Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200394
• Name: oriT2_ICESsuSC317
• Organism: Streptococcus suis strain SC317
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): MK359989 ( 31681..31715 [-], 35 nt)
• oriT length: 35 nt
• IRs (inverted repeats): 5..11, 15..21 (ACTTTGC..GCAAAGT)
• Location of nic site: 29..30
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 35 nt

>oriT2_ICESsuSC317
CACGACTTTGCGAAGCAAAGTGTAGTGTGTTATAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_-_ICESsuSC317

• ID: 14418
• GenBank: QEM40798
• Name: Relaxase_-_ICESsuSC317
• UniProt ID: _
• Length: 627 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 627 a.a.
• Molecular weight: 74663.90 Da
• Isoelectric Point: 7.1885

>QEM40798.1 relaxase [Streptococcus suis]
MVVTKHFAVHGNKYRKSLIKYILNPAKTDQLKLVSDFGMSNYLDFPNYEEIVEMYQANFVNNDRLYDSRN
DRQQIKQNKIHAHHLIQSFSPEDNLTPEEINRIGYETIKELTSGNFRFIVTTHTDRLHVHNHILINSVDL
NSHKKLKWDYAQERNLRMISDRLSKEAGAKIITPNRYSHEKFVTYRKSNHKFELKQRLYFLMENSKDFDD
FLSKAEALNVQIDFSRKYARFLMTDMPMKRVIRGKQLDKRQPYTEEYFREHFAKRAIEQCLDFLLPRVRD
LSQLLEFARELNLAMSLKQKNVAFTLTKNSQSITVNNQKVSSKNLYDVQFFETYFEKRGEVPDIDQSQLI
SDFEGYCEEQDKEKLAYEDLQEAYQAFKEKRDQVQEFEVVLADHQIDKLVKDGLFIRMNYGVKKDGLVFI
PNRHLDIKETESGKHYHAFIRETAQFFIYNKEASELNRYMRGRELIRQLTNDSQTISKRKRPTIDTLKKK
IEEINLLIELGTENKSYQDIKDEIIKDIAQLDLTITEIQEHIDHLNKVAEVLLNLDNNDIENRRLAKYDY
AKMNLTAAIKLEQVEKEIEIYQIKLDKSIDDYEYLIRRLEKFVKILNEIDSSKDELKFEHSEKNVKE

Protein domains

Predicted by InterproScan.

• Lantibiotic streptin immunity protein (Relaxase_C) (518-608)

• Relaxase/Mobilisation nuclease domain (Relaxase) (12-265)

Protein structure

No available structure.

mob_-_ICESsuSC317

• ID: 14419
• GenBank: QEM40802
• Name: mob_-_ICESsuSC317
• UniProt ID: _
• Length: 365 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 365 a.a.
• Molecular weight: 42061.21 Da
• Isoelectric Point: 10.1244

>QEM40802.1 plasmid recombination protein Mob family [Streptococcus suis]
MTVMQGVRVTVAAVGRGYPKTAVTTVTAVTPKDGHPPKERRNPPMPYAILRFQKRKAGGVAACERHNERK
KEAYKSNPDIDMERSKNNYHLIAPPKYTYKKEINRMVAEAGCRTRKDSVMMVETLITASPEFMNQLPPEE
QKAYFQTALDFISERVGKQNILSAVVHMDERTPHMHLCFVPITPDNKLSAKAILGNQKSLSEWQTAYHER
MSSRWNQLERGQSSMETKRKHVPTWLYKLGGRLDKQYEEIVSALSDINAFNAGKKRDKALDLLSAWLPDV
EKFSKEIGKQQAYIDSLKERIGQESDYAGRMRDEKYEQELKVQKANQKIFELQRTNEQMGRLLSKIPPEV
LEELQKNHRSRAKER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (45-238)

Protein structure

No available structure.

T4CP

traG_-_ICESsuSC317

• ID: 16940
• GenBank: QEM40813
• Name: traG_-_ICESsuSC317
• UniProt ID: _
• Length: 605 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 605 a.a.
• Molecular weight: 69401.21 Da
• Isoelectric Point: 9.1895

>QEM40813.1 conjugal transfer protein [Streptococcus suis]
MYSRRKALVFGLLGLAFGYFCHRLTLLYDSLTNAPPMERFAYLLGEGLNQVFNPLWLFSFTQKSLLAFIL
GVLTMTLVYLYVSTGQKVYREGEEYGSARFGTSKEKWNFYSKNPLNDTILARDVRLTLLEKKKPQFDRNK
NLIVIGGSGAGKTFRFVKPNLIQLNCSNIVVDPKDHLAEKTGKLFLENGYQVKVLDLVNMTNSDGFNPFR
YVETENDLNRMLTVYFNNTKGNGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRGRYPA
FSEIGKLIKLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNI
QSVIDLTQRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALFFSTVFATLTRQADVDFKGQLPIHVRSYL
DEFANVGEIPDFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNDEETFKFM
SDLLGKQTIDVRSTSRSFGQTGSSSTSHQKIARDLMTADEVGNMKRDECLVRIAGVPVFRTKKYFPLKHK
NWKWLADKETDVRWWHYHINPLTAEEEVDLSGHKIRDLSTETTLH

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (129-555)

Protein structure

No available structure.

traG_-_ICESsuSC317

• ID: 16940
• GenBank: QEM40813
• Name: traG_-_ICESsuSC317
• UniProt ID: _
• Length: 605 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 605 a.a.
• Molecular weight: 69401.21 Da
• Isoelectric Point: 9.1895

>QEM40813.1 conjugal transfer protein [Streptococcus suis]
MYSRRKALVFGLLGLAFGYFCHRLTLLYDSLTNAPPMERFAYLLGEGLNQVFNPLWLFSFTQKSLLAFIL
GVLTMTLVYLYVSTGQKVYREGEEYGSARFGTSKEKWNFYSKNPLNDTILARDVRLTLLEKKKPQFDRNK
NLIVIGGSGAGKTFRFVKPNLIQLNCSNIVVDPKDHLAEKTGKLFLENGYQVKVLDLVNMTNSDGFNPFR
YVETENDLNRMLTVYFNNTKGNGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRGRYPA
FSEIGKLIKLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNI
QSVIDLTQRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALFFSTVFATLTRQADVDFKGQLPIHVRSYL
DEFANVGEIPDFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNDEETFKFM
SDLLGKQTIDVRSTSRSFGQTGSSSTSHQKIARDLMTADEVGNMKRDECLVRIAGVPVFRTKKYFPLKHK
NWKWLADKETDVRWWHYHINPLTAEEEVDLSGHKIRDLSTETTLH

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (129-555)

Protein structure

No available structure.

Host bacterium

• ID: 350
• Element type: ICE (Integrative and conjugative element)
• Element name: ICESsuSC317
• GenBank: MK359989
• Element size: 106789 bp
• Coordinate of oriT [Strand]: 3136..3158 [-]; 31681..31715 [-]
• Host bacterium: Streptococcus suis strain SC317
• Coordinate of element: 1..106789

Cargo genes

• Drug resistance gene: tet(L), ant(6)-Ia, tet(O), optrA
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21