Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200379 |
| Name | oriT_ICEPmiChnJZ57  |
| Organism | Proteus mirabilis strain JZ57 ICEPmiChn_JZ57 mobile element |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | ON553412 (5388..5686 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 234..242, 254..262 (AAAGCCAAA..TTTGGCTTT) 45..50, 52..57 (CAAACG..CGTTTG) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEPmiChnJZ57
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14405 | GenBank | WBR55309 |
| Name | TraI_2_-_ICEPmiChnJZ57 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79760.84 Da Isoelectric Point: 5.2184
>WBR55309.1 hypothetical protein [Proteus mirabilis]
MFKNLFFQAKALPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTSNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDDKPIWLAMLHIVEADLLFSSNVPSSVRLFSKSEWEATPQTQAEPQSRSSEQPGL
PDASSSIEHGNSSESPSTKSSDQDDELRLASDVNNPQANENAPGDGCENPNNSYDGAISNNVNQHDAEAF
NLPESLAWLPEASSALVMVDEQIMIRYPDAVRHWCAPRKLLAELSRLDWLELDPANPTRKARTVTTKDGV
QEQGLLLKVSISKGLTALIDLPKQDAEPAAAIQNEEALQRPSRTKTTNAQAKEPATRAERKQKPIGPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPYVDHSADGIRVTIQTLRQVAKEHGIPAGQLLRGISASDEC
QFDEGETVLFTARAER
MFKNLFFQAKALPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTSNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDDKPIWLAMLHIVEADLLFSSNVPSSVRLFSKSEWEATPQTQAEPQSRSSEQPGL
PDASSSIEHGNSSESPSTKSSDQDDELRLASDVNNPQANENAPGDGCENPNNSYDGAISNNVNQHDAEAF
NLPESLAWLPEASSALVMVDEQIMIRYPDAVRHWCAPRKLLAELSRLDWLELDPANPTRKARTVTTKDGV
QEQGLLLKVSISKGLTALIDLPKQDAEPAAAIQNEEALQRPSRTKTTNAQAKEPATRAERKQKPIGPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPYVDHSADGIRVTIQTLRQVAKEHGIPAGQLLRGISASDEC
QFDEGETVLFTARAER
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16919 | GenBank | WBR55310 |
| Name | t4cp2_-_ICEPmiChnJZ57 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67102.94 Da Isoelectric Point: 6.2511
>WBR55310.1 IncF plasmid conjugative transfer protein TraD [Proteus mirabilis]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYLLYKLQMG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKTNTLAKQAMAMLLFYYERIQPIAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVDNRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYLLYKLQMG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKTNTLAKQAMAMLLFYYERIQPIAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVDNRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16919 | GenBank | WBR55310 |
| Name | t4cp2_-_ICEPmiChnJZ57 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67102.94 Da Isoelectric Point: 6.2511
>WBR55310.1 IncF plasmid conjugative transfer protein TraD [Proteus mirabilis]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYLLYKLQMG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKTNTLAKQAMAMLLFYYERIQPIAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVDNRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYLLYKLQMG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKTNTLAKQAMAMLLFYYERIQPIAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVDNRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 35041..59862
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_30 | 31504..32685 | + | 1182 | WBR55308 | Putative inner membrane protein | - |
| Locus_31 | 32842..34992 | + | 2151 | WBR55309 | hypothetical protein | - |
| Locus_32 | 35041..36861 | + | 1821 | WBR55310 | IncF plasmid conjugative transfer protein TraD | virb4 |
| Locus_33 | 36871..37431 | + | 561 | WBR55311 | Conjugative transfer protein 234 | - |
| Locus_34 | 37418..38053 | + | 636 | WBR55312 | Conjugative transfer protein s043 | tfc7 |
| Locus_35 | 38125..38763 | + | 639 | WBR55313 | hypothetical protein | - |
| Locus_36 | 38756..39862 | + | 1107 | WBR55314 | Transcriptional regulator | - |
| Locus_37 | 39999..40280 | + | 282 | WBR55315 | IncF plasmid conjugative transfer pilus assembly protein TraL | traL |
| Locus_38 | 40277..40903 | + | 627 | WBR55316 | IncF plasmid conjugative transfer pilus assembly protein TraE | traE |
| Locus_39 | 40884..41783 | + | 900 | WBR55317 | hypothetical protein | traK |
| Locus_40 | 41786..43075 | + | 1290 | WBR55318 | IncF plasmid conjugative transfer pilus assembly protein TraB | traB |
| Locus_41 | 43150..43722 | + | 573 | WBR55319 | Conjugative transfer protein TraV | traV |
| Locus_42 | 43719..44105 | + | 387 | WBR55320 | Conjugative transfer protein TraA | - |
| Locus_43 | 44284..45117 | + | 834 | WBR55321 | Ynd | - |
| Locus_44 | 45110..46048 | + | 939 | WBR55322 | Ync | - |
| Locus_45 | 46180..46872 | + | 693 | WBR55323 | hypothetical protein | trbB |
| Locus_46 | 46872..49271 | + | 2400 | WBR55324 | IncF plasmid conjugative transfer pilus assembly protein TraC | virb4 |
| Locus_47 | 49264..49611 | + | 348 | WBR55325 | Conjugative transfer protein 345 | - |
| Locus_48 | 49595..50107 | + | 513 | WBR55326 | Conjugative signal peptidase TrhF | - |
| Locus_49 | 50118..51242 | + | 1125 | WBR55327 | hypothetical protein | traW |
| Locus_50 | 51274..52254 | + | 981 | WBR55328 | IncF plasmid conjugative transfer pilus assembly protein TraU | traU |
| Locus_51 | 52257..55949 | + | 3693 | WBR55329 | IncF plasmid conjugative transfer protein TraN | traN |
| Locus_52 | 56408..57826 | + | 1419 | WBR55330 | hypothetical protein | - |
| Locus_53 | 57823..59862 | + | 2040 | WBR55331 | Bipolar DNA helicase HerA | virb4 |
| Locus_54 | 60108..60221 | + | 114 | WBR55332 | hypothetical protein | - |
| Locus_55 | 60271..60984 | - | 714 | WBR55333 | Endonuclease I precursor | - |
| Locus_56 | 60987..61553 | - | 567 | WBR55334 | Il-IS-2, transposase | - |
| Locus_57 | 61585..61806 | + | 222 | WBR55335 | hypothetical protein | - |
| Locus_58 | 62011..62274 | - | 264 | WBR55336 | Il-IS-2, transposase | - |
| Locus_59 | 62296..62808 | - | 513 | WBR55337 | Lipoprotein signal peptidase | - |
| Locus_60 | 62812..63708 | - | 897 | WBR55338 | Cobalt/zinc/cadmium resistance protein CzcD | - |
| Locus_61 | 63804..64211 | + | 408 | WBR55339 | Cd(II)/Pb(II)-responsive transcriptional regulator | - |
| Locus_62 | 64220..64339 | - | 120 | WBR55340 | S-(hydroxymethyl)glutathione dehydrogenase | - |
| Locus_63 | 64314..64436 | + | 123 | WBR55341 | hypothetical protein | - |
| Locus_64 | 64448..64669 | - | 222 | WBR55342 | hypothetical protein | - |
Host bacterium
| ID | 336 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEPmiChnJZ57 | GenBank | ON553412 |
| Element size | 116292 bp | Coordinate of oriT [Strand] | 5388..5686 [+] |
| Host bacterium | Proteus mirabilis strain JZ57 ICEPmiChn_JZ57 mobile element | Coordinate of element | 1..116292 |
Cargo genes
| Drug resistance gene | aph(6)-Id, aph(3'')-Ib, sul2, tet(C), aac(6')-Ib-cr, blaOXA-1, catB3, ARR-3, qacE, sul1, cfr, dfrA32, ere(A), aadA2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |