Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200348
Name   oriT2_ICEAsp1 in_silico
Organism   Actinobacillus sp. ICEAsp1 mobile element
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   MW030510 ( 62093..62130 [-], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      1..6, 10..15  (CTTTAC..GTAAAG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 38 nt

>oriT2_ICEAsp1
CTTTACGAAGTAAAGTATAGTGGGTTATACTTTACATG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14373 GenBank   QRX38479
Name   TraI_2_-_ICEAsp1 insolico UniProt ID   _
Length   641 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 641 a.a.        Molecular weight: 72772.01 Da        Isoelectric Point: 6.5126

>QRX38479.1 TraI [Actinobacillus sp.]
MIKILRTLIKKTKSVATNTSMEQLSIGWYEPLSANKLLSSPLRQQYLKTIWQNVSMEPQMFNVLYKTPIE
KYAEIVQLLPASESHHHSHIGGMLDHGLEVISIATKLRQSYILPQNSAPEEQAKQRDVWTAVVIYAALLH
DIGKIAVDIEIILQNGARWFPWQGKPAQPYNFRYIKNRDYSLHSILGSFFANNLIPKEAFDWVAPFPQAF
SSLMYFISGHPDKSGVLSEIIQRADQISVTMALGGDPSKLSEKTKLSFAKQLHIALRNVVEKFKLNSPKG
GCDGWLTEDGLWLMSKSTADNIRSYLMLQGVSVPAQNGKIFDELQTHRLIQKTDNATAIWNGRVISNAGW
APPKPFTLLKVSPEIIWDNLDDRPEVFDGRIDIVNEKGEVTTATALKFEPTETLETETSELVVQNEEQEQ
FITTTKTVSNDLVLSGENFHKSNESMEIDYVLDMFSPVTERVLQDSNIYEPAFNETVNITQENSTQITTS
EQKYLGDVAQLTQDSPKIYNEHSIDAQKFIQWVKAGVLSGRLVINRPNAKLHIVEKHIFLVTPSIFQLYL
KEVANSTDKKSWELLQKQFQNLGVHKRQHTSDDSRNIWTCSIVGPNKQSFLNGYLIEDVKLFIGDKILLN
NQWLKLQGDLQ

  Protein domains


Predicted by InterproScan.

Putative conjugal transfer nickase/helicase TraI C-term (TraI_2_C)

(516-636)

Putative helicase (TraI_2)

(27-338)


  Protein structure



No available structure.




T4CP

ID   16868 GenBank   QRX38412
Name   t4cp2_-_ICEAsp1 insolico UniProt ID   _
Length   723 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 723 a.a.        Molecular weight: 80902.65 Da        Isoelectric Point: 8.2080

>QRX38412.1 TraD [Actinobacillus sp.]
MSNKFLVESLLRPPVEFYTSAVCGASAALCAIAPWTVALTPSIGYGLSAGFTVLGIKRALEGWKVVKYHR
NLKRLPRYAMSSKEIPVMKKHLFLGLGFRWTQKHTQRLHDCFQANGEKYLAPSKCYTFVKEFEKKHEESF
ITKLTSKDSPFNPFRPLPPVEGIPAIHGIELNEVNVTIPLASRVGHMLVLGTTRVGKTRLAELLVTQDIH
RGKTFNEREVVIFFDPKGDADILKRMYAEAKRAGRENEFYVFHLGWPDISARYNAVGRFNKITEVASRIS
GQLSGAGNSAAFKEFAWRFVNIIARALVELGRRPDYEQIAKFVRSIDSLFLDYAKFYFDQRDKNIWLAIA
EVAANIDQKNLSLAMRGRSEMVVAVDQYIRDNKIFDPVLDGLCSAVRYDKTYFDKIVASLLPLLEKLTSG
KVAELLSPDYSNISDTRPIFDWEEIIRKRGIVYIGLDALSDLTVASAVGNSMFADLVSMAGHIYKFGINE
GLPQSLTHKNSKIAINVHCDEFNELIGDEFIPLINKGGGAGVQVTAYTQTISDIKARTGDASKAGQIVGN
FNSLVMLRVKEQATAEVLTKNLHEVTVLEVMALSSTTDSSDPGSDKHFGSNTGDRVTQKQKPMLSAADIT
NLPKGQAFALIEGSQLWKIRMPLPVEDKDDLMPKDLQQLAKYMQDNYQTTETWWEGVNSYQPNSDAVAAF
ENLSKTLFPEEKNNENTFRSIDN

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(507-634)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 14954..34907

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Locus_11 11592..11750 + 159 QRX38402 hypothetical protein -
Locus_12 12275..12712 + 438 QRX38403 hypothetical protein -
Locus_13 12761..13087 + 327 QRX38404 hypothetical protein -
Locus_14 13186..13515 + 330 QRX38405 hypothetical protein -
Locus_15 13595..14242 + 648 QRX38406 hypothetical protein -
Locus_16 14317..14802 + 486 QRX38407 RadC -
Locus_17 14954..15622 + 669 QRX38408 PilL tfc2
Locus_18 15716..16468 + 753 QRX38409 integrating conjugative element protein tfc3
Locus_19 16447..17172 + 726 QRX38410 hypothetical protein -
Locus_20 17169..17678 + 510 QRX38411 integrating conjugative element protein tfc5
Locus_21 17675..19846 + 2172 QRX38412 TraD -
Locus_22 20153..20848 + 696 QRX38413 integrating conjugative element membrane protein tfc7
Locus_23 21009..21332 + 324 QRX38414 conjugal transfer protein tfc8
Locus_24 21329..21562 + 234 QRX38415 integrating conjugative element protein tfc9
Locus_25 21592..21966 + 375 QRX38416 integrating conjugative element membrane protein tfc10
Locus_26 21977..22351 + 375 QRX38417 conjugative transfer region protein tfc11
Locus_27 22348..22986 + 639 QRX38418 integrating conjugative element protein tfc12
Locus_28 23000..23848 + 849 QRX38419 integrating conjugative element protein tfc13
Locus_29 23858..25297 + 1440 QRX38420 integrating conjugative element protein tfc14
Locus_30 25308..25715 + 408 QRX38421 conjugal transfer lipoprotein tfc15
Locus_31 25734..28553 + 2820 QRX38422 TrbE virb4
Locus_32 28550..28960 + 411 QRX38423 hypothetical protein tfc17
Locus_33 28987..29334 + 348 QRX38424 hypothetical protein tfc18
Locus_34 29339..30814 + 1476 QRX38425 TraG tfc19
Locus_35 30851..31030 - 180 QRX38426 hypothetical protein -
Locus_36 31057..31506 - 450 QRX38427 hypothetical protein -
Locus_37 31528..33495 - 1968 QRX38428 PilT tfc22
Locus_38 33504..34475 - 972 QRX38429 TraU tfc23
Locus_39 34485..34907 - 423 QRX38430 integrating conjugative element protein tfc24
Locus_40 35302..35640 + 339 QRX38431 hypothetical protein -
Locus_41 35713..35853 + 141 QRX38432 hypothetical protein -
Locus_42 36087..37058 + 972 QRX38433 TraC -
Locus_43 37069..37299 + 231 QRX38434 hypothetical protein -
Locus_44 37421..38152 + 732 QRX38435 hypothetical protein -
Locus_45 38294..38635 - 342 QRX38436 hypothetical protein -
Locus_46 38715..39368 - 654 QRX38437 resolvase, N terminal domain protein -

Host bacterium

ID   305 Element type   ICE (Integrative and conjugative element)
Element name   ICEAsp1 GenBank   MW030510
Element size   72978 bp Coordinate of oriT [Strand]   60932..60972 [-]; 62093..62130 [-]
Host bacterium   Actinobacillus sp. ICEAsp1 mobile element Coordinate of element   1..72978

Cargo genes

Drug resistance gene   tet(B), blaROB-1, aac(6')-aph(2''), aph(3')-Ia, aph(6)-Id, catT, sul2, erm(T), mcr-1.1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -