Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200344 |
| Name | oriT_ICEAmaD7  |
| Organism | Alteromonas macleodii strain D7 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP014323 (2890..3188 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 45..50, 52..57 (CAAACG..CGTTTG) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEAmaD7
GCGCTGTTTGGCGGCGGATGACTGAGCCAAAAAAATCGATACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATTTTCATGGCAATACGTCTTTTTAGGCGTTTTTAAGTGAAATCGGCCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGCGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCGCTGTTTGGCGGCGGATGACTGAGCCAAAAAAATCGATACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATTTTCATGGCAATACGTCTTTTTAGGCGTTTTTAAGTGAAATCGGCCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGCGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14368 | GenBank | AMJ97716 |
| Name | TraI_2_AVL55_05785_ICEAmaD7 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79697.61 Da Isoelectric Point: 5.0021
>AMJ97716.1 phosphohydrolase [Alteromonas macleodii]
MFKKLFFQTKALPELSSQLDADIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQREFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTSNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTDLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDGNPIWLPMLHISEADLLFSSNVPSSVRLFSKSEWESTQQTQAEPQSSSSEQPGL
PEASSSIELSNSDESPSSKSSDQDDELRHASDVNHLEENENVPGDGCEKPNNSYDGAISNNVNQYDADSL
NLPESLAWLPEASSALIMVGEQALIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTKDGV
QEQGLLLKVSISKGLTALIDISKQDEESVAAIQNKEASQRPSRTETTSAQAKELATRAERKQTPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVAKEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAKR
MFKKLFFQTKALPELSSQLDADIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQREFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTSNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTDLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDGNPIWLPMLHISEADLLFSSNVPSSVRLFSKSEWESTQQTQAEPQSSSSEQPGL
PEASSSIELSNSDESPSSKSSDQDDELRHASDVNHLEENENVPGDGCEKPNNSYDGAISNNVNQYDADSL
NLPESLAWLPEASSALIMVGEQALIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTKDGV
QEQGLLLKVSISKGLTALIDISKQDEESVAAIQNKEASQRPSRTETTSAQAKELATRAERKQTPIAPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVAKEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAKR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16859 | GenBank | AMJ97717 |
| Name | t4cp2_AVL55_05790_ICEAmaD7 |
UniProt ID | _ |
| Length | 607 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 607 a.a. Molecular weight: 67154.02 Da Isoelectric Point: 6.6444
>AMJ97717.1 conjugal transfer protein [Alteromonas macleodii]
MKENAYEMPWRTNYEAMAAAGWLVVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQK
GLKGKPLAFMALSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGV
EPKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGS
PERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPD
LKTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLE
GLLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLT
DAMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFA
DFAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGE
RLMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQK
GLKGKPLAFMALSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGV
EPKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGS
PERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPD
LKTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLE
GLLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLT
DAMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFA
DFAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGE
RLMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16860 | GenBank | AMJ97743 |
| Name | t4cp2_AVL55_05955_ICEAmaD7 |
UniProt ID | _ |
| Length | 799 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 799 a.a. Molecular weight: 90260.85 Da Isoelectric Point: 6.1321
>AMJ97743.1 conjugal transfer protein TraC [Alteromonas macleodii]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQSIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAAISAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKRFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQSIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAAISAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKRFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1300452..1311221
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AVL55_05775 | 1296232..1297422 | + | 1191 | AMJ97714 | hypothetical protein | - |
| AVL55_05780 | 1297423..1298136 | + | 714 | AMJ97715 | hypothetical protein | - |
| AVL55_05785 | 1298253..1300403 | + | 2151 | AMJ97716 | phosphohydrolase | - |
| AVL55_05790 | 1300452..1302275 | + | 1824 | AMJ97717 | conjugal transfer protein | virb4 |
| AVL55_05795 | 1302285..1302845 | + | 561 | AMJ97718 | conjugative transfer protein | - |
| AVL55_05800 | 1302832..1303467 | + | 636 | AMJ97719 | conjugal transfer protein | tfc7 |
| AVL55_05805 | 1303494..1304081 | - | 588 | AMJ97720 | plasmid-related protein | - |
| AVL55_05810 | 1304700..1305122 | + | 423 | AMJ97721 | hypothetical protein | - |
| AVL55_05815 | 1305176..1305502 | - | 327 | AMJ97722 | hypothetical protein | - |
| AVL55_05820 | 1305545..1305868 | + | 324 | AMJ97723 | hypothetical protein | - |
| AVL55_05825 | 1305962..1307125 | - | 1164 | AMK00349 | porin | - |
| AVL55_05830 | 1307250..1307462 | - | 213 | AMJ97724 | hypothetical protein | - |
| AVL55_05835 | 1307498..1307779 | + | 282 | AMJ97725 | conjugal transfer protein TraL | traL |
| AVL55_05840 | 1307776..1308402 | + | 627 | AMJ97726 | pilus assembly protein | traE |
| AVL55_05845 | 1308386..1309282 | + | 897 | AMK00350 | hypothetical protein | traK |
| AVL55_05850 | 1309285..1310574 | + | 1290 | AMJ97727 | conjugal transfer protein TraB | traB |
| AVL55_05855 | 1310571..1311221 | + | 651 | AMJ97728 | conjugal transfer protein TraV | traV |
| AVL55_05860 | 1311218..1311607 | + | 390 | AMJ97729 | hypothetical protein | - |
| AVL55_05865 | 1312016..1314511 | + | 2496 | AMJ97730 | ATPase | - |
| AVL55_05870 | 1314537..1315001 | + | 465 | AMJ97731 | Cu(I)-responsive transcriptional regulator | - |
| AVL55_05875 | 1315088..1315537 | - | 450 | Protein_1151 | MerR family transcriptional regulator | - |
| AVL55_05880 | 1315684..1315902 | + | 219 | AMJ97732 | hypothetical protein | - |
Host bacterium
| ID | 302 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEAmaD7 | GenBank | CP014323 |
| Element size | 4575623 bp | Coordinate of oriT [Strand] | 2890..3188 [+] |
| Host bacterium | Alteromonas macleodii strain D7 | Coordinate of element | 1261745..1375908 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | golT |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIA |