Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200343 |
| Name | oriT_ICE-2  |
| Organism | Burkholderia multivorans ATCC BAA-247 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP009832 (67857..68351 [-], 495 nt) |
| oriT length | 495 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 495 nt
>oriT_ICE-2
GACGCGGGGAATTGCTGGACCAACTCGGCGTAGCGTTCCAGCGGGGCGAGGTACAGCGTGGCGAACTGCCGGCGCGAGAGCGAGGTGCGCTGCCAGATGTGTTCCAGCAGCTTCTGCCGGCGGGGTGTCGCCAGCAGCGATGCGGCCGGCTCCGGCCGCATCAGCCCTTTGCCGGGCTCGGCGGAAGGTTTGGGCGTCGGCGCACTGGCGGCGGGCGGCACCCGTTTGCGTTGGAACAGCGAGAGCATGGCGAACCCCGGACGGCGGGCCGGTCGGGAGAGCCTTTTGGCCTTTTTGGGTAGGGCCTTTCCCCTTGGCCCCATTCCCTTGCCCCTTCCCCAGCCCTTTGGCCTTTACGGAAACCTTTGGATATAGGGCAACGACGCCTGCCAGACCACAACCAATGCGGGTTTGGCCCAAGTCGGATTGATACGGGAAGGCTGGCTGTGCCGCTCCTACGATGGAGCCGTTTCATCGACCGGACGGAGCACCATG
GACGCGGGGAATTGCTGGACCAACTCGGCGTAGCGTTCCAGCGGGGCGAGGTACAGCGTGGCGAACTGCCGGCGCGAGAGCGAGGTGCGCTGCCAGATGTGTTCCAGCAGCTTCTGCCGGCGGGGTGTCGCCAGCAGCGATGCGGCCGGCTCCGGCCGCATCAGCCCTTTGCCGGGCTCGGCGGAAGGTTTGGGCGTCGGCGCACTGGCGGCGGGCGGCACCCGTTTGCGTTGGAACAGCGAGAGCATGGCGAACCCCGGACGGCGGGCCGGTCGGGAGAGCCTTTTGGCCTTTTTGGGTAGGGCCTTTCCCCTTGGCCCCATTCCCTTGCCCCTTCCCCAGCCCTTTGGCCTTTACGGAAACCTTTGGATATAGGGCAACGACGCCTGCCAGACCACAACCAATGCGGGTTTGGCCCAAGTCGGATTGATACGGGAAGGCTGGCTGTGCCGCTCCTACGATGGAGCCGTTTCATCGACCGGACGGAGCACCATG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14367 | GenBank | AJY18156 |
| Name | traI_NP80_998_ICE-2 |
UniProt ID | _ |
| Length | 615 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 615 a.a. Molecular weight: 67179.36 Da Isoelectric Point: 7.2382
>AJY18156.1 integrating conjugative element relaxase, PFGI-1 class [Burkholderia multivorans ATCC BAA-247]
MLSLFQRKRVPPAASAPTPKPSAEPGKGLMRPEPAASLLATPRRQKLLEHIWQRTSLSRRQFATLYLAPL
ERYAELVQQFPASEAHHHAYPGGMLDHGLEIVAYALKLRQSHLLPAGATPEAQAAQAEAWTAGTAYAALL
HDIGKIAVDLHVEYADGTVWHPWHGPLRRPYRFRYRREREYRLHSAATGLLYARLLDPGIFDWLAGYPDL
WAALLYVLAGQYEHAGMLGELVVQADQASVAQELGGDPSKALAAPRHALQRKLLDGLRYLLKEEFKLNQP
QASDGWLTQDALWLVSKTVSDKLRAHLLSQGIDGIPASNTAVFNVLQDHGIALATPDGKAIWKATVTSDA
GWSHSFTFLKLSPAMVWDAADRPAPFAGRVQADEEQGALTPQAPAAEAPGLESADTVPPSTAPTAPVPAG
NGVAALLDLLSDTAPPVAPEPPSDPEPASPPSPTVLPQVSPAPTRDRPEPSGEHFMAWLRQGIQTHKLII
NDAKALVHSVAGTAYLVSPGVFQRYAQEHLQIAALAKPENLEGWQWVQKRFEKLSLHRKQASGLNIWTCE
VTGPRKSRRLHGYLLSAPEALFQEMPPDNPYLRLLTEAVKRENSAAGNKNDNEQG
MLSLFQRKRVPPAASAPTPKPSAEPGKGLMRPEPAASLLATPRRQKLLEHIWQRTSLSRRQFATLYLAPL
ERYAELVQQFPASEAHHHAYPGGMLDHGLEIVAYALKLRQSHLLPAGATPEAQAAQAEAWTAGTAYAALL
HDIGKIAVDLHVEYADGTVWHPWHGPLRRPYRFRYRREREYRLHSAATGLLYARLLDPGIFDWLAGYPDL
WAALLYVLAGQYEHAGMLGELVVQADQASVAQELGGDPSKALAAPRHALQRKLLDGLRYLLKEEFKLNQP
QASDGWLTQDALWLVSKTVSDKLRAHLLSQGIDGIPASNTAVFNVLQDHGIALATPDGKAIWKATVTSDA
GWSHSFTFLKLSPAMVWDAADRPAPFAGRVQADEEQGALTPQAPAAEAPGLESADTVPPSTAPTAPVPAG
NGVAALLDLLSDTAPPVAPEPPSDPEPASPPSPTVLPQVSPAPTRDRPEPSGEHFMAWLRQGIQTHKLII
NDAKALVHSVAGTAYLVSPGVFQRYAQEHLQIAALAKPENLEGWQWVQKRFEKLSLHRKQASGLNIWTCE
VTGPRKSRRLHGYLLSAPEALFQEMPPDNPYLRLLTEAVKRENSAAGNKNDNEQG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16858 | GenBank | AJY18924 |
| Name | traD_NP80_977_ICE-2 |
UniProt ID | _ |
| Length | 719 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 719 a.a. Molecular weight: 80086.07 Da Isoelectric Point: 8.2263
>AJY18924.1 conjugative coupling factor TraD, PFGI-1 class [Burkholderia multivorans ATCC BAA-247]
MAHSQAVEVLLRPAVELYTVAVCAGAALLCLVAPWSLALSPLLGLGSALAFLTFGAIRFRDAWAILRYRR
NIRRLPRYVMTSRDVPVSQQRLFVGRGFRWDQRHTHRLMQTYRPEFRRYVEPTAIYRAARYFEERLEFAP
YPLSKLAKALAWDSPLNPARPLPPVGGLPRLHGIEPHEVDVTLPLAERVGHSLVLGTTRVGKTRLAELFI
TQDIRRKVGGQHEVVIVFDPKGDTDLLKRMYVEAKRAGREGEFYVFHLGWPDISARYNAVGRFGRISEVA
TRIAGQLSGEGNSAAFREFAWRFVNIIARALVELGQRPDYLLIQRHVINIDALFIEYAQHYFARSEPKAW
EVIVQLEAKLNDKNIPRNMIGREKRVVALEQYLSQVRIYDPVLDGLRSAVRYDRTYFDKIVASLLPLLEK
LTTGKIAQLLAPNYSDLGDPRPIFDWMQIIRKRAVVYVGLDALSDAEVAAAVGNSMFSDLVSVAGHIYKF
GIDDGLPGASAGMKVPINVHADEFNELMGDEFIPMVNKGGGAGVQVTAYTQTMSDIEARIGSRAKAGQVI
GNFNNLFMLRVRETITAELLTRQLPQVEVYTTSLVSGATDSSDPSGNTAFTSNTQDRVTSTSVPLIEPAH
VVGLPKGQCFALIEGGHLWKVRMPLPAPDPDEAMPKDLQELAGYMRQHYVEAGDWWDGKGIPGLQDQALP
DDLLADFKQMASVDEGAAA
MAHSQAVEVLLRPAVELYTVAVCAGAALLCLVAPWSLALSPLLGLGSALAFLTFGAIRFRDAWAILRYRR
NIRRLPRYVMTSRDVPVSQQRLFVGRGFRWDQRHTHRLMQTYRPEFRRYVEPTAIYRAARYFEERLEFAP
YPLSKLAKALAWDSPLNPARPLPPVGGLPRLHGIEPHEVDVTLPLAERVGHSLVLGTTRVGKTRLAELFI
TQDIRRKVGGQHEVVIVFDPKGDTDLLKRMYVEAKRAGREGEFYVFHLGWPDISARYNAVGRFGRISEVA
TRIAGQLSGEGNSAAFREFAWRFVNIIARALVELGQRPDYLLIQRHVINIDALFIEYAQHYFARSEPKAW
EVIVQLEAKLNDKNIPRNMIGREKRVVALEQYLSQVRIYDPVLDGLRSAVRYDRTYFDKIVASLLPLLEK
LTTGKIAQLLAPNYSDLGDPRPIFDWMQIIRKRAVVYVGLDALSDAEVAAAVGNSMFSDLVSVAGHIYKF
GIDDGLPGASAGMKVPINVHADEFNELMGDEFIPMVNKGGGAGVQVTAYTQTMSDIEARIGSRAKAGQVI
GNFNNLFMLRVRETITAELLTRQLPQVEVYTTSLVSGATDSSDPSGNTAFTSNTQDRVTSTSVPLIEPAH
VVGLPKGQCFALIEGGHLWKVRMPLPAPDPDEAMPKDLQELAGYMRQHYVEAGDWWDGKGIPGLQDQALP
DDLLADFKQMASVDEGAAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1037258..1057520
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NP80_969 | 1032851..1033507 | + | 657 | AJY17745 | hypothetical protein | - |
| NP80_970 | 1033575..1034693 | + | 1119 | AJY19465 | methyltransferase domain protein | - |
| NP80_971 | 1034828..1037110 | + | 2283 | AJY19395 | hypothetical protein | - |
| NP80_972 | 1037258..1037854 | + | 597 | AJY19408 | putative pilL protein | tfc2 |
| NP80_973 | 1037851..1038495 | + | 645 | AJY19288 | hypothetical protein | - |
| NP80_974 | 1038505..1039227 | + | 723 | AJY18948 | hypothetical protein | tfc3 |
| NP80_975 | 1039212..1039796 | + | 585 | AJY18923 | transglycosylase SLT domain protein | virB1 |
| NP80_976 | 1039793..1040347 | + | 555 | AJY18244 | integrating conjugative element, family domain protein | tfc5 |
| NP80_977 | 1040376..1042535 | + | 2160 | AJY18924 | conjugative coupling factor TraD, PFGI-1 class | - |
| NP80_978 | 1042532..1043281 | + | 750 | AJY18309 | hypothetical protein | tfc7 |
| NP80_979 | 1043396..1043782 | + | 387 | AJY17814 | hypothetical protein | tfc8 |
| NP80_980 | 1043779..1044018 | + | 240 | AJY19736 | hypothetical protein | tfc9 |
| NP80_981 | 1044042..1044407 | + | 366 | AJY18394 | hypothetical protein | tfc10 |
| NP80_982 | 1044420..1044845 | + | 426 | AJY17205 | hypothetical protein | tfc11 |
| NP80_983 | 1044842..1045534 | + | 693 | AJY18318 | hypothetical protein | tfc12 |
| NP80_984 | 1045531..1046448 | + | 918 | AJY18227 | hypothetical protein | tfc13 |
| NP80_985 | 1046438..1047916 | + | 1479 | AJY18329 | hypothetical protein | tfc14 |
| NP80_986 | 1047897..1048331 | + | 435 | AJY19104 | putative signal recognition particle GTPase | tfc15 |
| NP80_987 | 1048331..1051225 | + | 2895 | AJY19185 | conjugative transfer ATPase, family | virb4 |
| NP80_988 | 1051239..1051985 | + | 747 | AJY19767 | DSBA-like thioredoxin domain protein | - |
| NP80_989 | 1052180..1052674 | + | 495 | AJY18654 | DNA repair RadC family protein | - |
| NP80_990 | 1052831..1053277 | + | 447 | AJY18303 | hypothetical protein | tfc24 |
| NP80_991 | 1053274..1054224 | + | 951 | AJY19268 | traU family protein | tfc23 |
| NP80_992 | 1054234..1055637 | + | 1404 | AJY17557 | integrating conjugative element protein, family | tfc22 |
| NP80_993 | 1055634..1055987 | + | 354 | AJY17769 | hypothetical protein | tfc18 |
| NP80_994 | 1056003..1057520 | + | 1518 | AJY18726 | hypothetical protein | tfc19 |
| NP80_995 | 1057526..1057897 | - | 372 | AJY17588 | hypothetical protein | - |
| NP80_996 | 1057984..1058448 | - | 465 | AJY19766 | toxin with endonuclease activity YhaV family protein | - |
| NP80_997 | 1058448..1058765 | - | 318 | AJY18328 | transcriptional regulator, AbrB family domain protein | - |
| NP80_998 | 1059079..1060926 | + | 1848 | AJY18156 | integrating conjugative element relaxase, PFGI-1 class | - |
| NP80_999 | 1060953..1061099 | - | 147 | AJY19187 | helix-turn-helix family protein | - |
| NP80_1000 | 1061363..1061989 | + | 627 | AJY19508 | lysR substrate binding domain protein | - |
Host bacterium
| ID | 301 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICE-2 | GenBank | CP009832 |
| Element size | 3428264 bp | Coordinate of oriT [Strand] | 67857..68351 [-] |
| Host bacterium | Burkholderia multivorans ATCC BAA-247 | Coordinate of element | 990976..1064196 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |