Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200412
• Name: oriT_Tn6815
• Organism: Streptococcus mitis
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): LR828204 (2501..2633 [+], 133 nt)
• oriT length: 133 nt
• IRs (inverted repeats): 65..70, 83..88 (AAATCC..GGATTT) 6..12, 23..29 (ACCCCCC..GGGGGGT)
• Location of nic site: 75..76
• Conserved sequence flanking the nic site: TTTGGTTACA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 133 nt

>oriT_Tn6815
ACTTAACCCCCCGTTTCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_-_Tn6815

• ID: 14440
• GenBank: CAD0296864
• Name: Rep_trans_-_Tn6815
• UniProt ID: _
• Length: 401 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 401 a.a.
• Molecular weight: 47398.10 Da
• Isoelectric Point: 6.5091

>CAD0296864.1 hypothetical protein [Streptococcus mitis]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

Protein domains

Predicted by InterproScan.

• Helix-turn-helix (HTH_3) (15-53)

• Replication initiation factor (Rep_trans) (169-373)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (73-161)

Protein structure

No available structure.

Auxiliary protein

CAD0296881_Tn6815

• ID: 7888
• GenBank: CAD0296881
• Name: CAD0296881_Tn6815
• UniProt ID: _
• Length: 405 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 405 a.a.
• Molecular weight: 47021.92 Da
• Isoelectric Point: 9.7978

>CAD0296881.1 Transposase from transposon Tn916 [Streptococcus mitis]
MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKLVATDRVPAGKRDCISLREKIAELQKD
IHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSE
NGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYS
KNYDEILILLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQ
AFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFC
TNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA

Protein domains

Predicted by InterproScan.

• Phage integrase family (Phage_integrase) (190-383)

• DNA binding domain of tn916 integrase (Integrase_DNA) (1-71)

Protein structure

No available structure.

T4CP

ftsK_2_-_Tn6815

• ID: 16973
• GenBank: CAD0296863
• Name: ftsK_2_-_Tn6815
• UniProt ID: _
• Length: 461 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 461 a.a.
• Molecular weight: 53370.27 Da
• Isoelectric Point: 9.0687

>CAD0296863.1 DNA translocase FtsK [Streptococcus mitis]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (217-301)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 336..11746

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Locus_0	336..650	+	315	CAD0296861	hypothetical protein	orf23
Locus_1	669..1052	+	384	CAD0296862	hypothetical protein	orf23
Locus_2	1081..2466	+	1386	CAD0296863	DNA translocase FtsK	virb4
Locus_3	2644..3849	+	1206	CAD0296864	hypothetical protein	-
Locus_4	3892..4113	+	222	CAD0296865	hypothetical protein	orf19
Locus_5	4230..4727	+	498	CAD0296866	hypothetical protein	-
Locus_6	4702..5208	+	507	CAD0296867	hypothetical protein	orf17a
Locus_7	5192..7639	+	2448	CAD0296868	hypothetical protein	virb4
Locus_8	7708..9819	+	2112	CAD0296869	hypothetical protein	orf15
Locus_9	9816..10817	+	1002	CAD0296870	putative endopeptidase p60	orf14
Locus_10	10832..11746	+	915	CAD0296871	hypothetical protein	orf13
Locus_11	12123..14042	+	1920	CAD0296872	Tetracycline resistance protein TetM from transposon TnFO1	-
Locus_12	15149..15886	+	738	CAD0296873	rRNA adenine N-6-methyltransferase	-
Locus_13	15891..16022	+	132	CAD0296874	hypothetical protein	-

Host bacterium

• ID: 367
• Element type: ICE (Integrative and conjugative element)
• Element name: Tn6815
• GenBank: LR828204
• Element size: 23299 bp
• Coordinate of oriT [Strand]: 2501..2633 [+]
• Host bacterium: Streptococcus mitis
• Coordinate of element: 1..23299

Cargo genes

• Drug resistance gene: tet(M), erm(B)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -