Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200383 |
| Name | oriT_ICEPmiIre01  |
| Organism | Proteus mirabilis strain Ire01 integrative and |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | MN520463 (4644..4942 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 45..50, 52..57 (CAAACG..CGTTTG) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEPmiIre01
GCTCTGTTTGGCGGTGGATGACTGAGCCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTCAGGCATTTATATGTGAAATCGGGCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTAGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGTGGATGACTGAGCCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTCAGGCATTTATATGTGAAATCGGGCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTAGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14409 | GenBank | QKR72253 |
| Name | TraI_2_-_ICEPmiIre01 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79845.91 Da Isoelectric Point: 5.2884
>QKR72253.1 conjugative transfer protein TraI relaxase [Proteus mirabilis]
MFKNLFFQTKALPELSSQLDADIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDLEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWLRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSSVRLFSKSEWEATRQTQAEPQSRSSEHSDL
PEASSSIEHRKSDESPSTNSSEQDDELRLASDVNNPQATENAPGDGCEKPNNSYDGAISNNVNQHDAEAL
NLPESLAWLPEASSALVVVGEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTITTKDGV
QEQGLLLKISISKGLTALIDLPKQDAEPAAAIQNEEASPRPSRTETTNVQAKEPATRTERKQKPIAANAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAKR
MFKNLFFQTKALPELSSQLDADIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDLEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWLRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSSVRLFSKSEWEATRQTQAEPQSRSSEHSDL
PEASSSIEHRKSDESPSTNSSEQDDELRLASDVNNPQATENAPGDGCEKPNNSYDGAISNNVNQHDAEAL
NLPESLAWLPEASSALVVVGEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTITTKDGV
QEQGLLLKISISKGLTALIDLPKQDAEPAAAIQNEEASPRPSRTETTNVQAKEPATRTERKQKPIAANAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAKR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16927 | GenBank | QKR72303 |
| Name | t4cp2_-_ICEPmiIre01 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67111.98 Da Isoelectric Point: 6.5130
>QKR72303.1 IncF plasmid conjugative transfer protein TraD [Proteus mirabilis]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLSELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYLPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAV
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLSELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYLPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16927 | GenBank | QKR72303 |
| Name | t4cp2_-_ICEPmiIre01 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67111.98 Da Isoelectric Point: 6.5130
>QKR72303.1 IncF plasmid conjugative transfer protein TraD [Proteus mirabilis]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLSELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYLPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAV
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLSELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYLPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 28680..52458
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_22 | 26481..28631 | + | 2151 | QKR72253 | conjugative transfer protein TraI relaxase | - |
| Locus_23 | 28680..30500 | + | 1821 | QKR72303 | IncF plasmid conjugative transfer protein TraD | virb4 |
| Locus_24 | 30510..31070 | + | 561 | QKR72254 | conjugative transfer protein 234 | - |
| Locus_25 | 31057..31692 | + | 636 | QKR72255 | conjugative transfer protein s043 | tfc7 |
| Locus_26 | 31719..32306 | - | 588 | QKR72256 | hypothetical protein | - |
| Locus_27 | 32320..32559 | - | 240 | QKR72257 | hypothetical protein | - |
| Locus_28 | 32595..32876 | + | 282 | QKR72304 | IncF plasmid conjugative transfer pilus assembly protein TraL | traL |
| Locus_29 | 32873..33499 | + | 627 | QKR72305 | IncF plasmid conjugative transfer pilus assembly protein TraE | traE |
| Locus_30 | 33480..34379 | + | 900 | QKR72258 | IncF plasmid conjugative pilus assembly protein TraK | traK |
| Locus_31 | 34382..35671 | + | 1290 | QKR72259 | IncF plasmid conjugative transfer pilus assembly protein TraB | traB |
| Locus_32 | 35746..36318 | + | 573 | QKR72260 | conjugative transfer protein TraV | traV |
| Locus_33 | 36315..36701 | + | 387 | QKR72261 | conjugative transfer protein TraA | - |
| Locus_34 | 36880..37713 | + | 834 | QKR72262 | transcriptional regulator AbiEi 3 antitoxin Type IV TA system | - |
| Locus_35 | 37706..38644 | + | 939 | QKR72263 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | - |
| Locus_36 | 38776..39468 | + | 693 | QKR72264 | thiol:disulfide interchange protein DsbC | trbB |
| Locus_37 | 39468..41867 | + | 2400 | QKR72265 | IncF plasmid conjugative transfer pilus assembly protein TraC | virb4 |
| Locus_38 | 41860..42207 | + | 348 | QKR72266 | conjugative transfer protein 345 | - |
| Locus_39 | 42191..42703 | + | 513 | QKR72267 | conjugative signal peptidase TrhF | - |
| Locus_40 | 42714..43838 | + | 1125 | QKR72268 | IncF plasmid conjugative transfer pilus assembly protein TraW | traW |
| Locus_41 | 43870..44850 | + | 981 | QKR72269 | IncF plasmid conjugative transfer pilus assembly protein TraU | traU |
| Locus_42 | 44853..48545 | + | 3693 | QKR72270 | IncF plasmid conjugative transfer protein TraN | traN |
| Locus_43 | 49004..50422 | + | 1419 | QKR72271 | hypothetical protein | - |
| Locus_44 | 50419..52458 | + | 2040 | QKR72272 | bipolar DNA helicase HerA | virb4 |
| Locus_45 | 52867..53550 | - | 684 | QKR72273 | endonuclease I precursor | - |
| Locus_46 | 53659..54261 | - | 603 | QKR72274 | hypothetical protein | - |
| Locus_47 | 54627..54953 | + | 327 | QKR72275 | hypothetical protein | - |
| Locus_48 | 54969..55388 | + | 420 | QKR72276 | single-stranded DNA-binding protein | - |
| Locus_49 | 55468..56286 | + | 819 | QKR72277 | phage recombination protein bet | - |
| Locus_50 | 56370..56513 | + | 144 | QKR72278 | hypothetical protein | - |
Host bacterium
| ID | 340 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEPmiIre01 | GenBank | MN520463 |
| Element size | 81300 bp | Coordinate of oriT [Strand] | 4644..4942 [+] |
| Host bacterium | Proteus mirabilis strain Ire01 integrative and | Coordinate of element | 1..81300 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |