Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200303 |
| Name | oriT_ICE_erm42  |
| Organism | Salmonella enterica subsp. enterica serovar Albany strain R17.5974 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP060730 (3939..4237 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 234..242, 254..262 (AAAGCCAAA..TTTGGCTTT) 45..50, 52..57 (CAAACG..CGTTTG) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICE_erm42
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14341 | GenBank | QNN38204 |
| Name | TraI_2_H9S94_02855_ICE_erm42 |
UniProt ID | _ |
| Length | 715 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 715 a.a. Molecular weight: 79549.66 Da Isoelectric Point: 5.2787
>QNN38204.1 TraI domain-containing protein [Salmonella enterica subsp. enterica serovar Albany]
MFKNLFFQAKALPELSSQLDADIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQREFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKGLEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWLRKSNQSTHLCLVWKSAAKDIIDLLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSSVALFSKSEWEARKQTQAEPQSRSSEQPGL
PEESSSIELSNSAESPSTNSSEQDDELRHASDVNHLQANENVPGDGCEKPHHSSDAAIANNAKQHDAEAL
NLPESLAWLPEASSALVMVGEQILIRYPDAIRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNEGV
QEQGLLLKVSISKGLTVLIDLSNQDEEPAEIQDEVAAQRPSRINSTNAQAKEPVTRAERKQKPIAPNANS
STDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQCQ
FDEGETVLFTAHAKR
MFKNLFFQAKALPELSSQLDADIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQREFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKGLEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWLRKSNQSTHLCLVWKSAAKDIIDLLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSSVALFSKSEWEARKQTQAEPQSRSSEQPGL
PEESSSIELSNSAESPSTNSSEQDDELRHASDVNHLQANENVPGDGCEKPHHSSDAAIANNAKQHDAEAL
NLPESLAWLPEASSALVMVGEQILIRYPDAIRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNEGV
QEQGLLLKVSISKGLTVLIDLSNQDEEPAEIQDEVAAQRPSRINSTNAQAKEPVTRAERKQKPIAPNANS
STDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQCQ
FDEGETVLFTAHAKR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16833 | GenBank | QNN38205 |
| Name | traD_H9S94_02860_ICE_erm42 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 66996.79 Da Isoelectric Point: 6.6970
>QNN38205.1 conjugative transfer system coupling protein TraD [Salmonella enterica subsp. enterica serovar Albany]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEVLTELPPEPFWWMTGISSGMALYRLPEAYRLHKLQKG
LKGKPLAFMALSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEVLTELPPEPFWWMTGISSGMALYRLPEAYRLHKLQKG
LKGKPLAFMALSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16834 | GenBank | QNN38219 |
| Name | traC_H9S94_02935_ICE_erm42 |
UniProt ID | _ |
| Length | 799 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 799 a.a. Molecular weight: 90128.77 Da Isoelectric Point: 5.9735
>QNN38219.1 type IV secretion system protein TraC [Salmonella enterica subsp. enterica serovar Albany]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHLQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTKGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHLQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTKGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 606689..627902
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H9S94_02850 | 603155..604336 | + | 1182 | QNN38203 | DUF4268 domain-containing protein | - |
| H9S94_02855 | 604493..606640 | + | 2148 | QNN38204 | TraI domain-containing protein | - |
| H9S94_02860 | 606689..608509 | + | 1821 | QNN38205 | conjugative transfer system coupling protein TraD | virb4 |
| H9S94_02865 | 608519..609079 | + | 561 | QNN38206 | conjugative transfer protein | - |
| H9S94_02870 | 609066..609701 | + | 636 | QNN38207 | DUF4400 domain-containing protein | tfc7 |
| H9S94_02875 | 609815..610411 | + | 597 | QNN38208 | hypothetical protein | - |
| H9S94_02880 | 610404..611510 | + | 1107 | QNN38209 | ImmA/IrrE family metallo-endopeptidase | - |
| H9S94_02885 | 611616..612656 | + | 1041 | QNN38210 | IS481 family transposase | - |
| H9S94_02890 | 612853..613134 | + | 282 | QNN38211 | type IV conjugative transfer system protein TraL | traL |
| H9S94_02895 | 613131..613757 | + | 627 | QNN38212 | pilus assembly protein | traE |
| H9S94_02900 | 613741..614637 | + | 897 | QNN39910 | type-F conjugative transfer system secretin TraK | traK |
| H9S94_02905 | 614640..615929 | + | 1290 | QNN38213 | TraB/VirB10 family protein | traB |
| H9S94_02910 | 616004..616576 | + | 573 | QNN38214 | type IV conjugative transfer system lipoprotein TraV | traV |
| H9S94_02915 | 616573..616959 | + | 387 | QNN38215 | hypothetical protein | - |
| H9S94_02920 | 617000..617506 | - | 507 | QNN38216 | GNAT family N-acetyltransferase | - |
| H9S94_02925 | 617497..617763 | - | 267 | QNN38217 | DUF1778 domain-containing protein | - |
| H9S94_02930 | 618133..618825 | + | 693 | QNN38218 | DsbC family protein | trbB |
| H9S94_02935 | 618825..621224 | + | 2400 | QNN38219 | type IV secretion system protein TraC | virb4 |
| H9S94_02940 | 621217..621564 | + | 348 | QNN38220 | plasmid-related protein | - |
| H9S94_02945 | 621548..622060 | + | 513 | QNN38221 | S26 family signal peptidase | - |
| H9S94_02950 | 622071..623195 | + | 1125 | QNN39911 | pilus assembly protein | traW |
| H9S94_02955 | 623179..624207 | + | 1029 | QNN38222 | TraU family protein | traU |
| H9S94_02960 | 624210..627902 | + | 3693 | QNN38223 | conjugal transfer protein TraN | traN |
| H9S94_02965 | 628134..628466 | + | 333 | QNN38224 | hypothetical protein | - |
| H9S94_02970 | 628497..629159 | + | 663 | QNN38225 | hypothetical protein | - |
| H9S94_02975 | 629250..629852 | - | 603 | QNN38226 | hypothetical protein | - |
| H9S94_02980 | 630220..630546 | + | 327 | QNN38227 | plasmid-related protein | - |
| H9S94_02985 | 630562..630981 | + | 420 | QNN38228 | single-stranded DNA-binding protein | - |
| H9S94_02990 | 631061..631879 | + | 819 | QNN38229 | phage recombination protein Bet | - |
| H9S94_02995 | 631961..632104 | + | 144 | QNN38230 | hypothetical protein | - |
Host bacterium
| ID | 271 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICE_erm42 | GenBank | CP060730 |
| Element size | 5024703 bp | Coordinate of oriT [Strand] | 3939..4237 [+] |
| Host bacterium | Salmonella enterica subsp. enterica serovar Albany strain R17.5974 | Coordinate of element | 568141..660867 |
Cargo genes
| Drug resistance gene | erm(42), floR, sul2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |