Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200368 |
| Name | oriT_ICEPgs6Chn1  |
| Organism | Proteus genomosp. 6 strain T60 sequence |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | MN507533 (5974..6272 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 234..242, 254..262 (AAAGCCAAA..TTTGGCTTT) 45..50, 52..57 (CAAACG..CGTTTG) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEPgs6Chn1
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14394 | GenBank | QIM09847 |
| Name | traI_-_ICEPgs6Chn1 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79644.48 Da Isoelectric Point: 5.3431
>QIM09847.1 Conjugative transfer protein TraI, relaxase [Proteus genomosp. 6]
MFKNLFFQAKAIPELSSQLDADIPRYPPFLKGLPAASPKDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSSVRLFSKSEWEATQQTQAESQSRSSEHSDL
PEASSSIEHGNSSESPSTNSSEQDDELSHASDVNHLQATENAPGDGCEKPNNSYDGAISNNVNQHDAEAL
NLTESLVWLPEASSALVMVGEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGV
HEQGLLLKVSISKGLTALIDFSKQDAESVTAIQNEEASQRPSRTETANAQAKELATRAERKQKPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANAHGIPAGQLLRGISASDEC
QFDEGETVLFTAHAKR
MFKNLFFQAKAIPELSSQLDADIPRYPPFLKGLPAASPKDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSSVRLFSKSEWEATQQTQAESQSRSSEHSDL
PEASSSIEHGNSSESPSTNSSEQDDELSHASDVNHLQATENAPGDGCEKPNNSYDGAISNNVNQHDAEAL
NLTESLVWLPEASSALVMVGEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGV
HEQGLLLKVSISKGLTALIDFSKQDAESVTAIQNEEASQRPSRTETANAQAKELATRAERKQKPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANAHGIPAGQLLRGISASDEC
QFDEGETVLFTAHAKR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16901 | GenBank | QIM09848 |
| Name | traD_-_ICEPgs6Chn1 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67059.86 Da Isoelectric Point: 6.5728
>QIM09848.1 IncF plasmid conjugative transfer protein TraD [Proteus genomosp. 6]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLHKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPSFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLHKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPSFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16902 | GenBank | QIM09862 |
| Name | traC_-_ICEPgs6Chn1 |
UniProt ID | _ |
| Length | 799 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 799 a.a. Molecular weight: 90171.75 Da Isoelectric Point: 5.9735
>QIM09862.1 IncF plasmid conjugative transfer pilus assembly protein TraC [Proteus genomosp. 6]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 65686..85551
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_57 | 61466..62656 | + | 1191 | QIM09845 | hypothetical protein | - |
| Locus_58 | 62657..63370 | + | 714 | QIM09846 | hypothetical protein | - |
| Locus_59 | 63488..65638 | + | 2151 | QIM09847 | Conjugative transfer protein TraI, relaxase | - |
| Locus_60 | 65686..67506 | + | 1821 | QIM09848 | IncF plasmid conjugative transfer protein TraD | virb4 |
| Locus_61 | 67516..68076 | + | 561 | QIM09849 | Conjugative transfer protein 234 | - |
| Locus_62 | 68063..68698 | + | 636 | QIM09850 | Conjugative transfer protein | tfc7 |
| Locus_63 | 68725..69312 | - | 588 | QIM09851 | hypothetical protein | - |
| Locus_64 | 69326..69565 | - | 240 | QIM09852 | hypothetical protein | - |
| Locus_65 | 69601..69882 | + | 282 | QIM09853 | IncF plasmid conjugative transfer pilus assembly protein TraL | traL |
| Locus_66 | 69879..70505 | + | 627 | QIM09854 | IncF plasmid conjugative transfer pilus assembly protein TraE | traE |
| Locus_67 | 70486..71385 | + | 900 | QIM09855 | IncF plasmid conjugative transfer pilus assembly protein TraK | traK |
| Locus_68 | 71388..72677 | + | 1290 | QIM09856 | IncF plasmid conjugative transfer pilus assembly protein TraB | traB |
| Locus_69 | 72674..73324 | + | 651 | QIM09857 | Conjugative transfer protein TraV | traV |
| Locus_70 | 73321..73707 | + | 387 | QIM09858 | Conjugative transfer protein TraA | - |
| Locus_71 | 73886..74719 | + | 834 | QIM09859 | Ynd | - |
| Locus_72 | 74712..75650 | + | 939 | QIM09860 | Ync | - |
| Locus_73 | 75782..76474 | + | 693 | QIM09861 | Thiol:disulfide involved in conjugative transfer | trbB |
| Locus_74 | 76474..78873 | + | 2400 | QIM09862 | IncF plasmid conjugative transfer pilus assembly protein TraC | virb4 |
| Locus_75 | 78866..79213 | + | 348 | QIM09863 | Conjugative transfer protein 345 | - |
| Locus_76 | 79197..79709 | + | 513 | QIM09864 | Conjugative signal peptidase TrhF | - |
| Locus_77 | 79720..80844 | + | 1125 | QIM09865 | IncF plasmid conjugative transfer pilus assembly protein TraW | traW |
| Locus_78 | 80876..81856 | + | 981 | QIM09866 | IncF plasmid conjugative transfer pilus assembly protein TraU | traU |
| Locus_79 | 81859..85551 | + | 3693 | QIM09867 | IncF plasmid conjugative transfer protein TraN | traN |
| Locus_80 | 85619..85777 | - | 159 | QIM09868 | hypothetical protein | - |
| Locus_81 | 86234..88063 | + | 1830 | QIM09869 | putative exonuclease | - |
| Locus_82 | 88072..89976 | + | 1905 | QIM09870 | ATP-dependent DNA helicase | - |
Host bacterium
| ID | 325 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEPgs6Chn1 | GenBank | MN507533 |
| Element size | 116235 bp | Coordinate of oriT [Strand] | 5974..6272 [+] |
| Host bacterium | Proteus genomosp. 6 strain T60 sequence | Coordinate of element | 1..116235 |
Cargo genes
| Drug resistance gene | floR, aph(6)-Id, aph(3'')-Ib, aph(3')-Ia, aac(3)-IVa, aph(4)-Ia, tet(X6), sul2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |