Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200390 |
| Name | oriT_ICESpsH35閳?  |
| Organism | Streptococcus parasuis strain H35 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP076721 (22968..23100 [+], 133 nt) |
| oriT length | 133 nt |
| IRs (inverted repeats) | 65..70, 83..88 (AAATCC..GGATTT) 6..12, 23..29 (ACCCCCC..GGGGGGT) |
| Location of nic site | 75..76 |
| Conserved sequence flanking the nic site |
TTTGGTTACA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 133 nt
>oriT_ICESpsH35閳?
ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC
ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14415 | GenBank | QWV86435 |
| Name | Rep_trans_KQ224_10560_ICESpsH35閳? |
UniProt ID | _ |
| Length | 401 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 401 a.a. Molecular weight: 47398.10 Da Isoelectric Point: 6.5091
>QWV86435.1 replication initiation factor domain-containing protein [Streptococcus parasuis]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 7885 | GenBank | QWV86449 |
| Name | QWV86449_ICESpsH35閳? |
UniProt ID | _ |
| Length | 405 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 405 a.a. Molecular weight: 47021.92 Da Isoelectric Point: 9.7978
>QWV86449.1 tyrosine-type recombinase/integrase [Streptococcus parasuis]
MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKLVATDRVPAGKRDCISLREKIAELQKD
IHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSE
NGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYS
KNYDEILILLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQ
AFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFC
TNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA
MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKLVATDRVPAGKRDCISLREKIAELQKD
IHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSE
NGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYS
KNYDEILILLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQ
AFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFC
TNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16937 | GenBank | QWV86433 |
| Name | tcpA_KQ224_10550_ICESpsH35閳? |
UniProt ID | _ |
| Length | 461 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 461 a.a. Molecular weight: 53370.27 Da Isoelectric Point: 9.0687
>QWV86433.1 DUF87 domain-containing protein [Streptococcus parasuis]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2039762..2051171
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KQ224_10515 | 2035543..2036409 | - | 867 | QWV86426 | YitT family protein | - |
| KQ224_10520 | 2036552..2037190 | + | 639 | QWV86427 | dTMP kinase | - |
| KQ224_10525 | 2037187..2038068 | + | 882 | QWV86428 | DNA polymerase III subunit delta' | - |
| KQ224_10530 | 2038092..2038409 | + | 318 | QWV86429 | DNA replication initiation control protein YabA | - |
| KQ224_10535 | 2038411..2039274 | + | 864 | QWV86430 | 16S rRNA (cytidine(1402)-2'-O)-methyltransferase | - |
| KQ224_10540 | 2039762..2040076 | + | 315 | QWV86431 | YdcP family protein | orf23 |
| KQ224_10545 | 2040092..2040478 | + | 387 | QWV86432 | YdcP family protein | orf23 |
| KQ224_10550 | 2040507..2041892 | + | 1386 | QWV86433 | DUF87 domain-containing protein | virb4 |
| KQ224_10555 | 2041895..2042047 | + | 153 | QWV86434 | conjugal transfer protein | - |
| KQ224_10560 | 2042070..2043275 | + | 1206 | QWV86435 | replication initiation factor domain-containing protein | - |
| KQ224_10565 | 2043318..2043539 | + | 222 | QWV86436 | hypothetical protein | orf19 |
| KQ224_10570 | 2043656..2044153 | + | 498 | QWV86437 | antirestriction protein ArdA | - |
| KQ224_10575 | 2044128..2044634 | + | 507 | QWV86438 | conjugal transfer protein | orf17a |
| KQ224_10580 | 2044618..2047065 | + | 2448 | QWV86439 | ATP-binding protein | virb4 |
| KQ224_10585 | 2047179..2049332 | + | 2154 | QWV87630 | YtxH domain-containing protein | orf15 |
| KQ224_10590 | 2049241..2050242 | + | 1002 | QWV86440 | bifunctional lysozyme/C40 family peptidase | orf14 |
| KQ224_10595 | 2050239..2051171 | + | 933 | QWV86441 | conjugal transfer protein | orf13 |
| KQ224_10600 | 2051416..2051532 | + | 117 | QWV86442 | tetracycline resistance determinant leader peptide | - |
| KQ224_10605 | 2051548..2053467 | + | 1920 | QWV86443 | tetracycline resistance ribosomal protection protein Tet(M) | - |
| KQ224_10610 | 2053586..2053753 | + | 168 | QWV86444 | cysteine-rich KTR domain-containing protein | - |
| KQ224_10615 | 2053813..2054166 | - | 354 | QWV86445 | helix-turn-helix transcriptional regulator | - |
| KQ224_10620 | 2054671..2055093 | + | 423 | QWV86446 | sigma-70 family RNA polymerase sigma factor | - |
| KQ224_10625 | 2055090..2055320 | + | 231 | QWV86447 | helix-turn-helix domain-containing protein | - |
| KQ224_10630 | 2055781..2055984 | + | 204 | QWV86448 | excisionase | - |
Host bacterium
| ID | 347 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICESpsH35閳? | GenBank | CP076721 |
| Element size | 2186318 bp | Coordinate of oriT [Strand] | 22968..23100 [+] |
| Host bacterium | Streptococcus parasuis strain H35 | Coordinate of element | 2018960..2081277 |
Cargo genes
| Drug resistance gene | tet(M) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |