Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   200448
Name   oriT_ICEAxyA8-1 in_silico
Organism   Achromobacter xylosoxidans A8
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   CP002287 (57136..57603 [-], 468 nt)
oriT length   468 nt
IRs (inverted repeats)      137..145, 152..160  (GATGCGGCC..GGCCGCATC)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 468 nt

>oriT_ICEAxyA8-1
AGCCGGGAAAGCCTGGACCAGCTCGGCGTAGCGTTCCAGTGGCGCCCGGTACAAGGTGGCGAACTGCTTACGTGAGAGCGACGTGCGCTGCCAGATGTGCTCCAGCAGCTTCTGCCGGCGCGGGGTGGCCAGCAGCGATGCGGCCGACTCGGGCCGCATCAACCCTTTCGGGAGGTCGGTGACCGATGGTGGCGTCGGAGCAGCAGCAACCGCGGGCCGTTTTCGCTGGAACAGAGAGAGCATGCGGGTGTCCTGTCGCGGGCCGAGCGGGGAGGCCTTTTTGCCTTTTCGGGATAGAGCCTTTCCCCTTGCACCCCATTCCCTTGCCATTTCGACCCTTTACAGCCTTTGGGTATAGGGCGACGGGTGCTGACCGTCCACCGCCAATGCGGGTTGCAGCAGGGCCGGATTGATGCAGGAAGGCTGGCTGTTCACGCCCTACGATGGGCCGATTCTTGGACTCGGAGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   14448 GenBank   ADP14461
Name   TraI_2_AXYL_01117_ICEAxyA8-1 insolico UniProt ID   _
Length   625 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 625 a.a.        Molecular weight: 68562.74 Da        Isoelectric Point: 7.2249

>ADP14461.1 hypothetical protein AXYL_01117 [Achromobacter xylosoxidans A8]
MLSLFQRKRPAVAAAPTPPSVTDLPKGLMRPESAASLLATPRRQKLLEHIWQRTSLSRKQFATLYRAPLE
RYAELVQAFPASEAHHHAYPGGMLDHGLEIVAYSLKLRQSHLLPIGASPEDQAAQAEAWTAAVAYAALLH
DIGKIAVDLHVELADGSLWHPWYGPLHQPYRFRYRDDREYHLHSAATGLLYRQLLDTQLLDWLSGYPDLW
GPLLYVLAGQYEHAGVLGELVVQADRASVAQELGGDPARAMAAPKHALQRKLLDGLRYLLKEQLKLNQPE
ASDGWLTEDALWLVSKTVSDKLRAHLLSQGVDGIPANNTAVFNVLQDHGMLQPTPDGKAIWRATVTSSTG
WCHSFTLLRLGPALIWEPSERPALFAGTVAIDAAATDKGPDVSATTPPAGAQSASEGQEVPPWEGGNPSR
PAKGEPLPDAMEDMLSMVGMVNSPATRQDAEAPSEPVSAARTEATTLTAPQRSSPAPKPTTAPSGEHFMA
WLKQGIASRRLIINDAKALVHTASGTAYLVSPGVFQRYAQERPQVSALAKQESQQDWQWIQKRFERLQLH
RKQPNGLNIWTCEVTGPRKSRRLHGYLLEDPSLLFSETPPSNPYLSLLGETRHCEQTSGKQGAST

  Protein domains


Predicted by InterproScan.

(27-340)

(484-606)


  Protein structure



No available structure.




T4CP


ID   17052 GenBank   ADP14440
Name   t4cp2_AXYL_01096_ICEAxyA8-1 insolico UniProt ID   _
Length   729 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 729 a.a.        Molecular weight: 80937.58 Da        Isoelectric Point: 6.9414

>ADP14440.1 hypothetical protein AXYL_01096 [Achromobacter xylosoxidans A8]
MSGKQPVEVLLRPAVELYTVAACAGAAFLSLVAPWSLALSPAMGVGAALAFGAYGAIRYRDARVILRYRR
NIRRLPRYVMTSRDVPVSQQRLFVGRGFLWEQKHTHRLMQTYRPEFRRYVEPTPAYRLARRLEERLEFAP
FPLSRLVRLTGWDVSFNPVRPLPPVGGLPRLHGIEPDEVDVSLPLGERVGHSLVLGTTRVGKTRLAELFV
TQDIRRTNADGEHEVVIVIDPKGDADLLKRMYVEAKRAGREGEFYVFHLGWPEFSARYNAVGRFGRISEV
ATRIAGQLSGEGNSAAFREFAWRFVNIIARALVELGQRPDYMLIQRHVINIDALFMEYAQHYFAKTEPKT
WEVIVQIEAKLNEKNIPRNMIGREKRVVALEQYLSQARNYDPVLDGLRSAVRYDKTYFDKIVASLLPLLE
KLTSGKIAQLLAPNYSDLSDARPIFDWMQVIRKRAVVYVGLDALSDAEVAAAVGNSMFSDLVSVAGHIYK
HGIDDGLPGASAGTRIPINVHADEFNELMGDEFIPLINKGGGAGLQVTAYTQTLSDIEARIGNRAKAGQV
IGNFNNLFMLRVRETATAELLTRQLPKVEVYTTTIVSGATDASDIRGTTDFTSNTQDRISMSSVPMIEPS
HVVGLPKGQCFALLQGGQLWKVRMPLPAPDPDEVMPADLQQLAGYMRQSYSEATQWWEFTSSPALQEAAL
PDDLLDDAAPAEPDAVATGADDSTGEAAP

  Protein domains


Predicted by InterproScan.

(508-636)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 1163505..1183635

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXYL_01086 1160278..1161000 - 723 ADP14430 arsenical resistance protein ArsH 1 -
AXYL_01087 1160993..1161415 - 423 ADP14431 arsenate reductase (glutaredoxin) 1 -
AXYL_01088 1161432..1162508 - 1077 ADP14432 sodium bile acid symporter family protein 1 -
AXYL_01089 1162519..1163013 - 495 ADP14433 low molecular weight phosphotyrosine protein phosphatase family protein -
AXYL_01090 1163016..1163354 - 339 ADP14434 bacterial regulatory protein, ArsR family protein 1 -
AXYL_01091 1163505..1164071 + 567 ADP14435 PilL protein tfc2
AXYL_01092 1164068..1164712 + 645 ADP14436 hypothetical protein -
AXYL_01093 1164727..1165452 + 726 ADP14437 hypothetical protein tfc3
AXYL_01094 1165434..1166024 + 591 ADP14438 transglycosylase SLT domain protein 2 virB1
AXYL_01095 1166021..1166560 + 540 ADP14439 hypothetical protein tfc5
AXYL_01096 1166565..1168754 + 2190 ADP14440 hypothetical protein -
AXYL_01097 1168751..1169500 + 750 ADP14441 putative membrane protein 10 tfc7
AXYL_01098 1169599..1169982 + 384 ADP14442 hypothetical protein tfc8
AXYL_01099 1169979..1170212 + 234 ADP14443 hypothetical protein tfc9
AXYL_01100 1170229..1170588 + 360 ADP14444 putative membrane protein 11 tfc10
AXYL_01101 1170600..1170998 + 399 ADP14445 hypothetical protein tfc11
AXYL_01102 1170995..1171687 + 693 ADP14446 hypothetical protein tfc12
AXYL_01103 1171684..1172595 + 912 ADP14447 hypothetical protein tfc13
AXYL_01104 1172585..1174003 + 1419 ADP14448 hypothetical protein tfc14
AXYL_01105 1173984..1174433 + 450 ADP14449 signal recognition particle GTPase tfc15
AXYL_01106 1174433..1177327 + 2895 ADP14450 type IV secretory pathway, VirB4 component virb4
AXYL_01107 1177341..1178105 + 765 ADP14451 hypothetical protein -
AXYL_01108 1178281..1178775 + 495 ADP14452 DNA repair protein RadC 1 -
AXYL_01109 1178993..1179385 + 393 ADP14453 hypothetical protein tfc24
AXYL_01110 1179382..1180332 + 951 ADP14454 hypothetical protein tfc23
AXYL_01111 1180342..1181736 + 1395 ADP14455 hypothetical protein tfc22
AXYL_01112 1181733..1182104 + 372 ADP14456 putative membrane protein 12 tfc18
AXYL_01113 1182118..1183635 + 1518 ADP14457 TraG-like family protein tfc19
AXYL_01114 1183651..1184028 - 378 ADP14458 hypothetical protein -
AXYL_01115 1184055..1184513 - 459 ADP14459 hypothetical protein -
AXYL_01116 1184513..1184830 - 318 ADP14460 transcriptional regulator, AbrB family domain protein -
AXYL_01117 1185130..1187007 + 1878 ADP14461 hypothetical protein -
AXYL_01118 1187091..1187228 + 138 ADP14462 hypothetical protein -
AXYL_01119 1187262..1187519 + 258 ADP14463 copper-translocating P-type ATPase 1 -


Host bacterium


ID   399 Element type   ICE (Integrative and conjugative element)
Element name   ICEAxyA8-1 GenBank   CP002287
Element size   7013095 bp Coordinate of oriT [Strand]   57136..57603 [-]
Host bacterium   Achromobacter xylosoxidans A8 Coordinate of element   1127770..1210913

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   merR2, merT, merA, arsH, arsC, copS, copR, copA, cusA/ybdE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -