Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200381 |
| Name | oriT_ICEPmiChn-TQ12  |
| Organism | Proteus terrae subsp. cibarius strain TQ12 ICEPmiChn-TQ12 mobile element |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | ON390822 (29801..30099 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 234..242, 254..262 (AAAGCCAAA..TTTGGCTTT) 45..50, 52..57 (CAAACG..CGTTTG) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEPmiChn-TQ12
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14407 | GenBank | UVX23107 |
| Name | TraI_2_-_ICEPmiChn-TQ12 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79894.02 Da Isoelectric Point: 5.0500
>UVX23107.1 Conjugative transfer protein TraI relaxase [Proteus terrae subsp. cibarius]
MFKKLFFQTKALPELSSQLDADIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLASYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWVRKSNQSTHLFLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADILFSSNVPSSVMLFSKSEWEAKQQTQAEPQSRSSEQPGL
PEASSSIELSNSAESPSTKSLEQDDELRHSIDVNNLQSAENDPRDGCEKPNNSYDGAISNNVSQQNEAAF
NLPESLAWLPEASSALIMAGEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGV
QEQGLLLKVSISKGLIALIDLSKQDAESVEPIQNEEASQRPSRTETANAQAKEPATRAERKQKPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAER
MFKKLFFQTKALPELSSQLDADIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLASYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWVRKSNQSTHLFLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADILFSSNVPSSVMLFSKSEWEAKQQTQAEPQSRSSEQPGL
PEASSSIELSNSAESPSTKSLEQDDELRHSIDVNNLQSAENDPRDGCEKPNNSYDGAISNNVSQQNEAAF
NLPESLAWLPEASSALIMAGEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGV
QEQGLLLKVSISKGLIALIDLSKQDAESVEPIQNEEASQRPSRTETANAQAKEPATRAERKQKPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAER
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16923 | GenBank | UVX23108 |
| Name | t4cp2_-_ICEPmiChn-TQ12 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67130.95 Da Isoelectric Point: 6.5123
>UVX23108.1 IncF plasmid conjugative transfer protein TraD [Proteus terrae subsp. cibarius]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQMG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKDEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
QRFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDTETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQMG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKDEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
QRFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDTETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16923 | GenBank | UVX23108 |
| Name | t4cp2_-_ICEPmiChn-TQ12 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67130.95 Da Isoelectric Point: 6.5123
>UVX23108.1 IncF plasmid conjugative transfer protein TraD [Proteus terrae subsp. cibarius]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQMG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKDEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
QRFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDTETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQMG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKDEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
QRFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDTETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 93463..113228
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_87 | 89243..90433 | + | 1191 | UVX23105 | hypothetical protein | - |
| Locus_88 | 90452..91147 | + | 696 | UVX23106 | hypothetical protein | - |
| Locus_89 | 91264..93414 | + | 2151 | UVX23107 | Conjugative transfer protein TraI relaxase | - |
| Locus_90 | 93463..95283 | + | 1821 | UVX23108 | IncF plasmid conjugative transfer protein TraD | virb4 |
| Locus_91 | 95293..95853 | + | 561 | UVX23109 | Conjugative transfer protein 234 | - |
| Locus_92 | 95840..96475 | + | 636 | UVX23110 | Conjugative transfer protein s043 | tfc7 |
| Locus_93 | 96501..96779 | - | 279 | UVX23111 | Antitoxin HigA | - |
| Locus_94 | 96776..97099 | - | 324 | UVX23112 | Toxin HigB | - |
| Locus_95 | 97280..97561 | + | 282 | UVX23113 | IncF plasmid conjugative transfer pilus assembly protein TraL | traL |
| Locus_96 | 97558..98184 | + | 627 | UVX23114 | IncF plasmid conjugative transfer pilus assembly protein TraE | traE |
| Locus_97 | 98165..99064 | + | 900 | UVX23115 | hypothetical protein | traK |
| Locus_98 | 99067..100356 | + | 1290 | UVX23116 | IncF plasmid conjugative transfer pilus assembly protein TraB | traB |
| Locus_99 | 100431..101003 | + | 573 | UVX23117 | Conjugative transfer protein TraV | traV |
| Locus_100 | 101000..101386 | + | 387 | UVX23118 | Conjugative transfer protein TraA | - |
| Locus_101 | 101565..102398 | + | 834 | UVX23119 | Ynd | - |
| Locus_102 | 102391..103080 | + | 690 | UVX23120 | Ync | - |
| Locus_103 | 103088..103327 | + | 240 | UVX23121 | Ync | - |
| Locus_104 | 103459..104151 | + | 693 | UVX23122 | hypothetical protein | trbB |
| Locus_105 | 104151..106550 | + | 2400 | UVX23123 | IncF plasmid conjugative transfer pilus assembly protein TraC | virb4 |
| Locus_106 | 106543..106890 | + | 348 | UVX23124 | Conjugative transfer protein 345 | - |
| Locus_107 | 106874..107386 | + | 513 | UVX23125 | Conjugative signal peptidase TrhF | - |
| Locus_108 | 107397..108521 | + | 1125 | UVX23126 | hypothetical protein | traW |
| Locus_109 | 108553..109533 | + | 981 | UVX23127 | IncF plasmid conjugative transfer pilus assembly protein TraU | traU |
| Locus_110 | 109536..113228 | + | 3693 | UVX23128 | IncF plasmid conjugative transfer protein TraN | traN |
| Locus_111 | 113721..114539 | + | 819 | UVX23129 | Aminoglycoside 3''-nucleotidyltransferase ANT(3'')-Ia (AadA family) | - |
| Locus_112 | 114636..115331 | + | 696 | UVX23130 | hypothetical protein | - |
| Locus_113 | 115544..116146 | - | 603 | UVX23131 | hypothetical protein | - |
| Locus_114 | 116514..116840 | + | 327 | UVX23132 | hypothetical protein | - |
| Locus_115 | 116856..117275 | + | 420 | UVX23133 | Single-stranded DNA-binding protein | - |
| Locus_116 | 117355..118173 | + | 819 | UVX23134 | Phage recombination protein Bet | - |
Host bacterium
| ID | 338 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEPmiChn-TQ12 | GenBank | ON390822 |
| Element size | 147546 bp | Coordinate of oriT [Strand] | 29801..30099 [+] |
| Host bacterium | Proteus terrae subsp. cibarius strain TQ12 ICEPmiChn-TQ12 mobile element | Coordinate of element | 1..147546 |
Cargo genes
| Drug resistance gene | tet(X6), floR, aph(6)-Id, aph(3'')-Ib, sul2, aph(3')-Ia, tet(A), aadA2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |