Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200285 |
| Name | oriT2_ICEKpnST101:960186733-1  |
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain ST101:960186733 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP023487 ( 18259..18382 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT2_ICEKpnST101:960186733-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14329 | GenBank | ATO04998 |
| Name | Replic_Relax_AN663_11535_ICEKpnST101:960186733-1 |
UniProt ID | _ |
| Length | 250 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 250 a.a. Molecular weight: 29434.10 Da Isoelectric Point: 10.3502
>ATO04998.1 molybdopterin-guanine dinucleotide biosynthesis protein MobC [Klebsiella pneumoniae subsp. pneumoniae]
MVMLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESR
TIKISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLS
QYQVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLF
DSIRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT
MVMLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESR
TIKISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLS
QYQVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLF
DSIRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT
Protein domains
No domain identified.
Protein structure
No available structure.
T4CP
| ID | 16810 | GenBank | ATO04999 |
| Name | t4cp2_AN663_11540_ICEKpnST101:960186733-1 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69429.53 Da Isoelectric Point: 6.2155
>ATO04999.1 MFS transporter [Klebsiella pneumoniae subsp. pneumoniae]
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF
Protein domains
No domain identified.
Protein structure
No available structure.
| ID | 16811 | GenBank | ATO05011 |
| Name | t4cp2_AN663_11605_ICEKpnST101:960186733-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103120.09 Da Isoelectric Point: 7.1738
>ATO05011.1 ATPase [Klebsiella pneumoniae subsp. pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTIFPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTIFPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2327935..2337893
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AN663_11535 | 2323522..2324274 | - | 753 | ATO04998 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| AN663_11540 | 2324278..2326137 | - | 1860 | ATO04999 | MFS transporter | - |
| AN663_11545 | 2326155..2326415 | - | 261 | ATO05000 | hypothetical protein | - |
| AN663_11550 | 2326434..2326826 | - | 393 | ATO05001 | hypothetical protein | - |
| AN663_11555 | 2326847..2327152 | - | 306 | ATO05002 | dpoa decarboxylase | - |
| AN663_11560 | 2327197..2327499 | - | 303 | ATO05003 | kikA from plasmid origin | - |
| AN663_11565 | 2327535..2327938 | - | 404 | Protein_2243 | Cag pathogenicity island protein Cag12 | - |
| AN663_11570 | 2327935..2328960 | - | 1026 | ATO05004 | P-type DNA transfer ATPase VirB11 | virB11 |
| AN663_11575 | 2328950..2330218 | - | 1269 | ATO05005 | type VI secretion protein | virB10 |
| AN663_11580 | 2330256..2331164 | - | 909 | ATO05006 | P-type conjugative transfer protein VirB9 | virB9 |
| AN663_11585 | 2331161..2331844 | - | 684 | ATO05007 | hypothetical protein | virB8 |
| AN663_11590 | 2332062..2333135 | - | 1074 | ATO05008 | conjugal transfer protein TrbL | virB6 |
| AN663_11595 | 2333139..2333381 | - | 243 | ATO05009 | hypothetical protein | - |
| AN663_11600 | 2333389..2334102 | - | 714 | ATO05010 | type IV secretion system protein VirB5 | - |
| AN663_11605 | 2334121..2336859 | - | 2739 | ATO05011 | ATPase | virb4 |
| AN663_11610 | 2336872..2337165 | - | 294 | ATO05012 | TriB protein | virB2 |
| AN663_11615 | 2337165..2337893 | - | 729 | ATO05013 | type VI secretion protein | virB1 |
| AN663_11620 | 2337948..2338181 | - | 234 | ATO05014 | hypothetical protein | - |
| AN663_11625 | 2338750..2338917 | - | 168 | ATO05015 | transcriptional regulator | - |
| AN663_11630 | 2339121..2339330 | - | 210 | ATO05016 | conjugal transfer protein TraR | - |
| AN663_11635 | 2339320..2339901 | - | 582 | ATO05017 | DUF2857 domain-containing protein | - |
| AN663_11640 | 2339886..2340071 | - | 186 | ATO05018 | hypothetical protein | - |
| AN663_11645 | 2340090..2340275 | - | 186 | ATO05019 | transcriptional regulator | - |
| AN663_11650 | 2340415..2341398 | - | 984 | ATO05020 | hypothetical protein | - |
Host bacterium
| ID | 261 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnST101:960186733-1 | GenBank | CP023487 |
| Element size | 5498599 bp | Coordinate of oriT [Strand] | 8649..8681 [-]; 18259..18382 [-] |
| Host bacterium | Klebsiella pneumoniae subsp. pneumoniae strain ST101:960186733 | Coordinate of element | 2308125..2372672 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | fyuA, ybtE, ybtT, ybtU, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |