Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200304
Name   oriT_ICE_FprA2-165_rumA in_silico
Organism   Faecalibacterium duncaniae strain JCM 31915
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   CP048437 (33130..33157 [+], 28 nt)
oriT length   28 nt
IRs (inverted repeats)      1..7, 12..18  (GGGAGTG..CACTCCC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 28 nt

>oriT_ICE_FprA2-165_rumA
GGGAGTGGCTACACTCCCTATGCTTGCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14342 GenBank   QIA43153
Name   Relaxase_GXM22_08770_ICE_FprA2-165_rumA insolico UniProt ID   _
Length   442 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 442 a.a.        Molecular weight: 50761.82 Da        Isoelectric Point: 9.4661

>QIA43153.1 relaxase/mobilization nuclease domain-containing protein [Faecalibacterium duncaniae]
MAVTKIHPIKSTLKKALDYIENPVKTDEKILVSSFACSYETADIEFELLLSQAMQKGNNLAHHLIQSFAP
GETTPEQAHEIGRQLADEVLQGKYPYVLTTHIDKGHVHNHIIFCAVDLVNQRKYVSNRQSYSYIRRTSDR
LCKEHNLSVVMPGQDRGKSYAEWNAHRKGTSWKAKLKAAIDTTIPQAKDFDDFLRLLQEQSYEVKRGKYV
SFRAPGQERFTRCKTLGEAYTEEAITERIKGRFAERKPKETRKISLQIDLENSIKAQQSAGYEKWAKLHN
LKQAARTLNFLTEHEIDSYPDLESRVAEITAVSTEAAAALKTAERRLAEMAVLIKDVTTCKELRPLVQEY
QQAADKKQFRRKHEGTLILYEAAAKALKEQGFQKLPDLYALKAEYKQLAEQKDQLQRQYNDAKRQMQEYG
IIKQNVDGILRTAPGKEQMQER

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(10-240)


  Protein structure



No available structure.



ID   14343 GenBank   QIA44352
Name   MobA_MobL_GXM22_08825_ICE_FprA2-165_rumA insolico UniProt ID   _
Length   471 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 471 a.a.        Molecular weight: 54978.44 Da        Isoelectric Point: 8.5444

>QIA44352.1 MobA/MobL family protein [Faecalibacterium duncaniae]
MASAAYRAGERLYSSYYGEVSDYTKKGGVIASEIMLPPHAPEIYLDRATLWNAVENCEKHPKAQLAYSFD
IAMQNELTLEENMELARKFVQEQFVTKGMIADLAFHSPEKEDGGIPNPHFHVMTTMRPLNPDGTWGQKQR
REYLLDEDGNRIRDKNGDYMFNAVHTTDWHEPETLEHWREQWAAAVNTKFEEKGLDVRIDHRSYVRQGLD
LIPTVHEGANVRQMEAKGIRTEKGELNRWIKATNRLMQDVRKKIKALFVWMAEVKEELSKPQTPSLADLL
IAYYNQRNAGAWSNKARTGNLKQFAEAVNYLTENKLLTLEDLQERLSSVNEEFEALSDSMKKKSARIKEL
QELIREGENYQRLKPVHTELNNIKFKKQREKFETSHDAELRLFYAARRILKEKLDGKPIALKAWKQEYAQ
LKTEYAELSPQHKPLREEVIRLRQVQNAVDTALRRREQPQAVQRKKHEMEL

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(2-227)


  Protein structure



No available structure.




Auxiliary protein

ID   7872 GenBank   QIA43154
Name   QIA43154_ICE_FprA2-165_rumA insolico UniProt ID   _
Length   109 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 109 a.a.        Molecular weight: 12619.71 Da        Isoelectric Point: 11.0031

>QIA43154.1 plasmid mobilization relaxosome protein MobC [Faecalibacterium duncaniae]
MTNRTRKIVLRVPVTPEERELIRQKMALLHTRNFSAYARKMLIDGYVVHIDTTGIREQTAELQKIGVNVN
QIARRLNSMGPLYTQDVADIKGALAQIWQLQRYILSSQR

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(60-99)


  Protein structure



No available structure.




T4CP

ID   16835 GenBank   QIA43128
Name   t4cp2_GXM22_08620_ICE_FprA2-165_rumA insolico UniProt ID   _
Length   601 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 601 a.a.        Molecular weight: 67732.63 Da        Isoelectric Point: 6.7002

>QIA43128.1 type IV secretory system conjugative DNA transfer family protein [Faecalibacterium duncaniae]
MKPELKKLLVLNLPYLLFVYLFAKCGQAYRLAAGADASAKLLHLTGGISVAFASPLPSLHLFDLCVGVVG
AVAVRLIVYSKGKNAKKYRKGEEYGSARWGTAKDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNK
NVLVIGGSGSGKTRFYVKPNLMQCFPTSDYPTSFVVTDPKGTLVLETGQMFQRAGYRVKILNTINFSKSM
KYNPFVYIHSEKDVLKLVNTLIANTKGEGEKSAEDFWVKSERLFYTALIGYIWYEAPAEEMNFTTLLEMI
NASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISCGARLAPFDIREVRELM
EDDEMELDTIGDEKTVLFLIMSDTDTTFNFILAMLQSQLINLLCDRADDKYGGRLPVHVRLILDEFANIG
QIPNFDKLIATIRSREISASIILQSQSQLKAIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETID
SFNTSENRGSQTSHGLNYQKLGKALMSEDEIAIMDGGRCILQLRGVRPFFSEKFDITKHPHYKYLADADK
KNTFDVDRFLSSLRRKRQQVVAQDESFDLYEIDLSDENAAE

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(140-543)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1762683..1784231

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXM22_08600 1759175..1760038 + 864 QIA43124 AraC family transcriptional regulator -
GXM22_08605 1760067..1761440 + 1374 QIA43125 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
GXM22_08610 1761668..1762582 + 915 QIA43126 helix-turn-helix domain-containing protein -
GXM22_08615 1762683..1763153 + 471 QIA43127 PcfB family protein cd411
GXM22_08620 1763150..1764955 + 1806 QIA43128 type IV secretory system conjugative DNA transfer family protein -
GXM22_08625 1765003..1765143 + 141 QIA43129 hypothetical protein prgF
GXM22_08630 1765348..1765725 + 378 QIA43130 GntR family transcriptional regulator -
GXM22_08635 1765728..1766588 + 861 QIA43131 ABC transporter ATP-binding protein -
GXM22_08640 1766585..1767232 + 648 QIA43132 ABC-2 transporter permease -
GXM22_08645 1767573..1768016 + 444 QIA43133 sigma-70 family RNA polymerase sigma factor -
GXM22_08650 1768009..1768209 + 201 QIA43134 helix-turn-helix domain-containing protein -
GXM22_08655 1768676..1769101 + 426 QIA44349 plasmid mobilization relaxosome protein MobC -
GXM22_08660 1769160..1770693 + 1534 Protein_1688 relaxase/mobilization nuclease domain-containing protein -
GXM22_08665 1770867..1771061 + 195 QIA44350 hypothetical protein -
GXM22_08670 1771051..1771791 + 741 QIA43135 replication protein -
GXM22_08675 1771788..1772627 + 840 QIA43136 ATP-binding protein -
GXM22_08680 1772640..1772831 + 192 QIA43137 hypothetical protein -
GXM22_08685 1772966..1774573 + 1608 QIA43138 DUF4368 domain-containing protein -
GXM22_08690 1774592..1774756 + 165 Protein_1694 hypothetical protein -
GXM22_08695 1774860..1775525 + 666 QIA43139 hypothetical protein prgHa
GXM22_08700 1776101..1777933 + 1833 QIA43140 group II intron reverse transcriptase/maturase -
GXM22_08705 1778009..1778311 + 303 QIA43141 hypothetical protein prgHa
GXM22_08710 1778332..1778757 + 426 QIA43142 PrgI family protein prgIa
GXM22_08715 1778684..1781086 + 2403 QIA43143 PrgI family protein virb4
GXM22_08720 1781083..1781979 + 897 QIA43144 DNA (cytosine-5-)-methyltransferase -
GXM22_08725 1781976..1783961 + 1986 QIA43145 C40 family peptidase cd419a
GXM22_08730 1783980..1784231 + 252 QIA43146 DUF4315 family protein cd419
GXM22_08735 1784221..1784943 + 723 QIA43147 DUF4366 domain-containing protein -
GXM22_08740 1784940..1787063 + 2124 QIA43148 DNA topoisomerase III -

Region 2: 2998030..3009698

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXM22_14455 2994438..2995241 - 804 QIA44164 phosphoadenosine phosphosulfate reductase -
GXM22_14460 2995192..2997261 - 2070 QIA44165 DNA topoisomerase III -
GXM22_14465 2997258..2998037 - 780 QIA44166 DUF4366 domain-containing protein -
GXM22_14470 2998030..2998266 - 237 QIA44167 DUF4315 family protein cd419
GXM22_14475 2998283..3000490 - 2208 QIA44168 peptidase M23 cd419a
GXM22_14480 3000490..3001443 - 954 QIA44410 site-specific DNA-methyltransferase -
GXM22_14485 3001472..3002047 - 576 QIA44169 nitrogen fixation protein -
GXM22_14490 3002063..3004462 - 2400 QIA44411 conjugal transfer protein TraE virb4
GXM22_14495 3004464..3004844 - 381 QIA44170 PrgI family protein prgIa
GXM22_14500 3004813..3005301 - 489 QIA44171 hypothetical protein -
GXM22_14505 3005289..3005882 - 594 QIA44172 adenine methyltransferase -
GXM22_14510 3005872..3006741 - 870 QIA44173 hypothetical protein prgHa
GXM22_14515 3006781..3006996 - 216 QIA44174 conjugal transfer protein prgF
GXM22_14520 3007091..3007306 - 216 QIA44175 conjugal transfer protein prgF
GXM22_14525 3007441..3009210 - 1770 QIA44176 type IV secretory system conjugative DNA transfer family protein virb4
GXM22_14530 3009207..3009698 - 492 QIA44177 PcfB family protein cd411
GXM22_14535 3009829..3010734 - 906 QIA44178 helix-turn-helix domain-containing protein -
GXM22_14540 3010731..3011504 - 774 QIA44179 toxin Bro -
GXM22_14545 3011497..3011745 - 249 QIA44180 hypothetical protein -
GXM22_14550 3011872..3012180 - 309 QIA44181 thiamine transporter -
GXM22_14555 3012334..3012549 - 216 Protein_2833 recombinase family protein -
GXM22_14560 3012695..3012889 - 195 QIA44182 hypothetical protein -
GXM22_14565 3012962..3013906 - 945 QIA44183 plasmid recombination protein -
GXM22_14570 3013878..3014065 - 188 Protein_2836 hypothetical protein -
GXM22_14575 3014090..3014485 - 396 QIA44184 replication initiator protein A -

Host bacterium

ID   272 Element type   ICE (Integrative and conjugative element)
Element name   ICE_FprA2-165_rumA GenBank   CP048437
Element size   3102523 bp Coordinate of oriT [Strand]   33130..33157 [+]
Host bacterium   Faecalibacterium duncaniae strain JCM 31915 Coordinate of element   1761411..1808878

Cargo genes

Drug resistance gene   ant(6)-Ia
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21, AcrIIA9