Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200282
• Name: oriT_ICEKpnSKGH01-1
• Organism: Klebsiella pneumoniae strain SKGH01
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP015500 (11944..12067 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnSKGH01-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_A7B01_08255_ICEKpnSKGH01-1

• ID: 16806
• GenBank: ANE69619
• Name: t4cp2_A7B01_08255_ICEKpnSKGH01-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69574.86 Da
• Isoelectric Point: 6.6673

>ANE69619.1 molybdopterin-guanine dinucleotide biosynthesis protein MobB [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (418-542)

Protein structure

No available structure.

t4cp2_A7B01_08295_ICEKpnSKGH01-1

• ID: 16807
• GenBank: ANE69626
• Name: t4cp2_A7B01_08295_ICEKpnSKGH01-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103068.02 Da
• Isoelectric Point: 7.4625

>ANE69626.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1672389..1682306

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7B01_08250	1667990..1668736	-	747	ANE69618	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
A7B01_08255	1668746..1670605	-	1860	ANE69619	molybdopterin-guanine dinucleotide biosynthesis protein MobB	-
A7B01_08260	1671314..1671619	-	306	ANE69620	dpoa decarboxylase	-
A7B01_08265	1672389..1673414	-	1026	ANE69621	P-type DNA transfer ATPase VirB11	virB11
A7B01_08270	1673404..1674654	-	1251	ANE69622	type VI secretion protein	virB10
A7B01_08275	1674690..1675595	-	906	ANE73155	P-type conjugative transfer protein VirB9	virB9
A7B01_08280	1675595..1676278	-	684	ANE69623	hypothetical protein	virB8
A7B01_08285	1676500..1677573	-	1074	ANE69624	conjugal transfer protein TrbL	virB6
A7B01_08290	1677827..1678534	-	708	ANE69625	type IV secretion system protein VirB5	-
A7B01_08295	1678552..1681290	-	2739	ANE69626	ATPase	virb4
A7B01_08300	1681303..1681596	-	294	ANE69627	TriB protein	virB2
A7B01_08305	1681596..1682306	-	711	ANE69628	type VI secretion protein	virB1
A7B01_08310	1682379..1682612	-	234	ANE69629	hypothetical protein	-
A7B01_08315	1683181..1683309	-	129	ANE69630	transcriptional regulator	-
A7B01_08320	1683751..1684332	-	582	ANE69631	hypothetical protein	-
A7B01_08325	1684521..1684706	-	186	ANE69632	DNA-binding protein	-
A7B01_08330	1684846..1685772	-	927	ANE69633	hypothetical protein	-

Host bacterium

• ID: 259
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnSKGH01-1
• GenBank: CP015500
• Element size: 5490611 bp
• Coordinate of oriT [Strand]: 11944..12067 [-]
• Host bacterium: Klebsiella pneumoniae strain SKGH01
• Coordinate of element: 1658908..1717105

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -