Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200308
• Name: oriT_ICE_SsaFDA259_Tn916
• Organism: Streptococcus salivarius strain FDAARGOS_259
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP020451 (2501..2633 [+], 133 nt)
• oriT length: 133 nt
• IRs (inverted repeats): 65..70, 83..88 (AAATCC..GGATTT) 6..12, 23..29 (ACCCCCC..GGGGGGT)
• Location of nic site: 75..76
• Conserved sequence flanking the nic site: TTTGGTTACA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 133 nt

>oriT_ICE_SsaFDA259_Tn916
ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_A6J87_08615_ICE_SsaFDA259_Tn916

• ID: 14349
• GenBank: ARC49410
• Name: Rep_trans_A6J87_08615_ICE_SsaFDA259_Tn916
• UniProt ID: _
• Length: 401 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 401 a.a.
• Molecular weight: 47398.10 Da
• Isoelectric Point: 6.5091

>ARC49410.1 XRE family transcriptional regulator [Streptococcus salivarius]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

Protein domains

Predicted by InterproScan.

• Helix-turn-helix (HTH_3) (15-53)

• Replication initiation factor (Rep_trans) (169-373)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (73-161)

Protein structure

No available structure.

Auxiliary protein

ARC49429_ICE_SsaFDA259_Tn916

• ID: 7874
• GenBank: ARC49429
• Name: ARC49429_ICE_SsaFDA259_Tn916
• UniProt ID: _
• Length: 405 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 405 a.a.
• Molecular weight: 47021.92 Da
• Isoelectric Point: 9.7978

>ARC49429.1 site-specific integrase [Streptococcus salivarius]
MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKLVATDRVPAGKRDCISLREKIAELQKD
IHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSE
NGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYS
KNYDEILILLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQ
AFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFC
TNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA

Protein domains

Predicted by InterproScan.

• Phage integrase family (Phage_integrase) (190-383)

• DNA binding domain of tn916 integrase (Integrase_DNA) (1-71)

Protein structure

No available structure.

T4CP

tcpA_A6J87_08605_ICE_SsaFDA259_Tn916

• ID: 16841
• GenBank: ARC49408
• Name: tcpA_A6J87_08605_ICE_SsaFDA259_Tn916
• UniProt ID: _
• Length: 461 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 461 a.a.
• Molecular weight: 53370.27 Da
• Isoelectric Point: 9.0687

>ARC49408.1 DNA translocase FtsK [Streptococcus salivarius]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (217-301)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1792339..1803749

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6J87_08585	1789383..1791359	+	1977	ARC49405	glucosyl transferase	-
A6J87_08590	1791690..1791838	+	149	Protein_1633	glucosyl transferase	-
A6J87_10820	1792197..1792316	+	120	AVH84131	hypothetical protein	-
A6J87_08595	1792339..1792653	+	315	ARC49406	DUF961 domain-containing protein	orf23
A6J87_08600	1792669..1793055	+	387	ARC49407	DUF961 domain-containing protein	orf23
A6J87_08605	1793084..1794469	+	1386	ARC49408	DNA translocase FtsK	virb4
A6J87_08610	1794472..1794624	+	153	ARC49409	conjugal transfer protein	-
A6J87_08615	1794647..1795852	+	1206	ARC49410	XRE family transcriptional regulator	-
A6J87_08620	1795895..1796116	+	222	ARC49411	hypothetical protein	orf19
A6J87_08625	1796233..1796730	+	498	ARC49412	antirestriction protein ArdA	-
A6J87_08630	1796705..1797211	+	507	ARC49413	conjugal transfer protein	orf17a
A6J87_08635	1797195..1799642	+	2448	ARC49414	ATP/GTP-binding protein	virb4
A6J87_08640	1799645..1801822	+	2178	ARC49415	hypothetical protein	orf15
A6J87_08645	1801819..1802820	+	1002	ARC49416	peptidase P60	orf14
A6J87_08650	1802817..1803749	+	933	ARC49417	conjugal transfer protein	orf13
A6J87_10825	1803793..1803855	+	63	Protein_1647	XRE family transcriptional regulator	-
A6J87_08655	1804024..1804110	+	87	ARC49418	tetracycline resistance protein	-
A6J87_08660	1804126..1806045	+	1920	ARC49419	tetracycline resistance ribosomal protection protein Tet(M)	-
A6J87_08665	1806146..1806331	+	186	ARC49420	conjugal transfer protein	-
A6J87_08670	1806391..1806528	-	138	Protein_1651	XRE family transcriptional regulator	-
A6J87_08675	1806812..1806923	+	112	Protein_1652	peptide-binding protein	-
A6J87_08680	1806944..1807027	+	84	ARC49421	23S rRNA methyltransferase attenuator leader peptide ErmL	-
A6J87_08685	1807152..1807889	+	738	ARC49422	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
A6J87_10830	1807894..1808025	+	132	AVH84132	hypothetical protein	-

Host bacterium

• ID: 276
• Element type: ICE (Integrative and conjugative element)
• Element name: ICE_SsaFDA259_Tn916
• GenBank: CP020451
• Element size: 2259318 bp
• Coordinate of oriT [Strand]: 2501..2633 [+]
• Host bacterium: Streptococcus salivarius strain FDAARGOS_259
• Coordinate of element: 1792004..1815306

Cargo genes

• Drug resistance gene: tet(M), erm(B)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -