Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200446 |
| Name | oriT_ICEMlSymTONO  |
| Organism | Mesorhizobium loti |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | AP017605 (348729..348789 [-], 61 nt) |
| oriT length | 61 nt |
| IRs (inverted repeats) | 27..33, 37..43 (CCGCCTC..GAGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 61 nt
>oriT_ICEMlSymTONO
AAAAGGATGTGCAGACAAAGGCTTCGCCGCCTCATGGAGGCGGTGCCTTTATCTTGCCTTA
AAAAGGATGTGCAGACAAAGGCTTCGCCGCCTCATGGAGGCGGTGCCTTTATCTTGCCTTA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 17047 | GenBank | BAV50460 |
| Name | t4cp2_MLTONO_5558_ICEMlSymTONO |
UniProt ID | _ |
| Length | 676 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 676 a.a. Molecular weight: 75079.45 Da Isoelectric Point: 6.3712
>BAV50460.1 conjugal transfer coupling protein TraG [Mesorhizobium loti]
MTPTKLLIGQIAIVFAIVLLGVWAATQWCAHMLAFQEQLGAPWFVAAGWPIYEPWKLLEWWFQFDAYAPE
VFDKAGMLAGTSGFMGCAAAIAGSLWRARQRGLVTTYGSSRWAATREIEKAGLFQPEGVFLGKLKDRYLR
HDGPEHVMAFAPTRSGKGVGLVVPTLLSWSGSAVIHDIKGENWQLSSGWRSRFSHCLLFNPTDPRSARYN
PLLEVRKGSDEIRDVQNIADILVDPEGALERRNHWEKTSHSLLVGAILHVLYAEEEKTLARVATFLSDPQ
RSFAATLRRMMTTNHLGAANKPEVHPVVASAAREVLNKSENERSGVLSTAMSFLGLYRDPTVAAVTSDCD
WRIADLMDAEWPMSLYLVVPPSDISRTKPLVRLILNQIGRRLTERLEGDPKKSRKHQLLMMLDEFPALGR
LDFFETALAFMAGYGIRAYLIAQSLNQISKAYGDNNAILDNCHLRIAFSSNDERTAKRISDALGTATELR
SMRNYAGHRLAPWLSHVMVSRQETARPLLTPGEVMQLPPADELVLVSGLPPIRAKKLRYYEDHNFTERVL
PSPVLRDGPYADCPMSRPDDWTGQVRGVDRRLASDDESAGTALADEGGVQQQRHPGLPEEHTAVTQEPDQ
DDLSGPADDDSEAMADKRVMDRISTVAGAYGINEGRGDDKDIVPGF
MTPTKLLIGQIAIVFAIVLLGVWAATQWCAHMLAFQEQLGAPWFVAAGWPIYEPWKLLEWWFQFDAYAPE
VFDKAGMLAGTSGFMGCAAAIAGSLWRARQRGLVTTYGSSRWAATREIEKAGLFQPEGVFLGKLKDRYLR
HDGPEHVMAFAPTRSGKGVGLVVPTLLSWSGSAVIHDIKGENWQLSSGWRSRFSHCLLFNPTDPRSARYN
PLLEVRKGSDEIRDVQNIADILVDPEGALERRNHWEKTSHSLLVGAILHVLYAEEEKTLARVATFLSDPQ
RSFAATLRRMMTTNHLGAANKPEVHPVVASAAREVLNKSENERSGVLSTAMSFLGLYRDPTVAAVTSDCD
WRIADLMDAEWPMSLYLVVPPSDISRTKPLVRLILNQIGRRLTERLEGDPKKSRKHQLLMMLDEFPALGR
LDFFETALAFMAGYGIRAYLIAQSLNQISKAYGDNNAILDNCHLRIAFSSNDERTAKRISDALGTATELR
SMRNYAGHRLAPWLSHVMVSRQETARPLLTPGEVMQLPPADELVLVSGLPPIRAKKLRYYEDHNFTERVL
PSPVLRDGPYADCPMSRPDDWTGQVRGVDRRLASDDESAGTALADEGGVQQQRHPGLPEEHTAVTQEPDQ
DDLSGPADDDSEAMADKRVMDRISTVAGAYGINEGRGDDKDIVPGF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 17048 | GenBank | BAV50465 |
| Name | t4cp2_MLTONO_5563_ICEMlSymTONO |
UniProt ID | _ |
| Length | 816 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 816 a.a. Molecular weight: 90164.69 Da Isoelectric Point: 5.8719
>BAV50465.1 conjugal transfer ATPase TrbE [Mesorhizobium loti]
MLNLSEYRGKADRLADHLPWAALVAPGIVLNKDGSVQRTIRFRGPDLESSTEAELIGICARANNALRRLG
SGWTLFFEAERIEALGYPSSRFPDAASWLVDEERRAAFEGKVAHFESRYHLTLLSMPPPDAQARAESALV
DSHHSEGERDWRQEMARFRDETDRVLDLLSGFMPEVRLLDDAETLTYLHDTISTRRHPVAVPETPMYLDG
ILVDVPLTGGLEPMLGEQHLRTLTILGFPNLTRPGILDALNHQDFAYRWMTRFIPLGKTEATKTLTRLRR
QWFAKRKSIVAILREVVSNEPVPLVDSDADNKALDADEALQALGGDHVGFGYLTATVTVWDEDRQAAAEK
LRAVERIINGLGFTTIRESVNAVEAWLGSLPGHVYANVRQPLVHTLNLAHLMPLSSVWAGPAANEHLAEV
TGREAPPLLIAETSGSTPFRLSSHVEDVGHMLIVGPTGAGKSVLLALIALQFRRYAGAQVYVFDKGNSAR
AATLAMGGEHHVLGSDGALAFQPLRNIGDQGRRSWAAEWIAGLIAHENVTLTPEVKEAIWSALNSLATAP
AQERTLTGLSVLLQSNALKSALMPYTLDGPFGRLLDADDDRLALSDVQCFETEELMHSQSAVLPVLTYLF
HRLEERFDGRPTLLMLDEAWVYLDNPLFAARIREWLKVLRKKNVSVIFATQSLADIAGSAIAPAIIESCP
QRIFLPNDRAVEPQARTAYERFGLNDRQIELIARATPKRHYYLQSRCGNRLFELGLGPIALALCGASGPA
AQSLIDRVLSEHGQDSFVFRFLGACGLDWAAELLQQFPQPTKEQSS
MLNLSEYRGKADRLADHLPWAALVAPGIVLNKDGSVQRTIRFRGPDLESSTEAELIGICARANNALRRLG
SGWTLFFEAERIEALGYPSSRFPDAASWLVDEERRAAFEGKVAHFESRYHLTLLSMPPPDAQARAESALV
DSHHSEGERDWRQEMARFRDETDRVLDLLSGFMPEVRLLDDAETLTYLHDTISTRRHPVAVPETPMYLDG
ILVDVPLTGGLEPMLGEQHLRTLTILGFPNLTRPGILDALNHQDFAYRWMTRFIPLGKTEATKTLTRLRR
QWFAKRKSIVAILREVVSNEPVPLVDSDADNKALDADEALQALGGDHVGFGYLTATVTVWDEDRQAAAEK
LRAVERIINGLGFTTIRESVNAVEAWLGSLPGHVYANVRQPLVHTLNLAHLMPLSSVWAGPAANEHLAEV
TGREAPPLLIAETSGSTPFRLSSHVEDVGHMLIVGPTGAGKSVLLALIALQFRRYAGAQVYVFDKGNSAR
AATLAMGGEHHVLGSDGALAFQPLRNIGDQGRRSWAAEWIAGLIAHENVTLTPEVKEAIWSALNSLATAP
AQERTLTGLSVLLQSNALKSALMPYTLDGPFGRLLDADDDRLALSDVQCFETEELMHSQSAVLPVLTYLF
HRLEERFDGRPTLLMLDEAWVYLDNPLFAARIREWLKVLRKKNVSVIFATQSLADIAGSAIAPAIIESCP
QRIFLPNDRAVEPQARTAYERFGLNDRQIELIARATPKRHYYLQSRCGNRLFELGLGPIALALCGASGPA
AQSLIDRVLSEHGQDSFVFRFLGACGLDWAAELLQQFPQPTKEQSS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5740763..5749820
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MLTONO_5554 | 5736173..5736385 | - | 213 | BAV50456 | hypothetical protein | - |
| MLTONO_5555 | 5736838..5737167 | + | 330 | BAV50457 | Uncharacterized protein | - |
| MLTONO_5556 | 5737158..5737547 | + | 390 | BAV50458 | Putative uncharacterized protein msi033 | - |
| MLTONO_5557 | 5737522..5737821 | + | 300 | BAV50459 | Coronin | - |
| MLTONO_5558 | 5738204..5740234 | + | 2031 | BAV50460 | conjugal transfer coupling protein TraG | - |
| MLTONO_5559 | 5740353..5740766 | + | 414 | BAV50461 | CopG/DNA-binding domain-containing protein | - |
| MLTONO_5560 | 5740763..5741731 | + | 969 | BAV50462 | P-type conjugative transfer ATPase TrbB | virB11 |
| MLTONO_5561 | 5741743..5742051 | + | 309 | BAV50463 | conjugal transfer protein TrbC | virB2 |
| MLTONO_5562 | 5742051..5742323 | + | 273 | BAV50464 | conjugal transfer protein TrbD | virB3 |
| MLTONO_5563 | 5742317..5744767 | + | 2451 | BAV50465 | conjugal transfer ATPase TrbE | virb4 |
| MLTONO_5564 | 5744764..5745489 | + | 726 | BAV50466 | conjugal transfer protein TrbJ | virB5 |
| MLTONO_5565 | 5745521..5746843 | + | 1323 | BAV50467 | conjugal transfer protein TrbL | virB6 |
| MLTONO_5566 | 5746844..5747569 | + | 726 | BAV50468 | conjugal transfer protein TrbF | virB8 |
| MLTONO_5567 | 5747566..5748594 | + | 1029 | BAV50469 | conjugal transfer protein trbG | virB9 |
| MLTONO_5568 | 5748606..5749820 | + | 1215 | BAV50470 | conjugal transfer protein trbI | virB10 |
| MLTONO_5569 | 5749833..5750102 | + | 270 | BAV50471 | uncharacterized conserved small protein | - |
| MLTONO_5570 | 5750640..5751989 | - | 1350 | BAV50472 | coproporphyrinogen III oxidase | - |
| MLTONO_5571 | 5752249..5752902 | + | 654 | BAV50473 | cAMP-binding protein | - |
| MLTONO_5572 | 5752906..5753424 | - | 519 | BAV50474 | Uncharacterized protein | - |
Host bacterium
| ID | 397 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEMlSymTONO | GenBank | AP017605 |
| Element size | 7856088 bp | Coordinate of oriT [Strand] | 348729..348789 [-] |
| Host bacterium | Mesorhizobium loti | Coordinate of element | 5261386..5770004 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | nodF, nodE, nifA, noeL, fixT, nifZ, nifB, fixX, fixC, fixB, fixA, nifW, nifS, mLTONO_5203, nifQ, nifH, nifD, nifE, nifN, nifX, nifU, mlr6097, nodS, nodA, nodC, nodI, nodJ, nolO, nodB, nodD, mLTONO_5487, nolX, nolW, nopB, nolT, nolV, nopA, nodM, fixN, fixO, fixQ, fixP, fixG, fixH, fixI |
| Anti-CRISPR | AcrIIC1 |