Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200374 |
| Name | oriT_ICEPmiBra618  |
| Organism | Proteus mirabilis strain PmBR618 NODE_1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | WEKI01000001 (5496..5794 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 45..50, 52..57 (CAAACG..CGTTTG) 34..39, 51..56 (AAACGA..TCGTTT) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEPmiBra618
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAAACGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCGGCCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAAACGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCGGCCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14400 | GenBank | KAB7722857 |
| Name | TraI_2_GBN11_00650_ICEPmiBra618 |
UniProt ID | _ |
| Length | 684 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 684 a.a. Molecular weight: 76368.75 Da Isoelectric Point: 5.2604
>KAB7722857.1 phosphohydrolase [Proteus mirabilis]
MPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPCIDHLAAYVHLLPASEHHHHSGAGGLLRHSLEV
AFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGLFHDIGKPVSDLSITDEDGRYQWNPFLETLSQW
TTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELLAWLTQPGPEILQAMLEAIGNTDPEHVLSKLVI
EADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRLLASSQWLVNQRDARVWVRKSNQSTHLYLVWKS
AAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSASNERYESLAPEVLIKDGNPIWLPMLHISEADL
LFSSNVPSSVRLFGKSEWEATQQTQAEPQSRSSEHSDLPEASSSIEHSNSAQSPSTKPSDQDDELRHASD
VNHLQENENVPGDGCEKPNNSYDGAISNNVNQHDAEALNLPESLVWLQEASSALIMVGEQLLIRYPDAVR
PWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGVQEQGLLLKVSISKRLIALIDLSNQDAESVSVI
QNDEASQRLSRTETTNAQAKEPAKRAERKQKPIAPNTNSCTDPKHAQRQQMVNFVKDLPILLTDGDYPDV
DHSTDGIRVTIQTLRQVANEHGIPAGQLLRGISASDQCQFDEGETVLFTAHAKR
MPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPCIDHLAAYVHLLPASEHHHHSGAGGLLRHSLEV
AFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGLFHDIGKPVSDLSITDEDGRYQWNPFLETLSQW
TTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELLAWLTQPGPEILQAMLEAIGNTDPEHVLSKLVI
EADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRLLASSQWLVNQRDARVWVRKSNQSTHLYLVWKS
AAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSASNERYESLAPEVLIKDGNPIWLPMLHISEADL
LFSSNVPSSVRLFGKSEWEATQQTQAEPQSRSSEHSDLPEASSSIEHSNSAQSPSTKPSDQDDELRHASD
VNHLQENENVPGDGCEKPNNSYDGAISNNVNQHDAEALNLPESLVWLQEASSALIMVGEQLLIRYPDAVR
PWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGVQEQGLLLKVSISKRLIALIDLSNQDAESVSVI
QNDEASQRLSRTETTNAQAKEPAKRAERKQKPIAPNTNSCTDPKHAQRQQMVNFVKDLPILLTDGDYPDV
DHSTDGIRVTIQTLRQVANEHGIPAGQLLRGISASDQCQFDEGETVLFTAHAKR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16911 | GenBank | KAB7722683 |
| Name | traD_GBN11_00655_ICEPmiBra618 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67053.00 Da Isoelectric Point: 6.6444
>KAB7722683.1 conjugative transfer system coupling protein TraD [Proteus mirabilis]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLLKG
LKGKPLAFMALSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGELVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAV
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLLKG
LKGKPLAFMALSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGELVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16912 | GenBank | KAB7722696 |
| Name | traC_GBN11_00725_ICEPmiBra618 |
UniProt ID | _ |
| Length | 799 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 799 a.a. Molecular weight: 90112.69 Da Isoelectric Point: 6.1306
>KAB7722696.1 type IV secretion system protein TraC [Proteus mirabilis]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAAISAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTGEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGNGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAAISAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTGEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGNGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 146541..166222
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GBN11_00640 | 142552..143961 | - | 1410 | KAB7722681 | hypothetical protein | - |
| GBN11_00645 | 143962..144174 | - | 213 | KAB7722682 | helix-turn-helix domain-containing protein | - |
| GBN11_00650 | 144439..146493 | + | 2055 | KAB7722857 | phosphohydrolase | - |
| GBN11_00655 | 146541..148361 | + | 1821 | KAB7722683 | conjugative transfer system coupling protein TraD | virb4 |
| GBN11_00660 | 148371..148931 | + | 561 | KAB7722684 | conjugative transfer protein | - |
| GBN11_00665 | 148918..149553 | + | 636 | KAB7722685 | DUF4400 domain-containing protein | tfc7 |
| GBN11_00670 | 149580..150167 | - | 588 | KAB7722686 | plasmid-related protein | - |
| GBN11_00675 | 150181..150420 | - | 240 | KAB7722687 | hypothetical protein | - |
| GBN11_00680 | 150456..150737 | + | 282 | KAB7722688 | type IV conjugative transfer system protein TraL | traL |
| GBN11_00685 | 150734..151360 | + | 627 | KAB7722689 | pilus assembly protein | traE |
| GBN11_00690 | 151344..152240 | + | 897 | KAB7722858 | hypothetical protein | traK |
| GBN11_00695 | 152243..153532 | + | 1290 | KAB7722690 | conjugal transfer protein TraB | traB |
| GBN11_00700 | 153607..154179 | + | 573 | KAB7722691 | type IV conjugative transfer system lipoprotein TraV | traV |
| GBN11_00705 | 154176..154562 | + | 387 | KAB7722692 | hypothetical protein | - |
| GBN11_00710 | 154628..155548 | - | 921 | KAB7722693 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | - |
| GBN11_00715 | 155538..156074 | - | 537 | KAB7722694 | hypothetical protein | - |
| GBN11_00720 | 156453..157145 | + | 693 | KAB7722695 | thioredoxin fold domain-containing protein | trbB |
| GBN11_00725 | 157145..159544 | + | 2400 | KAB7722696 | type IV secretion system protein TraC | virb4 |
| GBN11_00730 | 159537..159884 | + | 348 | KAB7722697 | plasmid-related protein | - |
| GBN11_00735 | 159961..160380 | + | 420 | KAB7722859 | S26 family signal peptidase | - |
| GBN11_00740 | 160391..161515 | + | 1125 | KAB7722860 | pilus assembly protein | traW |
| GBN11_00745 | 161499..162527 | + | 1029 | KAB7722698 | conjugal transfer protein TraU | traU |
| GBN11_00750 | 162530..166222 | + | 3693 | KAB7722699 | conjugal transfer protein TraN | traN |
| GBN11_00755 | 166386..168200 | - | 1815 | KAB7722700 | DUF4365 domain-containing protein | - |
| GBN11_00760 | 168319..169002 | - | 684 | KAB7722701 | deoxyribonuclease I | - |
| GBN11_00765 | 169111..169713 | - | 603 | KAB7722702 | hypothetical protein | - |
| GBN11_00770 | 169928..171066 | + | 1139 | KAB7722703 | IS3 family transposase | - |
Host bacterium
| ID | 331 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEPmiBra618 | GenBank | WEKI01000001 |
| Element size | 351354 bp | Coordinate of oriT [Strand] | 5496..5794 [+] |
| Host bacterium | Proteus mirabilis strain PmBR618 NODE_1 | Coordinate of element | 130187..195100 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |