Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200288
Name   oriT1_ICEKpnTK421-2 in_silico
Organism   Klebsiella pneumoniae strain TK421
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   CP045694 (6282..6314 [-], 33 nt)
oriT length   33 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 33 nt

>oriT1_ICEKpnTK421-2
GGCTTAGCCAGTTTTCCGTATCCTGACACCTCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14331 GenBank   QGA60848
Name   Replic_Relax_GHA50_10915_ICEKpnTK421-2 insolico UniProt ID   _
Length   248 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 248 a.a.        Molecular weight: 29203.78 Da        Isoelectric Point: 10.3502

>QGA60848.1 molybdopterin-guanine dinucleotide biosynthesis protein MobC [Klebsiella pneumoniae]
MLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESRTI
KISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLSQY
QVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLFDS
IRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT

  Protein domains



No domain identified.



  Protein structure



No available structure.




T4CP

ID   16814 GenBank   QGA60849
Name   t4cp2_GHA50_10920_ICEKpnTK421-2 insolico UniProt ID   _
Length   619 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 619 a.a.        Molecular weight: 69429.53 Da        Isoelectric Point: 6.2155

>QGA60849.1 TraM recognition domain-containing protein [Klebsiella pneumoniae]
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF

  Protein domains



No domain identified.


  Protein structure



No available structure.



ID   16815 GenBank   QGA60859
Name   t4cp2_GHA50_10975_ICEKpnTK421-2 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103059.99 Da        Isoelectric Point: 7.1738

>QGA60859.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

Type IV secretory pathway, VirB3-like protein (VirB3)

(3-79)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(269-470)

TraG P-loop domain (P-loop_TraG)

(535-813)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1949374..1959314

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHA50_10915 1944966..1945712 - 747 QGA60848 molybdopterin-guanine dinucleotide biosynthesis protein MobC -
GHA50_10920 1945722..1947581 - 1860 QGA60849 TraM recognition domain-containing protein -
GHA50_10925 1948291..1948596 - 306 QGA60850 dpoa decarboxylase -
GHA50_10930 1948641..1948943 - 303 QGA60851 kikA from plasmid origin -
GHA50_10935 1948979..1949377 - 399 QGA60852 Cag pathogenicity island protein Cag12 -
GHA50_10940 1949374..1950399 - 1026 QGA60853 P-type DNA transfer ATPase VirB11 virB11
GHA50_10945 1950389..1951651 - 1263 QGA60854 type VI secretion protein virB10
GHA50_10950 1951695..1952600 - 906 QGA63798 P-type conjugative transfer protein VirB9 virB9
GHA50_10955 1952600..1953283 - 684 QGA60855 type IV secretion system protein virB8
GHA50_10960 1953501..1954574 - 1074 QGA60856 conjugal transfer protein TrbL virB6
GHA50_10965 1954578..1954820 - 243 QGA60857 EexN family lipoprotein -
GHA50_10970 1954828..1955541 - 714 QGA60858 type IV secretion system protein VirB5 -
GHA50_10975 1955560..1958298 - 2739 QGA60859 ATPase virb4
GHA50_10980 1958311..1958604 - 294 QGA60860 TriB protein virB2
GHA50_10985 1958604..1959314 - 711 QGA60861 transglycosylase SLT domain-containing protein virB1
GHA50_10990 1959387..1959620 - 234 QGA60862 hypothetical protein -
GHA50_10995 1960189..1960356 - 168 QGA60863 hypothetical protein -
GHA50_11000 1960560..1960769 - 210 QGA60864 TraR/DksA family transcriptional regulator -
GHA50_11005 1960759..1961340 - 582 QGA60865 DUF2857 family protein -
GHA50_11010 1961325..1961510 - 186 QGA60866 hypothetical protein -
GHA50_11015 1961529..1961714 - 186 QGA60867 AlpA family phage regulatory protein -
GHA50_11020 1961854..1962408 - 555 QGA63799 hypothetical protein -
GHA50_11025 1962424..1962837 - 414 Protein_1838 hypothetical protein -

Host bacterium

ID   263 Element type   ICE (Integrative and conjugative element)
Element name   ICEKpnTK421-2 GenBank   CP045694
Element size   5277246 bp Coordinate of oriT [Strand]   6282..6314 [-]; 15892..16015 [-]
Host bacterium   Klebsiella pneumoniae strain TK421 Coordinate of element   1931936..1994113

Cargo genes

Drug resistance gene   -
Virulence gene   fyuA, ybtE, ybtT, ybtU, irp1, ybtA, ybtP, ybtQ, ybtX, ybtS
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -