Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200375 |
| Name | oriT_ICEPmiChn15C1  |
| Organism | Proteus mirabilis strain Pm15C1 integrating |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | KX268685 (5939..6237 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 234..242, 254..262 (AAAGCCAAA..TTTGGCTTT) 45..50, 52..57 (CAAACG..CGTTTG) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEPmiChn15C1
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14401 | GenBank | AQM75190 |
| Name | TraI_2_-_ICEPmiChn15C1 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79658.88 Da Isoelectric Point: 5.6311
>AQM75190.1 Conjugative transfer protein TraI, relaxase [Proteus mirabilis]
MFKNLFFQTKVLPELSSQLDADIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNLRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTSNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWVRKSNQSTHLYLVWKSATKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVMIKDDKPIWLPMLHISEADLLFSSNLPSSVRLFSKSEWEATQQTQAEPQSRSSEHSDL
PEASTSIELSNSAESPSSKSSEQDDGLSRASDVNNPQATENAPGDGCEKPNNSYDGAISNNVNQHDAEAL
NLPESLAWLPEASSALVMVGEQILIHYPDAVRPWCAPRKLLAELSQLDWLELDPANPTRKARTITTKDGV
QEQGLLLKVSISKGLTALIDLPKQDAESAAAIQNKEASQRPSRTKTTNAQAKEPAKRAERKQKPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVAKEHCIPAGQLLRGISASDQC
QFDEGETVLFTAHAKR
MFKNLFFQTKVLPELSSQLDADIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNLRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTSNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWVRKSNQSTHLYLVWKSATKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVMIKDDKPIWLPMLHISEADLLFSSNLPSSVRLFSKSEWEATQQTQAEPQSRSSEHSDL
PEASTSIELSNSAESPSSKSSEQDDGLSRASDVNNPQATENAPGDGCEKPNNSYDGAISNNVNQHDAEAL
NLPESLAWLPEASSALVMVGEQILIHYPDAVRPWCAPRKLLAELSQLDWLELDPANPTRKARTITTKDGV
QEQGLLLKVSISKGLTALIDLPKQDAESAAAIQNKEASQRPSRTKTTNAQAKEPAKRAERKQKPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVAKEHCIPAGQLLRGISASDQC
QFDEGETVLFTAHAKR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16913 | GenBank | AQM75191 |
| Name | t4cp2_-_ICEPmiChn15C1 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67126.95 Da Isoelectric Point: 6.5130
>AQM75191.1 IncF plasmid conjugative transfer protein TraD [Proteus mirabilis]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPIAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPIAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 37899..59793
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_27 | 35700..37850 | + | 2151 | AQM75190 | Conjugative transfer protein TraI, relaxase | - |
| Locus_28 | 37899..39719 | + | 1821 | AQM75191 | IncF plasmid conjugative transfer protein TraD | virb4 |
| Locus_29 | 39729..40289 | + | 561 | AQM75192 | Conjugative transfer protein 234 | - |
| Locus_30 | 40276..40911 | + | 636 | AQM75193 | Conjugative transfer protein s043 | tfc7 |
| Locus_31 | 40938..41525 | - | 588 | AQM75194 | hypothetical protein | - |
| Locus_32 | 41814..42095 | + | 282 | AQM75195 | IncF plasmid conjugative transfer pilus assembly protein TraL | traL |
| Locus_33 | 42092..42718 | + | 627 | AQM75196 | IncF plasmid conjugative transfer pilus assembly protein TraE | traE |
| Locus_34 | 42702..43601 | + | 900 | AQM75197 | IncF plasmid conjugative transfer pilus assembly protein TraK | traK |
| Locus_35 | 43601..44890 | + | 1290 | AQM75198 | IncF plasmid conjugative transfer pilus assembly protein TraB | traB |
| Locus_36 | 44887..45537 | + | 651 | AQM75199 | Conjugative transfer protein TraV | traV |
| Locus_37 | 45534..45920 | + | 387 | AQM75200 | Conjugative transfer protein TraA | - |
| Locus_38 | 46098..46931 | + | 834 | AQM75201 | Ynd | - |
| Locus_39 | 46924..47862 | + | 939 | AQM75202 | Ync | - |
| Locus_40 | 48019..48174 | + | 156 | AQM75203 | hypothetical protein | - |
| Locus_41 | 48171..48344 | + | 174 | AQM75204 | hypothetical protein | - |
| Locus_42 | 48375..49295 | + | 921 | AQM75205 | Mobile element protein | - |
| Locus_43 | 49477..49902 | - | 426 | AQM75206 | hypothetical protein | - |
| Locus_44 | 50007..50480 | - | 474 | AQM75207 | Dihydrofolate reductase | - |
| Locus_45 | 50768..51727 | + | 960 | AQM75208 | Mobile element protein | - |
| Locus_46 | 51698..51838 | - | 141 | AQM75209 | hypothetical protein | - |
| Locus_47 | 51989..52933 | + | 945 | AQM75210 | IncF plasmid conjugative transfer pilus assembly protein TraF | traF |
| Locus_48 | 52936..54324 | + | 1389 | AQM75211 | IncF plasmid conjugative transfer pilus assembly protein TraH | traH |
| Locus_49 | 54328..57897 | + | 3570 | AQM75212 | IncF plasmid conjugative transfer protein TraG | traG |
| Locus_50 | 57930..58385 | - | 456 | AQM75213 | EexS | - |
| Locus_51 | 58419..58952 | - | 534 | AQM75214 | Transcriptional activator | - |
| Locus_52 | 58949..59248 | - | 300 | AQM75215 | Transcriptional activator | - |
| Locus_53 | 59245..59793 | - | 549 | AQM75216 | Soluble lytic murein transglycosylase | virB1 |
| Locus_54 | 59780..60442 | - | 663 | AQM75217 | hypothetical protein | - |
| Locus_55 | 60429..61298 | - | 870 | AQM75218 | hypothetical protein | - |
| Locus_56 | 61354..61605 | - | 252 | AQM75219 | hypothetical protein | - |
| Locus_57 | 61723..62370 | + | 648 | AQM75220 | Putative cI prophage repressor protein | - |
| Locus_58 | 62627..64216 | + | 1590 | AQM75221 | peptide chain release factor 3 | - |
Host bacterium
| ID | 332 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEPmiChn15C1 | GenBank | KX268685 |
| Element size | 64682 bp | Coordinate of oriT [Strand] | 5939..6237 [+] |
| Host bacterium | Proteus mirabilis strain Pm15C1 integrating | Coordinate of element | 1..64682 |
Cargo genes
| Drug resistance gene | dfrA1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |