Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200346 |
| Name | oriT_ICEAplChn1  |
| Organism | Actinobacillus pleuropneumoniae sequence |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | KX196444 (6177..6475 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 45..50, 52..57 (CAAACG..CGTTTG) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEAplChn1
GCTCTGTTTGGCGGCGGATAACCTAGCCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATTTTCATGGCAATACGTCTTTTTAGGCGTTTTTAAGTGAAATCGGCCTGCATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGTAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCGGATAACCTAGCCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATTTTCATGGCAATACGTCTTTTTAGGCGTTTTTAAGTGAAATCGGCCTGCATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGTAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14370 | GenBank | ART88951 |
| Name | traI_-_ICEAplChn1 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79705.82 Da Isoelectric Point: 5.1572
>ART88951.1 TraI [Actinobacillus pleuropneumoniae]
MFKNLFFQTKALPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQREFQRLIQPC
IDNLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWVRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLAIKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSGVTLFSKPEWEATQQTRAEPQIRSSEQPGL
PEASSSIELSNSAESPSSKSSDQDDELRHASDVNHLQANENAPGDECEKPNNSYDGAISNNVNQHDAEAL
NLPESLAWLPEASSALVMVDEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTKDGV
QEQGLLLKVSISKGLTALIDLSKQDAEAAVAIQNEEASQRPSRTETANAQAKEPATRAKRKQKPIAANAN
SSTDPKHAQRQQMVNFVKDLPILLTDGNYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAER
MFKNLFFQTKALPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQREFQRLIQPC
IDNLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWVRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLAIKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSGVTLFSKPEWEATQQTRAEPQIRSSEQPGL
PEASSSIELSNSAESPSSKSSDQDDELRHASDVNHLQANENAPGDECEKPNNSYDGAISNNVNQHDAEAL
NLPESLAWLPEASSALVMVDEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTKDGV
QEQGLLLKVSISKGLTALIDLSKQDAEAAVAIQNEEASQRPSRTETANAQAKEPATRAKRKQKPIAANAN
SSTDPKHAQRQQMVNFVKDLPILLTDGNYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAER
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16863 | GenBank | ART88952 |
| Name | traD_-_ICEAplChn1 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67071.93 Da Isoelectric Point: 6.5125
>ART88952.1 TraD [Actinobacillus pleuropneumoniae]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQMG
LKGKPLAFMALSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGIENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQMG
LKGKPLAFMALSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGIENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16864 | GenBank | ART88966 |
| Name | traC_-_ICEAplChn1 |
UniProt ID | _ |
| Length | 799 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 799 a.a. Molecular weight: 90216.80 Da Isoelectric Point: 6.1321
>ART88966.1 TraC [Actinobacillus pleuropneumoniae]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMSDRNWLAALSAQLNWGKDASWRNPSPIRREADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSSTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMSDRNWLAALSAQLNWGKDASWRNPSPIRREADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSSTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 49713..70818
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_40 | 45493..46683 | + | 1191 | ART88949 | hypothetical protein | - |
| Locus_41 | 46684..47397 | + | 714 | ART88950 | hypothetical protein | - |
| Locus_42 | 47514..49664 | + | 2151 | ART88951 | TraI | - |
| Locus_43 | 49713..51533 | + | 1821 | ART88952 | TraD | virb4 |
| Locus_44 | 51543..52103 | + | 561 | ART88953 | putative conjugative transfer protein | - |
| Locus_45 | 52090..52725 | + | 636 | ART88954 | putative conjugation coupling factor | tfc7 |
| Locus_46 | 52752..53135 | - | 384 | ART89001 | hypothetical protein | - |
| Locus_47 | 53296..54318 | - | 1023 | ART88955 | Tnp transposase | - |
| Locus_48 | 54370..54579 | - | 210 | ART88956 | hypothetical protein | - |
| Locus_49 | 54868..55149 | + | 282 | ART88957 | TraL | traL |
| Locus_50 | 55146..55772 | + | 627 | ART88958 | TraE | traE |
| Locus_51 | 55756..56652 | + | 897 | ART88959 | TraK | traK |
| Locus_52 | 56655..57944 | + | 1290 | ART88960 | TraB | traB |
| Locus_53 | 58019..58591 | + | 573 | ART88961 | TraV | traV |
| Locus_54 | 58588..58974 | + | 387 | ART88962 | TraA | - |
| Locus_55 | 59153..59986 | + | 834 | ART88963 | MosA | - |
| Locus_56 | 59976..60917 | + | 942 | ART88964 | MosT | - |
| Locus_57 | 61049..61741 | + | 693 | ART88965 | DsbC | trbB |
| Locus_58 | 61741..64140 | + | 2400 | ART88966 | TraC | virb4 |
| Locus_59 | 64133..64480 | + | 348 | ART88967 | putative conjugative transfer protein | - |
| Locus_60 | 64464..64976 | + | 513 | ART88968 | TrhF | - |
| Locus_61 | 64987..66111 | + | 1125 | ART88969 | TraW | traW |
| Locus_62 | 66095..67123 | + | 1029 | ART88970 | TraU | traU |
| Locus_63 | 67126..70818 | + | 3693 | ART88971 | TraN | traN |
| Locus_64 | 70982..72796 | - | 1815 | ART88972 | hypothetical protein | - |
| Locus_65 | 72915..73598 | - | 684 | ART88973 | putative endonuclease I precursor | - |
| Locus_66 | 73707..74309 | - | 603 | ART88974 | hypothetical protein | - |
| Locus_67 | 74677..75003 | + | 327 | ART89003 | hypothetical protein | - |
| Locus_68 | 75019..75438 | + | 420 | ART88975 | Ssb | - |
Host bacterium
| ID | 304 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEAplChn1 | GenBank | KX196444 |
| Element size | 100848 bp | Coordinate of oriT [Strand] | 6177..6475 [+] |
| Host bacterium | Actinobacillus pleuropneumoniae sequence | Coordinate of element | 1..100848 |
Cargo genes
| Drug resistance gene | tet(59), erm(42), floR, aph(6)-Id, aph(3')-VIa, aph(3'')-Ib, sul2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |