase [Streptococcus gallolyticus UCN34]
MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKLVATDRVPAGKRDCISLREKIAELQKD
IHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSE
NGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYS
KNYDEILILLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQ
AFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFC
TNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA

Protein domains

Predicted by InterproScan.

• Phage integrase family (Phage_integrase) (190-383)

• DNA binding domain of tn916 integrase (Integrase_DNA) (1-71)

Protein structure

No available structure.

T4CP

tcpA_GALLO_1672_ICESgal1

• ID: 17088
• GenBank: CBI14163
• Name: tcpA_GALLO_1672_ICESgal1
• UniProt ID: _
• Length: 569 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 569 a.a.
• Molecular weight: 65680.56 Da
• Isoelectric Point: 7.3661

>CBI14163.1 putative DNA segregation ATPase, FtsK/SpoIIIE family [Streptococcus gallolyticus UCN34]
MRLLPEYRGRRIRPYMRKLNVTLAFVFSFVLLLPSIAFGYVNIDALKAVDVKTIVILAVLSVLAIVLGIW
FSWFFREQTPFGKKVDRLGILSKYFFEHGFYYEKKRSGQNVKSKIRYPKIYVKQRKYDLDISFEMAGNKF
QDKFSKMGGELEKTLFMDFMETVDDVKYKTYTMAYQAFLNRINVTDVTYEKGKGLLLMKNFYWDFDSDPH
LLVAGGTGGGKTVLLQTLVLALAKIGVVDICDPKQADFVAIADMPAFKGRVAFDVEDIIQRFEKAEVIMM
ARYKFMNDERVRLKHKSLKKYYEYGLEPYFISCDELNALMAMLDYKQRERLDKSLGNILFLGRQAGVFDI
SAMQKPSREDLGSKLQANINMRLNVGRLDDGGYDIMYGEVNRNKDFKYLKYISGRRVYGRGYGAVMGDVA
REFFSPNMPKGFEFYDEFIKLERHENRFDPDENPEISVDIVNDKELLAVATEATNQANQISANKVQTEET
KYSLKEVADSIGASRPQVFKVVKMIEDDGYTTFEHDDTDKQVLTSSEVALVKELFDFKATNDLTWSNAVE
QYFTKESED

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (200-315)

Protein structure

No available structure.

tcpA_GALLO_1697_ICESgal1

• ID: 17089
• GenBank: CBI14188
• Name: tcpA_GALLO_1697_ICESgal1
• UniProt ID: _
• Length: 461 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 461 a.a.
• Molecular weight: 53370.27 Da
• Isoelectric Point: 9.0687

>CBI14188.1 Tn916 conserved hypothetical protein, putative translocase [Streptococcus gallolyticus UCN34]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (217-301)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1740511..1761877

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GALLO_1646	1738037..1739167	-	1131	CBI14137	putative integrase	-
GALLO_1647	1739222..1739452	-	231	CBI14138	putative excisionase	-
GALLO_1648	1739766..1740476	+	711	CBI14139	conserved hypothetical protein	-
GALLO_1649	1740511..1741413	-	903	CBI14140	Putative peptidoglycan linked serine rich protein (LPXTG motif)	prgC
GALLO_1650	1741431..1741997	-	567	CBI14141	conserved hypothetical secreted protein	-
GALLO_1651	1742008..1742706	-	699	CBI14142	putative sortase, A-type family	-
GALLO_1652	1742722..1744116	-	1395	CBI14143	conserved hypothetical secreted protein	-
GALLO_1653	1744118..1746262	-	2145	CBI14144	conserved hypothetical membrane protein	-
GALLO_1654	1746272..1748773	-	2502	CBI14145	putative conjugative transposon protein	virb4
GALLO_1655	1748915..1749445	+	531	CBI14146	hypothetical protein	-
GALLO_1656	1749431..1749697	-	267	CBI14147	hypothetical protein	-
GALLO_1657	1749757..1750167	-	411	CBI14148	conserved hypothetical protein	orf17b
GALLO_1658	1750171..1750437	-	267	CBI14149	conserved hypothetical protein	-
GALLO_1659	1750473..1751447	-	975	CBI14150	conserved hypothetical secreted protein	orf13
GALLO_1660	1751466..1751900	-	435	CBI14151	conserved hypothetical protein	-
GALLO_1661	1751917..1752285	-	369	CBI14152	hypothetical protein	-
GALLO_1662	1752285..1752500	-	216	CBI14153	hypothetical protein	-
GALLO_1663	1752511..1752993	-	483	CBI14154	conserved hypothetical protein	-
GALLO_1664	1753012..1753512	-	501	CBI14155	hypothetical protein	-
GALLO_1665	1753563..1753835	-	273	CBI14156	conserved hypothetical protein	-
GALLO_1666	1754053..1754463	-	411	CBI14157	putative transcriptional regulator	-
GALLO_1667	1754526..1754720	-	195	CBI14158	hypothetical protein	-
GALLO_1668	1754804..1755202	-	399	CBI14159	conserved hypothetical protein	-
GALLO_1669	1755217..1755480	-	264	CBI14160	hypothetical protein	-
GALLO_1670	1755507..1756757	-	1251	CBI14161	putative transcriptional regulator, Cro/CI family	-
GALLO_1671	1756783..1756992	-	210	CBI14162	Hypothetical protein	-
GALLO_1672	1757031..1758740	-	1710	CBI14163	putative DNA segregation ATPase, FtsK/SpoIIIE family	virb4
GALLO_1673	1758751..1759218	-	468	CBI14164	conserved hypothetical protein	-
GALLO_1674	1759218..1759529	-	312	CBI14165	conserved hypothetical protein	-
GALLO_1675	1759598..1761877	-	2280	CBI14166	Putative peptidoglycan linked protein (LPXTG motif)	prgB
GALLO_1676	1762086..1763303	-	1218	CBI14167	putative integrase	-
GALLO_1677	1763385..1763588	-	204	CBI14168	Tn916 conserved hypothetical protein, putative excisionase	-
GALLO_1678	1763572..1763823	+	252	CBI14169	Tn916 hypothetical protein	-
GALLO_1679	1764049..1764279	-	231	CBI14170	Tn916 conserved hypothetical protein	-
GALLO_1680	1764276..1764698	-	423	CBI14171	Tn916 conserved hypothetical protein	-
GALLO_1681	1765203..1765556	+	354	CBI14172	putative transcriptional regulator (Tn916)	-
GALLO_1682	1765616..1765804	-	189	CBI14173	Tn916 conserved hypothetical protein	-

Region 2: 1774651..1786061

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GALLO_1686	1770785..1772161	-	1377	CBI14177	tetracycline resistance protein	-
GALLO_1687	1772355..1774274	-	1920	CBI14178	tetracycline resistence protein TetM	-
GALLO_1688	1774290..1774376	-	87	CBI14179	TetM leader peptide	-
GALLO_1689	1774651..1775583	-	933	CBI14180	Tn916 conserved hypothetical protein	orf13
GALLO_1690	1775580..1776581	-	1002	CBI14181	Tn916 conserved hypothetical protein	orf14
GALLO_1691	1776578..1778755	-	2178	CBI14182	Tn916 conserved hypothetical protein	orf15
GALLO_1692	1778758..1781205	-	2448	CBI14183	Tn916 conserved hypothetical protein	virb4
GALLO_1693	1781189..1781581	-	393	CBI14184	Tn916 conserved hypothetical protein	orf17a
GALLO_1694	1781670..1782167	-	498	CBI14185	Tn916 conserved hypothetical protein	-
GALLO_1695	1782284..1782505	-	222	CBI14186	Tn916 conserved hypothetical protein	orf19
GALLO_1696	1782548..1783753	-	1206	CBI14187	putative transcriptional regulator, Cro/CI family (Tn916)	-
GALLO_1697	1783931..1785316	-	1386	CBI14188	Tn916 conserved hypothetical protein, putative translocase	virb4
GALLO_1698	1785345..1785728	-	384	CBI14189	Tn916 conserved hypothetical protein	orf23
GALLO_1699	1785747..1786061	-	315	CBI14190	Tn916 conserved hypothetical protein	orf23
GALLO_1700	1786084..1786203	-	120	CBI14191	Tn916 conserved hypothetical protein	-
GALLO_1701	1786497..1786814	-	318	CBI14192	conserved hypothetical protein	-
GALLO_1702	1786831..1787268	-	438	CBI14193	conserved hypothetical protein	-
GALLO_1703	1787265..1787519	-	255	CBI14194	conserved hypothetical protein	-
GALLO_1704	1787572..1787832	-	261	CBI14195	conserved hypothetical protein	-
GALLO_1705	1788021..1788431	-	411	CBI14196	conserved hypothetical protein	-
GALLO_1706	1788367..1788717	-	351	CBI14197	conserved hypothetical protein	-
GALLO_1707	1789020..1789607	-	588	CBI14198	conserved hypothetical protein	-
GALLO_1708	1790579..1790932	+	354	CBI14199	putative transcriptional regulator, Cro/CI family	-

Host bacterium

• ID: 407
• Element type: ICE (Integrative and conjugative element)
• Element name: ICESgal1
• GenBank: FN597254
• Element size: 2350911 bp
• Coordinate of oriT [Strand]: 45728..45860 [-]; 32255..32292 [-]
• Host bacterium: Streptococcus gallolyticus UCN34
• Coordinate of element: 1738037..1787519

Cargo genes

• Drug resistance gene: tet(L), tet(M)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -