Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200292 |
| Name | oriT_ICEKpnWCHKP13F2-1  |
| Organism | Klebsiella pneumoniae strain WCHKP13F2 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP028391 (56789..56912 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnWCHKP13F2-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16820 | GenBank | AVW76041 |
| Name | t4cp2_B7D34_11305_ICEKpnWCHKP13F2-1 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>AVW76041.1 type IV secretion system DNA-binding domain-containing protein [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16821 | GenBank | AVW76055 |
| Name | t4cp2_B7D34_11375_ICEKpnWCHKP13F2-1 |
UniProt ID | _ |
| Length | 914 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 914 a.a. Molecular weight: 103265.39 Da Isoelectric Point: 7.7540
>AVW76055.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIIAVWTSVALLFLLPVQFLVMKSLTRNEPMRFSLIAVWLR
AKGNPVANRLFGATTFMPVEHDAVDIREFLDTMKLNQCATIRKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDETLRFGANQLNGLIRSFEGMPVTFYVHNSREQFTDSLHRISGNAYADTISELYYTSLGKRP
FRRNRLFLTVCYTPFVSLEKAERKRMKDGQKLKELDGALLEMLEIKSSLDTALARYGACPLGTFSEGEMV
FSSQLAFYEYLLTHQWRKVRITRTPACDVMGAAALFFSAESGQINHASGTQYFRGLEIKEFSEETATGMM
DSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLLSSDDDAVSQRLDLDVALDLLTSGKIAYGKHHFSI
MVYSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNFTLRPRKGALSSQNFVELAALHNF
YPGKRNNAPWGDAMALMRTPSGDGYYLNLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFLEIMQQK
YGREDSFSPDARTKRLTTVYLDKDRGAEMNIRALGGRYWRVISGEPTGWNPFSLPATKRNINFIKQLMKI
LCTRNGATITPRDERRLNDAVNAVMSDEPEYRRFGITRLREQLPEPATKEAQENGLDIRLSQWAQGGEFG
WVFDNESDTFDISHCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKD
FAYNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPSADRAHYVDGMKFEPEVFDVVK
GLDPQARQYVVVKNQFRRGDTKRFAARVTLDLSGVGRYTKVMSGDAPNLEIFESIYREGMRPHEWLDTYL
AKAL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIIAVWTSVALLFLLPVQFLVMKSLTRNEPMRFSLIAVWLR
AKGNPVANRLFGATTFMPVEHDAVDIREFLDTMKLNQCATIRKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDETLRFGANQLNGLIRSFEGMPVTFYVHNSREQFTDSLHRISGNAYADTISELYYTSLGKRP
FRRNRLFLTVCYTPFVSLEKAERKRMKDGQKLKELDGALLEMLEIKSSLDTALARYGACPLGTFSEGEMV
FSSQLAFYEYLLTHQWRKVRITRTPACDVMGAAALFFSAESGQINHASGTQYFRGLEIKEFSEETATGMM
DSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLLSSDDDAVSQRLDLDVALDLLTSGKIAYGKHHFSI
MVYSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNFTLRPRKGALSSQNFVELAALHNF
YPGKRNNAPWGDAMALMRTPSGDGYYLNLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFLEIMQQK
YGREDSFSPDARTKRLTTVYLDKDRGAEMNIRALGGRYWRVISGEPTGWNPFSLPATKRNINFIKQLMKI
LCTRNGATITPRDERRLNDAVNAVMSDEPEYRRFGITRLREQLPEPATKEAQENGLDIRLSQWAQGGEFG
WVFDNESDTFDISHCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKD
FAYNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPSADRAHYVDGMKFEPEVFDVVK
GLDPQARQYVVVKNQFRRGDTKRFAARVTLDLSGVGRYTKVMSGDAPNLEIFESIYREGMRPHEWLDTYL
AKAL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1823127..1836977
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| B7D34_11280 | 1818308..1819822 | + | 1515 | AVW76037 | precolibactin peptidase ClbP | - |
| B7D34_11285 | 1819815..1820537 | + | 723 | AVW76038 | colibactin biosynthesis thioesterase ClbQ | - |
| B7D34_11290 | 1820572..1821084 | + | 513 | AVW76039 | colibactin self-protection protein ClbS | - |
| B7D34_29220 | 1821069..1821272 | + | 204 | QEO60418 | hypothetical protein | - |
| B7D34_11295 | 1822280..1822378 | - | 99 | Protein_1724 | nuclease | - |
| B7D34_11300 | 1822354..1823118 | - | 765 | AVW76040 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| B7D34_11305 | 1823127..1824866 | - | 1740 | AVW76041 | type IV secretion system DNA-binding domain-containing protein | virb4 |
| B7D34_11310 | 1824957..1825211 | - | 255 | AVW76042 | hypothetical protein | - |
| B7D34_11315 | 1825230..1825622 | - | 393 | AVW76043 | hypothetical protein | - |
| B7D34_11320 | 1825644..1825949 | - | 306 | AVW76044 | dpoa decarboxylase | - |
| B7D34_11325 | 1825992..1826294 | - | 303 | AVW76045 | kikA from plasmid origin | - |
| B7D34_11330 | 1826328..1826726 | - | 399 | AVW76046 | Cag pathogenicity island protein Cag12 | - |
| B7D34_11335 | 1826723..1827748 | - | 1026 | AVW76047 | P-type DNA transfer ATPase VirB11 | virB11 |
| B7D34_11340 | 1827738..1828058 | - | 321 | AVW76048 | hypothetical protein | - |
| B7D34_11345 | 1828070..1829311 | - | 1242 | AVW76049 | TrbI/VirB10 family protein | virB10 |
| B7D34_11350 | 1829355..1830260 | - | 906 | AVW76050 | P-type conjugative transfer protein VirB9 | virB9 |
| B7D34_11355 | 1830260..1830943 | - | 684 | AVW76051 | type IV secretion system protein | virB8 |
| B7D34_11360 | 1831165..1832235 | - | 1071 | AVW76052 | type IV secretion system protein | virB6 |
| B7D34_11365 | 1832247..1832471 | - | 225 | AVW76053 | EexN family lipoprotein | - |
| B7D34_11370 | 1832483..1833190 | - | 708 | AVW76054 | type IV secretion system protein VirB5 | - |
| B7D34_11375 | 1833209..1835953 | - | 2745 | AVW76055 | ATPase | virb4 |
| B7D34_11380 | 1835965..1836270 | - | 306 | AVW76056 | TrbC/VirB2 family protein | virB2 |
| B7D34_11385 | 1836267..1836977 | - | 711 | AVW76057 | lytic transglycosylase domain-containing protein | virB1 |
| B7D34_11390 | 1837872..1838081 | - | 210 | AVW76058 | TraR/DksA family transcriptional regulator | - |
| B7D34_11395 | 1838071..1838655 | - | 585 | AVW76059 | DUF2857 domain-containing protein | - |
| B7D34_11400 | 1838844..1839029 | - | 186 | AVW76060 | AlpA family transcriptional regulator | - |
| B7D34_11405 | 1839171..1840097 | - | 927 | AVW76061 | hypothetical protein | - |
| B7D34_11410 | 1840567..1841196 | + | 630 | AVW76062 | LuxR family transcriptional regulator | - |
Region 2: 1884820..1894050
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| B7D34_11630 | 1880408..1881154 | - | 747 | AVW76099 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| B7D34_11635 | 1881164..1883023 | - | 1860 | AVW76100 | TraM recognition domain-containing protein | - |
| B7D34_11640 | 1883041..1883301 | - | 261 | AVW76101 | hypothetical protein | - |
| B7D34_11645 | 1883320..1883712 | - | 393 | AVW76102 | hypothetical protein | - |
| B7D34_11650 | 1883733..1884038 | - | 306 | AVW76103 | dpoa decarboxylase | - |
| B7D34_11655 | 1884083..1884385 | - | 303 | AVW76104 | kikA from plasmid origin | - |
| B7D34_11660 | 1884421..1884823 | - | 403 | Protein_1792 | Cag pathogenicity island protein Cag12 | - |
| B7D34_11665 | 1884820..1885845 | - | 1026 | AVW76105 | P-type DNA transfer ATPase VirB11 | virB11 |
| B7D34_11670 | 1885835..1887097 | - | 1263 | AVW76106 | type VI secretion protein | virB10 |
| B7D34_11675 | 1887141..1888046 | - | 906 | AVW76107 | P-type conjugative transfer protein VirB9 | virB9 |
| B7D34_11680 | 1888046..1888729 | - | 684 | AVW76108 | type IV secretion system protein | virB8 |
| B7D34_11685 | 1888947..1890020 | - | 1074 | AVW76109 | type IV secretion system protein | virB6 |
| B7D34_11690 | 1890024..1890266 | - | 243 | AVW76110 | EexN family lipoprotein | - |
| B7D34_11695 | 1890274..1890987 | - | 714 | AVW76111 | type IV secretion system protein VirB5 | - |
| B7D34_11700 | 1891006..1893744 | - | 2739 | AVW76112 | ATPase | virb4 |
| B7D34_11705 | 1893757..1894050 | - | 294 | AVW76113 | TrbC/VirB2 family protein | virB2 |
| B7D34_11710 | 1894050..1894761 | - | 712 | Protein_1802 | lytic transglycosylase domain-containing protein | - |
| B7D34_11715 | 1894834..1895067 | - | 234 | AVW76114 | hypothetical protein | - |
| B7D34_11720 | 1895636..1895788 | - | 153 | AVW76115 | transcriptional regulator | - |
| B7D34_11725 | 1896007..1896216 | - | 210 | AVW76116 | TraR/DksA family transcriptional regulator | - |
| B7D34_11730 | 1896206..1896787 | - | 582 | AVW76117 | DUF2857 domain-containing protein | - |
| B7D34_11735 | 1896772..1896957 | - | 186 | AVW76118 | hypothetical protein | - |
| B7D34_11740 | 1896976..1897161 | - | 186 | AVW76119 | AlpA family transcriptional regulator | - |
| B7D34_29225 | 1897301..1897855 | - | 555 | QEO60441 | hypothetical protein | - |
| B7D34_29230 | 1897871..1898284 | - | 414 | Protein_1810 | hypothetical protein | - |
Host bacterium
| ID | 266 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnWCHKP13F2-1 | GenBank | CP028391 |
| Element size | 5375242 bp | Coordinate of oriT [Strand] | 56789..56912 [-] |
| Host bacterium | Klebsiella pneumoniae strain WCHKP13F2 | Coordinate of element | 1768391..1849138 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | clbA, clbB, clbC, clbD, clbE, clbF, clbG, clbH, clbI, clbJ, clbK, clbL, clbM, clbN, clbO, clbP, clbQ, clbS, fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |