Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200295
• Name: oriT1_ICEKpnWCHKP7E2-2
• Organism: Klebsiella pneumoniae strain WCHKP7E2
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP028806 (9425..9457 [-], 33 nt)
• oriT length: 33 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 33 nt

>oriT1_ICEKpnWCHKP7E2-2
GGCTTAGCCAGTTTTCCGTATCCTGACACCTCC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Replic_Relax_B7D49_12205_ICEKpnWCHKP7E2-2

• ID: 14333
• GenBank: AWA67884
• Name: Replic_Relax_B7D49_12205_ICEKpnWCHKP7E2-2
• UniProt ID: _
• Length: 248 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 248 a.a.
• Molecular weight: 29203.78 Da
• Isoelectric Point: 10.3502

>AWA67884.2 molybdopterin-guanine dinucleotide biosynthesis protein MobC [Klebsiella pneumoniae]
MLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESRTI
KISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLSQY
QVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLFDS
IRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT

Protein domains

No domain identified.

Protein structure

No available structure.

T4CP

t4cp2_B7D49_12210_ICEKpnWCHKP7E2-2

• ID: 16824
• GenBank: AWA67885
• Name: t4cp2_B7D49_12210_ICEKpnWCHKP7E2-2
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69429.53 Da
• Isoelectric Point: 6.2155

>AWA67885.1 TraM recognition domain-containing protein [Klebsiella pneumoniae]
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF

Protein domains

No domain identified.

Protein structure

No available structure.

t4cp2_B7D49_12275_ICEKpnWCHKP7E2-2

• ID: 16825
• GenBank: AWA67897
• Name: t4cp2_B7D49_12275_ICEKpnWCHKP7E2-2
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103120.09 Da
• Isoelectric Point: 7.1738

>AWA67897.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTIFPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1827027..1836967

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D49_12205	1822614..1823360	-	747	AWA67884	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
B7D49_12210	1823370..1825229	-	1860	AWA67885	TraM recognition domain-containing protein	-
B7D49_12215	1825247..1825507	-	261	AWA67886	hypothetical protein	-
B7D49_12220	1825526..1825918	-	393	AWA67887	hypothetical protein	-
B7D49_12225	1825939..1826244	-	306	AWA67888	dpoa decarboxylase	-
B7D49_12230	1826289..1826591	-	303	AWA67889	kikA from plasmid origin	-
B7D49_12235	1826627..1827030	-	404	Protein_1761	Cag pathogenicity island protein Cag12	-
B7D49_12240	1827027..1828052	-	1026	AWA67890	P-type DNA transfer ATPase VirB11	virB11
B7D49_12245	1828042..1829304	-	1263	AWA67891	type VI secretion protein	virB10
B7D49_12250	1829348..1830253	-	906	AWA67892	P-type conjugative transfer protein VirB9	virB9
B7D49_12255	1830253..1830936	-	684	AWA67893	type IV secretion system protein	virB8
B7D49_12260	1831154..1832227	-	1074	AWA67894	type IV secretion system protein	virB6
B7D49_12265	1832231..1832473	-	243	AWA67895	EexN family lipoprotein	-
B7D49_12270	1832481..1833194	-	714	AWA67896	type IV secretion system protein VirB5	-
B7D49_12275	1833213..1835951	-	2739	AWA67897	ATPase	virb4
B7D49_12280	1835964..1836257	-	294	AWA67898	TrbC/VirB2 family protein	virB2
B7D49_12285	1836257..1836967	-	711	AWA67899	lytic transglycosylase domain-containing protein	virB1
B7D49_12290	1837040..1837273	-	234	AWA67900	hypothetical protein	-
B7D49_12295	1837842..1838009	-	168	AWA67901	transcriptional regulator	-
B7D49_12300	1838213..1838422	-	210	AWA67902	TraR/DksA family transcriptional regulator	-
B7D49_12305	1838412..1838993	-	582	AWA67903	DUF2857 domain-containing protein	-
B7D49_12310	1838978..1839163	-	186	AWA67904	hypothetical protein	-
B7D49_12315	1839182..1839367	-	186	AWA67905	AlpA family transcriptional regulator	-
B7D49_30350	1839507..1840061	-	555	QEM38984	hypothetical protein	-
B7D49_30355	1840077..1840490	-	414	Protein_1779	hypothetical protein	-

Host bacterium

• ID: 267
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnWCHKP7E2-2
• GenBank: CP028806
• Element size: 5371030 bp
• Coordinate of oriT [Strand]: 9425..9457 [-]; 19035..19158 [-]
• Host bacterium: Klebsiella pneumoniae strain WCHKP7E2
• Coordinate of element: 1806441..1871766

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -