Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200395 |
| Name | oriT_ICESsuYS146  |
| Organism | Streptococcus suis sequence |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | MK211816 (31555..31687 [-], 133 nt) |
| oriT length | 133 nt |
| IRs (inverted repeats) | 65..70, 83..88 (AAATCC..GGATTT) 6..12, 23..29 (ACCCCCC..GGGGGGT) |
| Location of nic site | 75..76 |
| Conserved sequence flanking the nic site |
TTTGGTTACA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 133 nt
>oriT_ICESsuYS146
ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC
ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14420 | GenBank | QID26145 |
| Name | Relaxase_YS146-GM000014_ICESsuYS146 |
UniProt ID | _ |
| Length | 621 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 621 a.a. Molecular weight: 73793.64 Da Isoelectric Point: 7.0198
>QID26145.1 relaxase [Streptococcus suis]
MVITKHYATHSRKYRKNLIRYILNPEKTAQLKLVSDFGMSNYLDFPSYEEMVKMYQANFLNNDGLYDSRN
DRQEKRQQKIHAHHLIQSFSPDDKLTPEEINRIGYETVKELTGGNFRFIVATHIDQSHIHNHILINSVDL
QSDKKLKWNYALERNLRMISDRISKEAGAKIITNRYSHQQYDVYRKTNHKFELKQRLYFLMEQSKDFEDF
LKKAEQLHLTIDFSSKHTTFLMTDRDQVKPIRGRQLNRREPYDESYFRQAFAKGAIEQRLHFLLPRVKTL
QELLEMAEELGLFIQLKQKNVTFTLTENDQKISLGHQQVSKKKLYDVSFFQDYFSERSDTDIELSDNIKE
DFEIFLAEQAEKLSTVESIVSDYETFSENRKAVHEFEVELAPHQVDKEVEGGIFIKVAFGVKKEGLIFIP
NYQLDEIEKEEEKRFKIFLREITSYFIYNKESSDKNRYMKGRDLIRQLTQDNKSLPYRRRPNLKELQEKI
TEVNLLIELNVTNRAYTDIKDELVAEIATYDVSMSELNEKTATLNKIAEVLVNLKSHDIERRRLARYEFS
KLNLTESVTLEQIEREISNTQDQLSKLLDNYEESVRKLETFVAVLNRTAPRTRKDYDKELY
MVITKHYATHSRKYRKNLIRYILNPEKTAQLKLVSDFGMSNYLDFPSYEEMVKMYQANFLNNDGLYDSRN
DRQEKRQQKIHAHHLIQSFSPDDKLTPEEINRIGYETVKELTGGNFRFIVATHIDQSHIHNHILINSVDL
QSDKKLKWNYALERNLRMISDRISKEAGAKIITNRYSHQQYDVYRKTNHKFELKQRLYFLMEQSKDFEDF
LKKAEQLHLTIDFSSKHTTFLMTDRDQVKPIRGRQLNRREPYDESYFRQAFAKGAIEQRLHFLLPRVKTL
QELLEMAEELGLFIQLKQKNVTFTLTENDQKISLGHQQVSKKKLYDVSFFQDYFSERSDTDIELSDNIKE
DFEIFLAEQAEKLSTVESIVSDYETFSENRKAVHEFEVELAPHQVDKEVEGGIFIKVAFGVKKEGLIFIP
NYQLDEIEKEEEKRFKIFLREITSYFIYNKESSDKNRYMKGRDLIRQLTQDNKSLPYRRRPNLKELQEKI
TEVNLLIELNVTNRAYTDIKDELVAEIATYDVSMSELNEKTATLNKIAEVLVNLKSHDIERRRLARYEFS
KLNLTESVTLEQIEREISNTQDQLSKLLDNYEESVRKLETFVAVLNRTAPRTRKDYDKELY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 14421 | GenBank | QID26162 |
| Name | Rep_trans_YS146-GM000031_ICESsuYS146 |
UniProt ID | _ |
| Length | 401 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 401 a.a. Molecular weight: 47398.10 Da Isoelectric Point: 6.5091
>QID26162.1 transcriptional regulator, Cro/CI family [Streptococcus suis]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 7887 | GenBank | QID26149 |
| Name | QID26149_ICESsuYS146 |
UniProt ID | _ |
| Length | 405 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 405 a.a. Molecular weight: 47021.92 Da Isoelectric Point: 9.7978
>QID26149.1 site-specific recombinase [Streptococcus suis]
MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKLVATDRVPAGKRDCISLREKIAELQKD
IHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSE
NGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYS
KNYDEILILLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQ
AFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFC
TNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA
MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKLVATDRVPAGKRDCISLREKIAELQKD
IHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSE
NGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYS
KNYDEILILLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQ
AFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFC
TNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16942 | GenBank | QID26163 |
| Name | tcpA_YS146-GM000032_ICESsuYS146 |
UniProt ID | _ |
| Length | 461 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 461 a.a. Molecular weight: 53370.27 Da Isoelectric Point: 9.0687
>QID26163.1 Putative cell-division FtsK/SpoIIIE-family protein Tn5397 [Streptococcus suis]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16943 | GenBank | QID26193 |
| Name | t4cp2_YS146-GM000065_ICESsuYS146 |
UniProt ID | _ |
| Length | 598 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 598 a.a. Molecular weight: 68616.30 Da Isoelectric Point: 9.3978
>QID26193.1 conjugal transfer protein TraG [Streptococcus suis]
MYSRQKAFVFGLLGLAFGYFCHRLILLYDSLTNAPPMERFAYLLGEGINQVFNPLWQFTFTQKSFLAFIL
GVLTTMLVYLYVSTGQKVYREGEEYGSARFGTSKEKRFFYSKNPFNDTILARDVRLTLLEKKKPQFDRNK
NLIVIGGSGAGKTFRFVKPNLIQLNCSNIVVDPKDHLAEKSGKLFLENGYQVKVLDLVNMTNSDGFNPFR
YVETENDLNRMLTVYFNNTRGSGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRGRYPA
FSEIGKLIKLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNSVIAVTTAKFALFNIKSVIDLT
QRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALFFSTVFSTLTRQADVDFKGQLPIHVRSYLDEFSNVG
EIPDFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNDEETFKFMSGLLGKQ
TIDVRSTSRSFGQTGSSSTSHQKIARDLMTADEVGNMKRDECLVRIAGVPVFRTKKYFPLKHKNWKWLAD
KETDVRWWHYHINPLTAEEEVDLSGHKIRDLSTETTLH
MYSRQKAFVFGLLGLAFGYFCHRLILLYDSLTNAPPMERFAYLLGEGINQVFNPLWQFTFTQKSFLAFIL
GVLTTMLVYLYVSTGQKVYREGEEYGSARFGTSKEKRFFYSKNPFNDTILARDVRLTLLEKKKPQFDRNK
NLIVIGGSGAGKTFRFVKPNLIQLNCSNIVVDPKDHLAEKSGKLFLENGYQVKVLDLVNMTNSDGFNPFR
YVETENDLNRMLTVYFNNTRGSGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRGRYPA
FSEIGKLIKLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNSVIAVTTAKFALFNIKSVIDLT
QRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALFFSTVFSTLTRQADVDFKGQLPIHVRSYLDEFSNVG
EIPDFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNDEETFKFMSGLLGKQ
TIDVRSTSRSFGQTGSSSTSHQKIARDLMTADEVGNMKRDECLVRIAGVPVFRTKKYFPLKHKNWKWLAD
KETDVRWWHYHINPLTAEEEVDLSGHKIRDLSTETTLH
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 22442..75296
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| YS146-GM000019 | 17629..17832 | - | 204 | QID26150 | hypothetical protein | - |
| YS146-GM000020 | 18293..18523 | - | 231 | QID26151 | hypothetical protein | - |
| YS146-GM000021 | 18520..18942 | - | 423 | QID26152 | hypothetical protein | - |
| YS146-GM000022 | 19447..19800 | + | 354 | QID26153 | DNA-binding transcriptional regulator, XRE-family HTH domain | - |
| YS146-GM000023 | 20146..22080 | - | 1935 | QID26154 | TetM | - |
| YS146-GM000024 | 22442..23374 | - | 933 | QID26155 | hypothetical protein | orf13 |
| YS146-GM000025 | 23371..24372 | - | 1002 | QID26156 | Tn916 NLP/P60 family protein | orf14 |
| YS146-GM000026 | 24369..26480 | - | 2112 | QID26157 | membrane protein | orf15 |
| YS146-GM000027 | 26549..28996 | - | 2448 | QID26158 | Conjugative transposon ATP/GTP-binding protein (plasmid) | virb4 |
| YS146-GM000028 | 28980..29486 | - | 507 | QID26159 | Conjugative transposon membrane protein, TcpE family (plasmid) | orf17a |
| YS146-GM000029 | 29461..29958 | - | 498 | QID26160 | Antirestriction (ArdA) protein/ Conjugative transposon protein (plasmid) | - |
| YS146-GM000030 | 30075..30296 | - | 222 | QID26161 | Putative conjugative transposon protein Tn5397 | orf19 |
| YS146-GM000031 | 30339..31544 | - | 1206 | QID26162 | transcriptional regulator, Cro/CI family | - |
| YS146-GM000032 | 31722..33107 | - | 1386 | QID26163 | Putative cell-division FtsK/SpoIIIE-family protein Tn5397 | virb4 |
| YS146-GM000033 | 33136..33519 | - | 384 | QID26164 | putative integrative and conjugative element protein | orf23 |
| YS146-GM000035 | 34214..34513 | + | 300 | QID26165 | hypothetical protein | - |
| YS146-GM000036 | 34608..36425 | - | 1818 | QID26166 | DNA helicase | - |
| YS146-GM000037 | 36412..38499 | - | 2088 | QID26167 | ATP-dependent endonuclease | - |
| YS146-GM000038 | 38496..39272 | - | 777 | QID26168 | Signal recognition particle GTPase | - |
| YS146-GM000039 | 39272..39748 | - | 477 | QID26169 | transcriptional regulator | - |
| YS146-GM000040 | 39818..40168 | - | 351 | QID26170 | hypothetical protein | - |
| YS146-GM000041 | 40158..40547 | - | 390 | QID26171 | hypothetical protein | gbs1346 |
| YS146-GM000042 | 40544..40771 | - | 228 | QID26172 | hypothetical protein | gbs1347 |
| YS146-GM000043 | 40825..41901 | - | 1077 | QID26173 | hypothetical protein | - |
| YS146-GM000044 | 41940..42578 | - | 639 | QID26174 | parvulin-like peptidyl-prolyl isomerase | prgL |
| YS146-GM000045 | 42712..44946 | + | 2235 | QID26175 | hypothetical protein | - |
| YS146-GM000046 | 44932..46194 | - | 1263 | QID26176 | serine hydroxymethyltransferase | - |
| YS146-GM000047 | 46285..47172 | + | 888 | QID26177 | integrase | - |
| YS146-GM000048 | 47217..47516 | - | 300 | QID26178 | hypothetical protein | gbs1350 |
| YS146-GM000049 | 47530..47829 | - | 300 | QID26179 | hypothetical protein | - |
| YS146-GM000050 | 47900..54724 | - | 6825 | QID26180 | hypothetical protein | - |
| YS146-GM000051 | 54776..55327 | - | 552 | QID26181 | hypothetical protein | gbs1354 |
| YS146-GM000052 | 55311..55502 | - | 192 | QID26182 | calcium-binding protein | - |
| YS146-GM000053 | 55503..59240 | - | 3738 | QID26183 | LPXTG-motif cell wall anchor domain protein | prgB |
| YS146-GM000054 | 59638..60753 | - | 1116 | QID26184 | agglutinin receptor | prgB |
| YS146-GM000055 | 61054..62448 | - | 1395 | QID26185 | hypothetical protein | - |
| YS146-GM000056 | 62455..63297 | - | 843 | QID26186 | abortive infection protein | - |
| YS146-GM000057 | 63294..63887 | - | 594 | QID26187 | transcriptional regulator | - |
| YS146-GM000058 | 64009..66810 | - | 2802 | QID26188 | amidase | prgK |
| YS146-GM000060 | 67779..69485 | - | 1707 | QID26189 | putative reverse transcriptase | - |
| YS146-GM000062 | 71431..71784 | - | 354 | QID26190 | PrgI family protein | prgIc |
| YS146-GM000063 | 71846..72694 | - | 849 | QID26191 | conjugal transfer protein TrbL | prgHb |
| YS146-GM000064 | 72719..72961 | - | 243 | QID26192 | hypothetical protein | prgF |
| YS146-GM000065 | 72979..74775 | - | 1797 | QID26193 | conjugal transfer protein TraG | virb4 |
| YS146-GM000066 | 74775..75296 | - | 522 | QID26194 | hypothetical protein | gbs1365 |
| YS146-GM000067 | 75341..75925 | - | 585 | QID26195 | CAAX amino protease | - |
| YS146-GM000068 | 75928..76161 | - | 234 | QID26196 | putative conjugative element protein | - |
| YS146-GM000069 | 76170..76553 | - | 384 | QID26197 | arsenate reductase | - |
| YS146-GM000070 | 77111..77929 | - | 819 | QID26198 | replication initiator protein A domain protein | - |
| YS146-GM000071 | 77926..78099 | - | 174 | QID26199 | hypothetical protein | - |
| YS146-GM000072 | 78160..78279 | - | 120 | QID26200 | hypothetical protein | - |
| YS146-GM000073 | 78299..78664 | - | 366 | QID26201 | LSU ribosomal protein L7/L12 (P1/P2) rplL | - |
Host bacterium
| ID | 351 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICESsuYS146 | GenBank | MK211816 |
| Element size | 78664 bp | Coordinate of oriT [Strand] | 31555..31687 [-] |
| Host bacterium | Streptococcus suis sequence | Coordinate of element | 1..78664 |
Cargo genes
| Drug resistance gene | tet(M) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |