Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200452
Name   oriT_ICESp23FST81 in_silico
Organism   Streptococcus pneumoniae ATCC 700669
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   FM211187 (61201..61333 [+], 133 nt)
oriT length   133 nt
IRs (inverted repeats)      65..70, 83..88  (AAATCC..GGATTT)
 6..12, 23..29  (ACCCCCC..GGGGGGT)
Location of nic site      75..76
Conserved sequence flanking the
  nic site    		  TTTGGTTACA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 133 nt

>oriT_ICESp23FST81
ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14454 GenBank   CAR69046
Name   Relaxase_SPN23F12430_ICESp23FST81 insolico UniProt ID   _
Length   609 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 609 a.a.        Molecular weight: 72987.25 Da        Isoelectric Point: 9.0973

>CAR69046.1 relaxase [Streptococcus pneumoniae ATCC 700669]
MVITKHFAIHGKNYRSKLVKYILNPSKTKNLALVSDFGMRNYLDFPSYKELVKMYNDNFLSNDGLYEFRH
DRQEVNQRRIYSHHIIQSFSPDDHLTPEQINRIGYETVKELTGGRFRFIVATHVDKGHIHNHIILNSIDQ
NSDKKFLWNYKSERNLRMVSDRLSKIAGAKIIENRYSHRQYEVYRKTNYKYEIKQRVYFLIENSKNFEDF
RKKAKALHLIIDFRHKHVTFFMTDSNMKQVVRDDKLNRKQPYNETYFKQKFVQREIINILEFLLPKMKNM
NELIQQAEFFDLKIIPKEKHVLFEFNGIKLSEQELGKMNQYSVSYFQDYFNNKNETFVLDNNNLIELYNK
EKLIKEKELPTEEVVWKSYQDFKRNRDAVHELEVELNLNQIEAVVDDGIYIKVQFGIRQEGLIFVPNIQI
NMEEEKVKVFLRETSSYYVYHKDSADKNRFMKGKTLIRQFTLQHEPQHMYRRIPLSKIKEKIEQLDFLIS
AENSPNDFEDITNDFIAQISYLENMIEQVQNKIDDLTNLEEVLLNNTTNSSSNLENSIQGKSSVDTIEKD
LYIYKGKIETLKEQHGEAINLFEMFNKTIKKYKKKQNMKSIEENEIHLE

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(12-264)


  Protein structure



No available structure.



ID   14455 GenBank   CAR69099
Name   orf20_SPN23F12970_ICESp23FST81 insolico UniProt ID   _
Length   401 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 401 a.a.        Molecular weight: 47398.10 Da        Isoelectric Point: 6.5091

>CAR69099.1 putative conjugative transposon replication initiation factor [Streptococcus pneumoniae ATCC 700669]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

  Protein domains


Predicted by InterproScan.

Helix-turn-helix (HTH_3)

(15-53)

Replication initiation factor (Rep_trans)

(169-373)

Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N)

(73-161)


  Protein structure



No available structure.




T4CP

ID   17062 GenBank   CAR69091
Name   traG_SPN23F12890_ICESp23FST81 insolico UniProt ID   _
Length   626 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 626 a.a.        Molecular weight: 72429.76 Da        Isoelectric Point: 9.4563

>CAR69091.1 putative conjugal transfer protein TraG [Streptococcus pneumoniae ATCC 700669]
MMYSGKKFLLFSLLGILLGYLFHRLTLLYDSYTGNSLDKWTHLLMEGQDEVLQSPWNVSFTGKSSAFFLL
GFVMMLLVYLYLETGKKQYREGIEYGSAHFGTLKEKKLFYGKEFSHDTILAQDVRLTLLDKKPPQYDRNK
NIAVIGGSGSGKTFRFVKPNLIQMNSSNIVVDPKDHLAEKTGKLFLEHGYQVKVLDLVNMKNSDGFNPFR
YIETENDLNRMLTVYFNNTKGSGSRSDPFWDEASMTLVRALASYLVDFYNPPKTREQLIEESRLSQKEHQ
NLLKRQKKEVEERKKRGRYPSFAEISKLIKHLSKGENQEKSVLEILFENYAKKYGTENFTMRNWADFQNY
KDKTLDSVIAVTTAKFALFNIQSVMDLTKRDTLDMKTWGKEKSMVYLVIPDNDSTFRFLSALFFSTVFQT
LTRQADIDFKGQLPLHVRVYLDEFANIGEIPDFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTI
LGNCDSLVYLGGNDEDTFKFMSGLLGKQTIDVRNTSRSFGQTGSGSLSHQKIARDLMTPDEVGNMKRHEC
LVRIANMPVFKSKKYNSTKHPNWKYLANQETDERWWDYQINPLNQSQENHLEGLRIRDLTFESSLK

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(131-575)

  Protein structure



No available structure.



ID   17063 GenBank   CAR69098
Name   orf21_SPN23F12960_ICESp23FST81 insolico UniProt ID   _
Length   461 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 461 a.a.        Molecular weight: 53370.27 Da        Isoelectric Point: 9.0687

>CAR69098.1 conjugative transposon FtsK/SpoIIIE-family protein [Streptococcus pneumoniae ATCC 700669]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD

  Protein domains


Predicted by InterproScan.

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(217-301)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1255346..1286209

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SPN23F12820 1253980..1254390 - 411 Protein_1216 putative uncharacterized protein (pseudogene) -
SPN23F12830 1254542..1255108 - 567 CAR69085 putative uncharacterized protein -
SPN23F12840 1255346..1258159 - 2814 CAR69086 putative conjugal transfer protein prgK
SPN23F12850 1258171..1260486 - 2316 CAR69087 putative conjugal transfer protein virb4
SPN23F12860 1260476..1260838 - 363 CAR69088 putative uncharacterized protein prgIc
SPN23F12870 1260892..1261746 - 855 CAR69089 putative uncharacterized protein prgHb
SPN23F12880 1261763..1262005 - 243 CAR69090 putative uncharacterized protein prgF
SPN23F12890 1262026..1263906 - 1881 CAR69091 putative conjugal transfer protein TraG virb4
SPN23F12900 1263903..1264391 - 489 CAR69092 putative uncharacterized protein gbs1365
SPN23F12910 1264381..1264680 - 300 CAR69093 putative uncharacterized protein -
SPN23F12920 1264963..1265799 - 837 CAR69094 conserved hypothetical protein -
SPN23F12930 1265799..1266389 - 591 CAR69095 conserved hypothetical protein -
SPN23F12940 1266820..1267134 + 315 CAR69096 conjugative transposon protein orf23
SPN23F12950 1267153..1267536 + 384 CAR69097 conjugative transposon protein orf23
SPN23F12960 1267565..1268950 + 1386 CAR69098 conjugative transposon FtsK/SpoIIIE-family protein virb4
SPN23F12970 1269128..1270333 + 1206 CAR69099 putative conjugative transposon replication initiation factor -
SPN23F12980 1270376..1270597 + 222 CAR69100 conjugative transposon protein orf19
SPN23F12990 1270714..1271211 + 498 CAR69101 conjugative transposon protein -
SPN23F13000 1271462..1271692 + 231 CAR69102 putative conjugative transposon membrane protein orf17a
SPN23F13010 1271676..1274123 + 2448 CAR69103 conjugative transposon ATP/GTP-binding protein virb4
SPN23F13020 1274126..1276276 + 2151 CAR69104 conjugative transposon membrane protein orf15
SPN23F13030 1276295..1277296 + 1002 CAR69105 putative cell wall hydrolase orf14
SPN23F13040 1277311..1278225 + 915 CAR69106 putative conjugative transposon exported protein orf13
SPN23F13050 1278602..1280521 + 1920 CAR69107 conjugative transposon tetracycline resistance protein -
SPN23F13060 1280867..1281220 - 354 CAR69108 putative conjugative transposon regulatory protein -
SPN23F13061 1281350..1281496 + 147 CAR69109 putative uncharacterized protein -
SPN23F13070 1281725..1282147 + 423 CAR69110 putative conjugative transposon regulatory protein -
SPN23F13080 1282835..1283038 + 204 CAR69111 excisionase -
SPN23F13090 1283120..1284336 + 1217 Protein_1244 putative integrase (pseudogene) -
SPN23F13110 1284551..1285138 - 588 CAR69113 putative CAAX amino terminal protease -
SPN23F13120 1285141..1285374 - 234 CAR69114 putative uncharacterized protein -
SPN23F13130 1285387..1285767 - 381 CAR69115 putative uncharacterized protein -
SPN23F13140 1285760..1286209 - 450 CAR69116 putative uncharacterized protein gbs1369
SPN23F13150 1286193..1287551 - 1359 CAR69117 putative DNA methylase -
SPN23F13160 1287650..1288429 - 780 CAR69118 putative replication initiator protein -
SPN23F13170 1288426..1288599 - 174 CAR69119 putative uncharacterized protein -
SPN23F13190 1288801..1289169 - 369 CAR69120 50S ribosomal protein L7/L12 -
SPN23F13200 1289245..1289745 - 501 CAR69121 50S ribosomal protein L10 -

Host bacterium

ID   401 Element type   ICE (Integrative and conjugative element)
Element name   ICESp23FST81 GenBank   FM211187
Element size   2221315 bp Coordinate of oriT [Strand]   61201..61333 [+]
Host bacterium   Streptococcus pneumoniae ATCC 700669 Coordinate of element   1207785..1288800

Cargo genes

Drug resistance gene   cat(pC194), tet(M)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21