Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200377 |
| Name | oriT_ICEPmiChnJZ26  |
| Organism | Proteus mirabilis strain JZ26 ICEPmiChn-JZ26 mobile element |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | ON630400 (4838..5136 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 234..242, 254..262 (AAAGCCAAA..TTTGGCTTT) 45..50, 52..57 (CAAACG..CGTTTG) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEPmiChnJZ26
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14403 | GenBank | WBK56402 |
| Name | TraI_2_-_ICEPmiChnJZ26 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79777.71 Da Isoelectric Point: 5.2085
>WBK56402.1 hypothetical protein [Proteus mirabilis]
MFKNLFFQTKALPELSSQLDEDIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNSSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
VWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWVRKSNQSTNLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDGKPIWLPMLHMSEADLLFSSNVPSGVTLFSKSEWEATQQTQAEPQSRSSEHPDL
PEASSSIEHSNSAESPSTKPSDQDDELRHASDVNHLQANENVPGDGCEKPNNSYDGAISNNVNQHDAEAL
NLPESLTWLTEASNALVIVGEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGV
QEQGLLLKVSISKGLTALIDISKHDTESAAAIQNEEALPRPSRTETTNAQAKKTATRTERKQKPIAANAN
SSTDPKHVQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAER
MFKNLFFQTKALPELSSQLDEDIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNSSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
VWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWVRKSNQSTNLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDGKPIWLPMLHMSEADLLFSSNVPSGVTLFSKSEWEATQQTQAEPQSRSSEHPDL
PEASSSIEHSNSAESPSTKPSDQDDELRHASDVNHLQANENVPGDGCEKPNNSYDGAISNNVNQHDAEAL
NLPESLTWLTEASNALVIVGEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGV
QEQGLLLKVSISKGLTALIDISKHDTESAAAIQNEEALPRPSRTETTNAQAKKTATRTERKQKPIAANAN
SSTDPKHVQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAER
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16916 | GenBank | WBK56403 |
| Name | t4cp2_-_ICEPmiChnJZ26 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67172.97 Da Isoelectric Point: 6.5130
>WBK56403.1 IncF plasmid conjugative transfer protein TraD [Proteus mirabilis]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNTQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLTKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNTQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLTKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16916 | GenBank | WBK56403 |
| Name | t4cp2_-_ICEPmiChnJZ26 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67172.97 Da Isoelectric Point: 6.5130
>WBK56403.1 IncF plasmid conjugative transfer protein TraD [Proteus mirabilis]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNTQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLTKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNTQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLTKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 52579..72442
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_36 | 50380..52530 | + | 2151 | WBK56402 | hypothetical protein | - |
| Locus_37 | 52579..54399 | + | 1821 | WBK56403 | IncF plasmid conjugative transfer protein TraD | virb4 |
| Locus_38 | 54409..54969 | + | 561 | WBK56404 | Conjugative transfer protein 234 | - |
| Locus_39 | 54956..55591 | + | 636 | WBK56405 | Conjugative transfer protein s043 | tfc7 |
| Locus_40 | 55618..56205 | - | 588 | WBK56406 | hypothetical protein | - |
| Locus_41 | 56493..56774 | + | 282 | WBK56407 | IncF plasmid conjugative transfer pilus assembly protein TraL | traL |
| Locus_42 | 56771..57397 | + | 627 | WBK56408 | IncF plasmid conjugative transfer pilus assembly protein TraE | traE |
| Locus_43 | 57378..58277 | + | 900 | WBK56409 | hypothetical protein | traK |
| Locus_44 | 58280..59569 | + | 1290 | WBK56410 | IncF plasmid conjugative transfer pilus assembly protein TraB | traB |
| Locus_45 | 59644..60216 | + | 573 | WBK56411 | Conjugative transfer protein TraV | traV |
| Locus_46 | 60213..60599 | + | 387 | WBK56412 | Conjugative transfer protein TraA | - |
| Locus_47 | 60777..61595 | + | 819 | WBK56413 | Ynd | - |
| Locus_48 | 61603..62541 | + | 939 | WBK56414 | Ync | - |
| Locus_49 | 62673..63365 | + | 693 | WBK56415 | hypothetical protein | trbB |
| Locus_50 | 63365..65764 | + | 2400 | WBK56416 | IncF plasmid conjugative transfer pilus assembly protein TraC | virb4 |
| Locus_51 | 65757..66104 | + | 348 | WBK56417 | Conjugative transfer protein 345 | - |
| Locus_52 | 66088..66600 | + | 513 | WBK56418 | Conjugative signal peptidase TrhF | - |
| Locus_53 | 66608..67735 | + | 1128 | WBK56419 | hypothetical protein | traW |
| Locus_54 | 67719..68747 | + | 1029 | WBK56420 | IncF plasmid conjugative transfer pilus assembly protein TraU | traU |
| Locus_55 | 68750..72442 | + | 3693 | WBK56421 | IncF plasmid conjugative transfer protein TraN | traN |
| Locus_56 | 72533..75199 | - | 2667 | WBK56422 | ATPase involved in DNA repair | - |
| Locus_57 | 75371..76054 | - | 684 | WBK56423 | Endonuclease I precursor | - |
| Locus_58 | 76163..76765 | - | 603 | WBK56424 | hypothetical protein | - |
Host bacterium
| ID | 334 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEPmiChnJZ26 | GenBank | ON630400 |
| Element size | 106414 bp | Coordinate of oriT [Strand] | 4838..5136 [+] |
| Host bacterium | Proteus mirabilis strain JZ26 ICEPmiChn-JZ26 mobile element | Coordinate of element | 1..106414 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIA |