Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200373 |
| Name | oriT_ICEPmiBra614  |
| Organism | Proteus mirabilis strain PmBR614 NODE_4 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | WEKJ01000004 (3569..3867 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 45..50, 52..57 (CAAACG..CGTTTG) 34..39, 51..56 (AAACGA..TCGTTT) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEPmiBra614
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAAACGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCGGCCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAAACGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCGGCCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14399 | GenBank | KAB7723770 |
| Name | TraI_2_GBN13_03965_ICEPmiBra614 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79552.57 Da Isoelectric Point: 5.2136
>KAB7723770.1 phosphohydrolase [Proteus mirabilis]
MFKNLFFQTKALPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNLRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTSNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERALATKSA
SNERYESLAPEVLIKDDKPIWLGMLHIVEADLLFSSNVPSGVTLFSKPEWEATQQTQAEPQSRSCEQPGL
SEASSSIEHSSSAESPSTKSSEQDDELRHASDVSNPQATENASGDECEKPNNSYDGAISNNVNQHDTEAL
NLPESLAWLPEASSALVMVGEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGV
QEQGLLLKVSISKGLTALIDLSKQDEEPAAAIQNEEASQRTSRTKTTNAQAKEPATRAERKQKPIVANAN
SSTDPKHAQRQQMVSFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANAHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAKR
MFKNLFFQTKALPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNLRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTSNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERALATKSA
SNERYESLAPEVLIKDDKPIWLGMLHIVEADLLFSSNVPSGVTLFSKPEWEATQQTQAEPQSRSCEQPGL
SEASSSIEHSSSAESPSTKSSEQDDELRHASDVSNPQATENASGDECEKPNNSYDGAISNNVNQHDTEAL
NLPESLAWLPEASSALVMVGEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGV
QEQGLLLKVSISKGLTALIDLSKQDEEPAAAIQNEEASQRTSRTKTTNAQAKEPATRAERKQKPIVANAN
SSTDPKHAQRQQMVSFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANAHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAKR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16909 | GenBank | KAB7723771 |
| Name | traD_GBN13_03970_ICEPmiBra614 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67139.99 Da Isoelectric Point: 6.7828
>KAB7723771.1 conjugative transfer system coupling protein TraD [Proteus mirabilis]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMALSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FSARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDRPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMALSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FSARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDRPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16910 | GenBank | KAB7723784 |
| Name | traC_GBN13_04040_ICEPmiBra614 |
UniProt ID | _ |
| Length | 799 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 799 a.a. Molecular weight: 90170.73 Da Isoelectric Point: 5.9735
>KAB7723784.1 type IV secretion system protein TraC [Proteus mirabilis]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVNEMTDRNWLAALSAQLNWGKDASWRNPSPVRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVNEMTDRNWLAALSAQLNWGKDASWRNPSPVRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 87997..107862
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GBN13_03965 | 85798..87948 | + | 2151 | KAB7723770 | phosphohydrolase | - |
| GBN13_03970 | 87997..89817 | + | 1821 | KAB7723771 | conjugative transfer system coupling protein TraD | virb4 |
| GBN13_03975 | 89827..90387 | + | 561 | KAB7723772 | conjugative transfer protein | - |
| GBN13_03980 | 90374..91009 | + | 636 | KAB7723773 | DUF4400 domain-containing protein | tfc7 |
| GBN13_03985 | 91036..91623 | - | 588 | KAB7723774 | plasmid-related protein | - |
| GBN13_03990 | 91637..91876 | - | 240 | KAB7723775 | hypothetical protein | - |
| GBN13_03995 | 91912..92193 | + | 282 | KAB7723776 | type IV conjugative transfer system protein TraL | traL |
| GBN13_04000 | 92190..92816 | + | 627 | KAB7723777 | pilus assembly protein | traE |
| GBN13_04005 | 92800..93696 | + | 897 | KAB7723883 | hypothetical protein | traK |
| GBN13_04010 | 93699..94988 | + | 1290 | KAB7723778 | conjugal transfer protein TraB | traB |
| GBN13_04015 | 95063..95635 | + | 573 | KAB7723779 | type IV conjugative transfer system lipoprotein TraV | traV |
| GBN13_04020 | 95632..96018 | + | 387 | KAB7723780 | hypothetical protein | - |
| GBN13_04025 | 96239..97015 | + | 777 | KAB7723781 | hypothetical protein | - |
| GBN13_04030 | 97023..97961 | + | 939 | KAB7723782 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | - |
| GBN13_04035 | 98093..98785 | + | 693 | KAB7723783 | thioredoxin fold domain-containing protein | trbB |
| GBN13_04040 | 98785..101184 | + | 2400 | KAB7723784 | type IV secretion system protein TraC | virb4 |
| GBN13_04045 | 101177..101524 | + | 348 | KAB7723785 | plasmid-related protein | - |
| GBN13_04050 | 101601..102020 | + | 420 | KAB7723786 | S26 family signal peptidase | - |
| GBN13_04055 | 102031..103155 | + | 1125 | KAB7723884 | pilus assembly protein | traW |
| GBN13_04060 | 103139..104167 | + | 1029 | KAB7723787 | conjugal transfer protein TraU | traU |
| GBN13_04065 | 104170..107862 | + | 3693 | KAB7723788 | conjugal transfer protein TraN | traN |
| GBN13_04070 | 108026..109840 | - | 1815 | KAB7723789 | DUF4365 domain-containing protein | - |
| GBN13_04075 | 109959..110642 | - | 684 | KAB7723790 | deoxyribonuclease I | - |
| GBN13_04080 | 110751..111353 | - | 603 | KAB7723791 | hypothetical protein | - |
| GBN13_04085 | 111721..112047 | + | 327 | KAB7723792 | plasmid-related protein | - |
| GBN13_04090 | 112063..112482 | + | 420 | KAB7723793 | single-stranded DNA-binding protein | - |
Host bacterium
| ID | 330 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEPmiBra614 | GenBank | WEKJ01000004 |
| Element size | 215088 bp | Coordinate of oriT [Strand] | 3569..3867 [+] |
| Host bacterium | Proteus mirabilis strain PmBR614 NODE_4 | Coordinate of element | 54801..139479 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |