Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200306
Name   oriT_ICE_Seq35246_TnGBS2 in_silico
Organism   Streptococcus equi subsp. zooepidemicus ATCC 35246
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_017582 (5428..5460 [-], 33 nt)
oriT length   33 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 33 nt

>oriT_ICE_Seq35246_TnGBS2
GCATGTGTGGCCACACGTGCTACGCTTGCCAGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14346 GenBank   WP_038673973
Name   Relaxase_SESEC_RS08945_ICE_Seq35246_TnGBS2 insolico UniProt ID   _
Length   547 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 547 a.a.        Molecular weight: 63939.19 Da        Isoelectric Point: 6.6451

>WP_038673973.1 relaxase/mobilization nuclease domain-containing protein [Streptococcus equi]
MVVTKVFQIKQTKNLKRAIDYITRDDATLIKVEQHDKDDAFDYTLTTDGEVHKKLISGHHLIDPGDRDAI
YDDFILLKQSVDDFYDNDTLSDLKNDNRVLAHHIVQSFSPDDNLTPEEVNEIGRKTALEFTGGDYQFVVA
THMDKGFLHNHIIFNTTNEVTLKKFRWQKGTKKSLEHISDKHAELYGAQILKPKLRTSYTDYSAWRRQNN
FSYEIKQRLNFLLKHSISVEDFQQKAKALDLHIDFSGKFTKYRLLVPLDGKLQEKNTRDRTLSKKRIYSL
EQIQKRVAMNEVVYTVDHIKEKYEEEQAKKAEDFEMKVRIEPWQISEITTQSIHLPVAFGLEQKGTVSIP
ARMLDQNEDGSFTAYFKKKDYFYFINPDKSTDNRFIQGGTLVKQLSLNSGEVILTKNWEMTELERLVEEF
NFLSANKVTNSKQFQEMQERFLEQLEKTDQTLAQLDSKVAYLNKVLGALSDYQDGLVPSEVTMAILEKAK
VAQNTDRNLIRKEIKELQIEREALQEARDRIVKDYDFANEMEQSYDHKHHQSKGHQR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(82-287)


  Protein structure



No available structure.




T4CP

ID   16838 GenBank   WP_038673967
Name   t4cp2_SESEC_RS08970_ICE_Seq35246_TnGBS2 insolico UniProt ID   _
Length   681 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 681 a.a.        Molecular weight: 78455.05 Da        Isoelectric Point: 4.9872

>WP_038673967.1 VirD4-like conjugal transfer protein, CD1115 family [Streptococcus equi]
MELKHKRLLPYLLLSLLLAYLTHRAYVLYWMAPQTDMTNLFAPYTYVFEKITEPPYFYWNTSPLAILSAL
VGFFVGLLFYLKIRLDGNYRHGEESGSARMATAQELKGFQDEEPRNNMIFSREAQMGLFNKRLPFERQLN
KNVLVAGLPGDGKTYNYVKPNLMQMNSSFIVTDPKGVLVREVGTLLKENGYQIKIFDLVNLTNSDMFNPF
KYMTSELDIDRITEAIVEGTKKGDREGEDFWLQAKLLLNRALLGYLYFDSQVRHYEPNLSMVADLLRNLK
RPNEKELSAVEKMFKQLEKSKPGNYACRQWELFNSNFEAETRTSVLAIVATQYSIFDHDAVTNLIKTDTM
EMDTWNTEKTAVFVAISETNKAYSFLASTFFTVVFDQLTHSADAIIQGEKKGYELEDLLHVQFIFDEFAN
CGKIPHFNEVLASIRSREMSIKIIIQAISQLDTLYGVPARKSMVNNCATLLFLGTNDEDTMKYFSMRAGK
QTITQTSYSEQRGQRRTGTTSHQAHQRDLMTPDEIARIGVDEALVFISKQNVFRDKKAMVSNHPMKDWLA
NHYQDKTWYHYKRFMEEGAEFIDAVMTGQIPEDHIWAPDMSDYQAFLEENGFDNTRQSAPETSVHDQVPV
TNPEMPSESPSQTPETSKKLVDMDTGEIFELPPEDREDFGEISFEDQRVEV

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(141-549)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1893966..1909275

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SESEC_RS08970 (SeseC_02291) 1891921..1893966 - 2046 WP_038673967 VirD4-like conjugal transfer protein, CD1115 family -
SESEC_RS08975 (SeseC_02292) 1893966..1894457 - 492 WP_014623487 DUF3801 domain-containing protein cd411
SESEC_RS08980 (SeseC_02293) 1894479..1895261 - 783 WP_014623488 hypothetical protein -
SESEC_RS08985 (SeseC_02294) 1895261..1895533 - 273 WP_014623489 hypothetical protein -
SESEC_RS08990 (SeseC_02295) 1895555..1896157 - 603 WP_038673964 hypothetical protein prgL
SESEC_RS08995 (SeseC_02296) 1896181..1898877 - 2697 WP_014623491 phage tail tip lysozyme prgK
SESEC_RS10695 (SeseC_02297) 1898910..1899440 + 531 WP_154224853 hypothetical protein -
SESEC_RS09010 (SeseC_02298) 1899634..1901985 - 2352 WP_014623493 VirB4-like conjugal transfer ATPase, CD1110 family virb4
SESEC_RS09015 (SeseC_02299) 1901966..1902325 - 360 WP_037582122 hypothetical protein -
SESEC_RS10970 (SeseC_02300) 1902681..1902914 - 234 WP_014623495 hypothetical protein -
SESEC_RS09025 (SeseC_02301) 1903075..1903827 - 753 WP_014623496 hypothetical protein prgHb
SESEC_RS09030 (SeseC_02302) 1903876..1904442 - 567 WP_014623497 hypothetical protein -
SESEC_RS09035 (SeseC_02303) 1904547..1904774 - 228 WP_000447009 hypothetical protein -
SESEC_RS09040 (SeseC_02304) 1905051..1908572 - 3522 WP_014623499 SspB-related isopeptide-forming adhesin prgB
SESEC_RS09045 (SeseC_02305) 1908637..1909275 - 639 WP_038673957 LPXTG cell wall anchor domain-containing protein prgC
SESEC_RS09050 (SeseC_02306) 1909290..1909589 - 300 WP_038673954 hypothetical protein -
SESEC_RS09055 (SeseC_02309) 1909957..1912242 - 2286 WP_038673952 C39 family peptidase -
SESEC_RS09060 (SeseC_02310) 1912280..1912606 - 327 WP_014623503 single-stranded DNA-binding protein -
SESEC_RS09065 (SeseC_02311) 1912603..1913691 - 1089 WP_014623504 replication initiator protein A -
SESEC_RS09070 (SeseC_02312) 1913692..1913877 - 186 WP_014623505 hypothetical protein -
SESEC_RS09075 (SeseC_02314) 1913946..1914233 - 288 Protein_1764 hypothetical protein -

Host bacterium

ID   274 Element type   ICE (Integrative and conjugative element)
Element name   ICE_Seq35246_TnGBS2 GenBank   NC_017582
Element size   2167264 bp Coordinate of oriT [Strand]   5428..5460 [-]
Host bacterium   Streptococcus equi subsp. zooepidemicus ATCC 35246 Coordinate of element   1882597..1914385

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21