Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200283
• Name: oriT_ICEKpnSMU18037509-1
• Organism: Klebsiella pneumoniae strain SMU18037509
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP045661 (17179..17302 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnSMU18037509-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_GFJ44_08675_ICEKpnSMU18037509-1

• ID: 16808
• GenBank: QGA02410
• Name: t4cp2_GFJ44_08675_ICEKpnSMU18037509-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69595.85 Da
• Isoelectric Point: 6.2735

>QGA02410.1 TraM recognition domain-containing protein [Klebsiella pneumoniae]
MILNKLAASLSPIVNGMQALLAFVQQQQLMLALLAGLTIPFFASMKSDERQKAPLWKRLIIAFSLLCFLS
GTLAPVVIGFFQWLYKSRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELLVAGFSLA
EKGQESDFYRIEDRKAARMAAQFVSDDSSATIRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPKGF
SLKQMFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAEKRDRVTDVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAATVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (302-542)

Protein structure

No available structure.

t4cp2_GFJ44_08740_ICEKpnSMU18037509-1

• ID: 16809
• GenBank: QGA02421
• Name: t4cp2_GFJ44_08740_ICEKpnSMU18037509-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103084.02 Da
• Isoelectric Point: 7.4625

>QGA02421.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1794191..1804115

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFJ44_08670	1789791..1790537	-	747	QGA02409	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
GFJ44_08675	1790547..1792406	-	1860	QGA02410	TraM recognition domain-containing protein	-
GFJ44_08680	1792424..1792684	-	261	QGA02411	hypothetical protein	-
GFJ44_08685	1792703..1793005	-	303	QGA02412	hypothetical protein	-
GFJ44_08690	1793116..1793421	-	306	QGA02413	dpoa decarboxylase	-
GFJ44_08695	1793455..1793760	-	306	QGA02414	conjugal transfer protein	-
GFJ44_08700	1793796..1794194	-	399	QGA02415	Cag pathogenicity island protein Cag12	-
GFJ44_08705	1794191..1795216	-	1026	QGA02416	P-type DNA transfer ATPase VirB11	virB11
GFJ44_08710	1795206..1796456	-	1251	QGA02417	type VI secretion protein	virB10
GFJ44_08715	1796500..1797405	-	906	QGA05669	P-type conjugative transfer protein VirB9	virB9
GFJ44_08720	1797405..1798088	-	684	QGA02418	type IV secretion system protein	virB8
GFJ44_08725	1798310..1799383	-	1074	QGA02419	conjugal transfer protein TrbL	virB6
GFJ44_08730	1799387..1799623	-	237	QGA05670	EexN family lipoprotein	-
GFJ44_08735	1799636..1800343	-	708	QGA02420	type IV secretion system protein VirB5	-
GFJ44_08740	1800361..1803099	-	2739	QGA02421	ATPase	virb4
GFJ44_08745	1803112..1803405	-	294	QGA02422	TriB protein	virB2
GFJ44_08750	1803405..1804115	-	711	QGA02423	transglycosylase SLT domain-containing protein	virB1
GFJ44_08755	1804188..1804421	-	234	QGA02424	hypothetical protein	-
GFJ44_08760	1804990..1805157	-	168	QGA02425	hypothetical protein	-
GFJ44_08765	1805361..1805570	-	210	QGA02426	TraR/DksA family transcriptional regulator	-
GFJ44_08770	1805560..1806141	-	582	QGA02427	DUF2857 family protein	-
GFJ44_08775	1806126..1806311	-	186	QGA02428	hypothetical protein	-
GFJ44_08780	1806330..1806515	-	186	QGA02429	AlpA family phage regulatory protein	-
GFJ44_08785	1806655..1807209	-	555	QGA05671	hypothetical protein	-
GFJ44_08790	1807225..1807638	-	414	Protein_1720	hypothetical protein	-

Host bacterium

• ID: 260
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnSMU18037509-1
• GenBank: CP045661
• Element size: 5365096 bp
• Coordinate of oriT [Strand]: 17179..17302 [-]
• Host bacterium: Klebsiella pneumoniae strain SMU18037509
• Coordinate of element: 1775474..1838916

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -