Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200391
• Name: oriT_ICESpsSUT-286-2
• Organism: Streptococcus parasuis SUT-286
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): AP024276 (39110..39242 [-], 133 nt)
• oriT length: 133 nt
• IRs (inverted repeats): 65..70, 83..88 (AAATCC..GGATTT) 6..12, 23..29 (ACCCCCC..GGGGGGT)
• Location of nic site: 75..76
• Conserved sequence flanking the nic site: TTTGGTTACA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 133 nt

>oriT_ICESpsSUT-286-2
ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_SUT286_17590_ICESpsSUT-286-2

• ID: 14416
• GenBank: BCP60433
• Name: Rep_trans_SUT286_17590_ICESpsSUT-286-2
• UniProt ID: _
• Length: 371 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 371 a.a.
• Molecular weight: 43729.94 Da
• Isoelectric Point: 6.6982

>BCP60433.1 Cro/Cl family transcriptional regulator [Streptococcus parasuis]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVAGGVM
KRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEEKECMGNTLYIGSLQSEVYFCIY
EKDYEQYKKNDIPIEDAEVKKRFEIRLKNERAYYAVRDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSD
WKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTD
RHKQILKQQSVKEQDVITTKK

Protein domains

Predicted by InterproScan.

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (73-135)

• Replication initiation factor (Rep_trans) (139-343)

• Helix-turn-helix (HTH_3) (15-53)

Protein structure

No available structure.

Auxiliary protein

BCP60420_ICESpsSUT-286-2

• ID: 7886
• GenBank: BCP60420
• Name: BCP60420_ICESpsSUT-286-2
• UniProt ID: _
• Length: 405 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 405 a.a.
• Molecular weight: 47021.92 Da
• Isoelectric Point: 9.7978

>BCP60420.1 integrase family protein [Streptococcus parasuis]
MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKLVATDRVPAGKRDCISLREKIAELQKD
IHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSE
NGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYS
KNYDEILILLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQ
AFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFC
TNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA

Protein domains

Predicted by InterproScan.

• Phage integrase family (Phage_integrase) (190-383)

• DNA binding domain of tn916 integrase (Integrase_DNA) (1-71)

Protein structure

No available structure.

T4CP

ftsK_3_SUT286_17600_ICESpsSUT-286-2

• ID: 16938
• GenBank: BCP60434
• Name: ftsK_3_SUT286_17600_ICESpsSUT-286-2
• UniProt ID: _
• Length: 461 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 461 a.a.
• Molecular weight: 53370.27 Da
• Isoelectric Point: 9.0687

>BCP60434.1 cell division protein FtsK [Streptococcus parasuis]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLIYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (217-301)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1789239..1800559

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SUT286_17470	1784426..1784629	-	204	BCP60421	transposase	-
SUT286_17480	1785090..1785320	-	231	BCP60422	hypothetical protein	-
SUT286_17490	1785317..1785739	-	423	BCP60423	hypothetical protein	-
SUT286_17500	1786244..1786597	+	354	BCP60424	transcriptional regulator	-
SUT286_17510	1786943..1788862	-	1920	BCP60425	tetracycline resistance protein TetM	-
SUT286_17520	1789239..1790171	-	933	BCP60426	transposase	orf13
SUT286_17530	1790168..1791169	-	1002	BCP60427	NlpC/P60 family hydrolase	orf14
SUT286_17540	1791166..1793343	-	2178	BCP60428	transposase	orf15
SUT286_17550	1793346..1795793	-	2448	BCP60429	ATP/GTP-binding protein	virb4
SUT286_17560	1795777..1796169	-	393	BCP60430	membrane protein	orf17a
SUT286_17570	1796258..1796755	-	498	BCP60431	antirestriction protein ArdA	-
SUT286_17580	1796872..1797093	-	222	BCP60432	conjugal transfer protein	orf19
SUT286_17590	1797136..1798251	-	1116	BCP60433	Cro/Cl family transcriptional regulator	-
SUT286_17600	1798429..1799814	-	1386	BCP60434	cell division protein FtsK	virb4
SUT286_17610	1799843..1800229	-	387	BCP60435	transposase	orf23
SUT286_17620	1800245..1800559	-	315	BCP60436	transposase	orf23
SUT286_17630	1801488..1802756	-	1269	BCP60437	MATE family efflux transporter	-
SUT286_17640	1802949..1804352	-	1404	BCP60438	threonine synthase	-
SUT286_17650	1804507..1805412	-	906	BCP60439	oligopeptide ABC transporter permease protein	-

Host bacterium

• ID: 348
• Element type: ICE (Integrative and conjugative element)
• Element name: ICESpsSUT-286-2
• GenBank: AP024276
• Element size: 2197342 bp
• Coordinate of oriT [Strand]: 39110..39242 [-]
• Host bacterium: Streptococcus parasuis SUT-286
• Coordinate of element: 1759153..1810570

Cargo genes

• Drug resistance gene: tet(M)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -