Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200362 |
| Name | oriT_PAGI-2  |
| Organism | Pseudomonas aeruginosa strain C |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | AF440523 (96341..96658 [+], 318 nt) |
| oriT length | 318 nt |
| IRs (inverted repeats) | 238..243, 251..256 (CCAGCC..GGCTGG) 199..206, 215..222 (TAAAAGGC..GCCTTTTA) 197..202, 207..212 (TTTAAA..TTTAAA) |
| Location of nic site | 44..45 |
| Conserved sequence flanking the nic site |
TCGTGCCTGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 318 nt
>oriT_PAGI-2
CCATGGCCGATCGATCGTGCTTGCGCCAGCGAAGAAAGGTCGTGCCTGCGCCAGTGGTCTGCTGGGCCAGCTCGACGGGAAGCAGGTGGAAGGGATGCCCGCACGCCTGTGGCAGCACCTGTCGCTGCGCCAGGGCAATCAACTCGTCGCGCATGGCGAAGCACTGGCTGGCCCAGGCCTCGAAGTCCCCTTTACCTTTAAAAGGCTTTAAAAGGCCTTTTAGAGAGGCCGCGTGTTCCAGCCGCATGAAGGCTGGCTGTTCCAGACGACGGAAGTAGCGCGGTTCGCGGTTCGGGTCGCTCATGCCGGTTCGTCCTC
CCATGGCCGATCGATCGTGCTTGCGCCAGCGAAGAAAGGTCGTGCCTGCGCCAGTGGTCTGCTGGGCCAGCTCGACGGGAAGCAGGTGGAAGGGATGCCCGCACGCCTGTGGCAGCACCTGTCGCTGCGCCAGGGCAATCAACTCGTCGCGCATGGCGAAGCACTGGCTGGCCCAGGCCTCGAAGTCCCCTTTACCTTTAAAAGGCTTTAAAAGGCCTTTTAGAGAGGCCGCGTGTTCCAGCCGCATGAAGGCTGGCTGTTCCAGACGACGGAAGTAGCGCGGTTCGCGGTTCGGGTCGCTCATGCCGGTTCGTCCTC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14390 | GenBank | AAN62129 |
| Name | ORF C36_-_PAGI-2 |
UniProt ID | _ |
| Length | 630 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 630 a.a. Molecular weight: 68882.39 Da Isoelectric Point: 6.8467
>AAN62129.1 conserved hypothetical protein [Pseudomonas aeruginosa]
MLSLFQRKRPAVATAPTPPPASDLPKGLMRPESAASLLATPRRQKLLEHIWQRTSLSRKQFAVLYRAPLE
RYAELVQAFPASESHHHAYPGGMLDHGLEIVAYALKLRQSHLLPIGASPEDQAAQAEAWTAAVAYAALLH
DIGKIAVDLHVELADGSLWHPWYGPLHQPYRFRYRDDREYRLHSAATGLLYRQLLDTQLLDWLSDYRDLW
GPLLYVLAGQYEHAGVLGELVVQADRASVAQELGGDPARAMAAPKHALQRKLLDGLRYLLKEELKLNQPE
ASDGWLTEDALWLVSKTVSDKLRAHLLSQGIDGIPANNTAVFNVLQDHGMLQPTLDGKAVWRATVTSNAG
WTHSFTLLRLAPALIWEAGERPAPFAGTVAIDTSPVEKHAAAASSPEVAAKPTPGGQESPPWEGGSAADP
IIAPLAEAMPDVMEDLLTMVGMGDSSATQQDESTIPTELSATRPEAPPPSIVASSPSSPAPAATPAATTV
QPSGEHFMAWLKQGIASRRLIINDAKALVHTVSDTAYLVSPGVFQRYAQEHPQLAAIARQEKLEPWQWAQ
KRFEKLAAHRKQASGLNIWTCHVSGPRKSRQLHGYLLTQPGALFERVPPNNPYLCLFNEEAGREVSLAAK
MLSLFQRKRPAVATAPTPPPASDLPKGLMRPESAASLLATPRRQKLLEHIWQRTSLSRKQFAVLYRAPLE
RYAELVQAFPASESHHHAYPGGMLDHGLEIVAYALKLRQSHLLPIGASPEDQAAQAEAWTAAVAYAALLH
DIGKIAVDLHVELADGSLWHPWYGPLHQPYRFRYRDDREYRLHSAATGLLYRQLLDTQLLDWLSDYRDLW
GPLLYVLAGQYEHAGVLGELVVQADRASVAQELGGDPARAMAAPKHALQRKLLDGLRYLLKEELKLNQPE
ASDGWLTEDALWLVSKTVSDKLRAHLLSQGIDGIPANNTAVFNVLQDHGMLQPTLDGKAVWRATVTSNAG
WTHSFTLLRLAPALIWEAGERPAPFAGTVAIDTSPVEKHAAAASSPEVAAKPTPGGQESPPWEGGSAADP
IIAPLAEAMPDVMEDLLTMVGMGDSSATQQDESTIPTELSATRPEAPPPSIVASSPSSPAPAATPAATTV
QPSGEHFMAWLKQGIASRRLIINDAKALVHTVSDTAYLVSPGVFQRYAQEHPQLAAIARQEKLEPWQWAQ
KRFEKLAAHRKQASGLNIWTCHVSGPRKSRQLHGYLLTQPGALFERVPPNNPYLCLFNEEAGREVSLAAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16891 | GenBank | AAN62159 |
| Name | ORF C65_-_PAGI-2 |
UniProt ID | _ |
| Length | 729 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 729 a.a. Molecular weight: 80777.58 Da Isoelectric Point: 7.3460
>AAN62159.1 conserved hypothetical protein [Pseudomonas aeruginosa]
MSGKQPVEVLLRPAVELYTVAACAGAAFLCLVAPWSLALSPAMGIGSALAFGAYGAIRYRDARIILRYRR
NIRRLPRYVMTSKDVPVSQQRLFVGRGFLWEQKHTHRLMQTYRPEFRRYVEPTPAYRLTRRLEERLEFAP
LPLSRLPKLTGWDAPFNPVRPLPPVGGLPRLHGIEPDEVDVSLPLGERVGHSLVLGTTRVGKTRLAELFV
TQDIRRRNAAGEHEVVIVIDPKGDADLLKRMYVEAQRAGREGEFYIFHLGWPEISARYNAVGRFGRISEV
ATRIAGQLSGEGNSAAFREFAWRFVNIIARALVELGQRPDYMLIQRHVINIDALFIEYAQHYFAKTEPKA
WEVIVQIEAKLNEKNIPRNMIGREKRVVALEQYLSQARNYDPVLDGLRSAVRYDKTYFDKIVASLLPLLE
KLTSGKIAQLLAPNYSDLADPRPIFDWMQVIRKRAVVYVGLDALSDAEVAAAVGNSMFSDLVSVAGHIYK
HGIDDGLPGASAGARVPINVHADEFNELMGDEFIPLINKGGGAGLQVTAYTQTLSDIEARIGNRAKAGQV
IGNFNNLFMLRVRETATAELLTRQLPKVEVYTTTIVSGATDSSDIRGATDFTSNTQDRISMASVPMIEPS
HVVGLPKGQCFALLQGGQLWKVRMPLPAPDPDEVMPTDLQQLAGYMRQSYSEATQWWEFTSSAALPHAAL
PDDLLDDAAPAEPDAVATGADDSTGEAAP
MSGKQPVEVLLRPAVELYTVAACAGAAFLCLVAPWSLALSPAMGIGSALAFGAYGAIRYRDARIILRYRR
NIRRLPRYVMTSKDVPVSQQRLFVGRGFLWEQKHTHRLMQTYRPEFRRYVEPTPAYRLTRRLEERLEFAP
LPLSRLPKLTGWDAPFNPVRPLPPVGGLPRLHGIEPDEVDVSLPLGERVGHSLVLGTTRVGKTRLAELFV
TQDIRRRNAAGEHEVVIVIDPKGDADLLKRMYVEAQRAGREGEFYIFHLGWPEISARYNAVGRFGRISEV
ATRIAGQLSGEGNSAAFREFAWRFVNIIARALVELGQRPDYMLIQRHVINIDALFIEYAQHYFAKTEPKA
WEVIVQIEAKLNEKNIPRNMIGREKRVVALEQYLSQARNYDPVLDGLRSAVRYDKTYFDKIVASLLPLLE
KLTSGKIAQLLAPNYSDLADPRPIFDWMQVIRKRAVVYVGLDALSDAEVAAAVGNSMFSDLVSVAGHIYK
HGIDDGLPGASAGARVPINVHADEFNELMGDEFIPLINKGGGAGLQVTAYTQTLSDIEARIGNRAKAGQV
IGNFNNLFMLRVRETATAELLTRQLPKVEVYTTTIVSGATDSSDIRGATDFTSNTQDRISMASVPMIEPS
HVVGLPKGQCFALLQGGQLWKVRMPLPAPDPDEVMPTDLQQLAGYMRQSYSEATQWWEFTSSAALPHAAL
PDDLLDDAAPAEPDAVATGADDSTGEAAP
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 67412..94997
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_35 | 62441..63436 | + | 996 | AAN62128 | putative transcriptional regulator | - |
| Locus_36 | 63484..65376 | - | 1893 | AAN62129 | conserved hypothetical protein | - |
| Locus_37 | 65692..66318 | + | 627 | AAN62130 | conserved hypothetical protein | - |
| Locus_38 | 66331..66963 | + | 633 | AAN62131 | conserved hypothetical protein | - |
| Locus_39 | 67037..67396 | + | 360 | AAN62132 | conserved hypothetical protein | - |
| Locus_40 | 67412..68959 | - | 1548 | AAN62133 | hypothetical protein | tfc19 |
| Locus_41 | 68975..69331 | - | 357 | AAN62134 | hypothetical protein | tfc18 |
| Locus_42 | 69328..70722 | - | 1395 | AAN62135 | hypothetical protein | tfc22 |
| Locus_43 | 70732..71682 | - | 951 | AAN62136 | hypothetical protein | tfc23 |
| Locus_44 | 71679..72125 | - | 447 | AAN62137 | hypothetical protein | tfc24 |
| Locus_45 | 72290..72784 | - | 495 | AAN62140 | putative DNA repair protein RadC | - |
| Locus_46 | 72960..73724 | - | 765 | AAN62139 | conserved hypothetical protein | - |
| Locus_47 | 73749..76580 | - | 2832 | AAN62141 | hypothetical protein | virb4 |
| Locus_48 | 76641..77081 | - | 441 | AAN62142 | hypothetical protein | tfc15 |
| Locus_49 | 77062..78480 | - | 1419 | AAN62143 | hypothetical protein | tfc14 |
| Locus_50 | 78470..79381 | - | 912 | AAN62144 | hypothetical protein | tfc13 |
| Locus_51 | 79378..80070 | - | 693 | AAN62145 | hypothetical protein | tfc12 |
| Locus_52 | 80067..80465 | - | 399 | AAN62146 | hypothetical protein | tfc11 |
| Locus_53 | 80478..80837 | - | 360 | AAN62147 | hypothetical protein | tfc10 |
| Locus_54 | 80854..81087 | - | 234 | AAN62148 | hypothetical protein | tfc9 |
| Locus_55 | 81084..81467 | - | 384 | AAN62149 | hypothetical protein | tfc8 |
| Locus_56 | 81671..82141 | + | 471 | AAN62150 | putative excisionase | - |
| Locus_57 | 82138..82713 | + | 576 | AAN62151 | conserved hypothetical protein | - |
| Locus_58 | 82731..83645 | + | 915 | AAN62152 | conserved hypothetical protein | - |
| Locus_59 | 83642..84112 | + | 471 | AAN62153 | hypothetical protein | - |
| Locus_60 | 84109..84609 | + | 501 | AAN62154 | hypothetical protein | - |
| Locus_61 | 84609..85511 | + | 903 | AAN62155 | hypothetical protein | - |
| Locus_62 | 85316..86275 | + | 960 | AAN62156 | hypothetical protein | - |
| Locus_63 | 86285..88909 | + | 2625 | AAN62157 | hypothetical protein | - |
| Locus_64 | 88950..89699 | - | 750 | AAN62158 | hypothetical protein | tfc7 |
| Locus_65 | 89696..91885 | - | 2190 | AAN62159 | conserved hypothetical protein | - |
| Locus_66 | 91890..92438 | - | 549 | AAN62160 | hypothetical protein | tfc5 |
| Locus_67 | 92435..93025 | - | 591 | AAN62161 | conserved hypothetical protein | virB1 |
| Locus_68 | 93007..93744 | - | 738 | AAN62162 | hypothetical protein | tfc3 |
| Locus_69 | 93757..94401 | - | 645 | AAN62163 | hypothetical protein | - |
| Locus_70 | 94398..94997 | - | 600 | AAN62164 | conserved hypothetical protein | tfc2 |
| Locus_71 | 95136..97415 | - | 2280 | AAN62165 | conserved hypothetical plasmid protein | - |
| Locus_72 | 97552..97857 | - | 306 | AAN62166 | hypothetical protein | - |
| Locus_73 | 97947..98267 | - | 321 | AAN62167 | hypothetical protein | - |
| Locus_74 | 98318..99427 | - | 1110 | AAN62168 | conserved hypothetical plasmid protein | - |
Host bacterium
| ID | 319 | Element type | ICE (Integrative and conjugative element) |
| Element name | PAGI-2 | GenBank | AF440523 |
| Element size | 158230 bp | Coordinate of oriT [Strand] | 96341..96658 [+] |
| Host bacterium | Pseudomonas aeruginosa strain C | Coordinate of element | 27286..132240 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | merA, merT, merR2, pbrA |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA3 |