Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200277 |
| Name | oriT_ICEKpnRJA166-1  |
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain RJA166 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP019047 (11341..11464 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnRJA166-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGACGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGACGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16798 | GenBank | ARV39379 |
| Name | t4cp2_RJA_09265_ICEKpnRJA166-1 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69479.56 Da Isoelectric Point: 6.7742
>ARV39379.1 MFS transporter [Klebsiella pneumoniae subsp. pneumoniae]
MILNKLAASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKRLIIAFSLLCFLS
GTLAPIVIGSFQWLYKTRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVITPELDKIKKHLVKKTTLEREL
RTDVRTVKSLLPQTLHYNPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSTTIRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKKIFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAERRERVTDVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIVMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTADWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRRLKTFSVS
PDIAAAAAPVRVALDFGDDDKPTALDEMTSHPALFFDENEVKPAKPQPGEEERLSPLDF
MILNKLAASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKRLIIAFSLLCFLS
GTLAPIVIGSFQWLYKTRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVITPELDKIKKHLVKKTTLEREL
RTDVRTVKSLLPQTLHYNPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSTTIRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKKIFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAERRERVTDVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIVMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTADWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRRLKTFSVS
PDIAAAAAPVRVALDFGDDDKPTALDEMTSHPALFFDENEVKPAKPQPGEEERLSPLDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16799 | GenBank | ARV39392 |
| Name | t4cp2_RJA_09335_ICEKpnRJA166-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 102964.94 Da Isoelectric Point: 7.3393
>ARV39392.1 ATPase [Klebsiella pneumoniae subsp. pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFIDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLASGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFELEVFDVVKGL
DPQSRQYVVVKNQFKRGDTKRFAARITLDLSGIGRHTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFIDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLASGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFELEVFDVVKGL
DPQSRQYVVVKNQFKRGDTKRFAARITLDLSGIGRHTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1819145..1840220
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RJA_09260 | 1814739..1815485 | - | 747 | ARV39378 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| RJA_09265 | 1815495..1817354 | - | 1860 | ARV39379 | MFS transporter | - |
| RJA_09270 | 1817351..1817632 | - | 282 | ARV39380 | hypothetical protein | - |
| RJA_09275 | 1817651..1818043 | - | 393 | Protein_1815 | hypothetical protein | - |
| RJA_09280 | 1818064..1818369 | - | 306 | ARV39381 | dpoa decarboxylase | - |
| RJA_09285 | 1818412..1818714 | - | 303 | ARV39382 | kikA from plasmid origin | - |
| RJA_09290 | 1818750..1819148 | - | 399 | ARV39383 | Cag pathogenicity island protein Cag12 | - |
| RJA_09295 | 1819145..1820170 | - | 1026 | ARV39384 | P-type DNA transfer ATPase VirB11 | virB11 |
| RJA_09300 | 1820160..1821410 | - | 1251 | ARV39385 | type VI secretion protein | virB10 |
| RJA_09305 | 1821454..1822362 | - | 909 | ARV39386 | P-type conjugative transfer protein VirB9 | virB9 |
| RJA_09310 | 1822359..1823042 | - | 684 | ARV39387 | hypothetical protein | virB8 |
| RJA_09315 | 1823072..1823191 | + | 120 | ARV39388 | hypothetical protein | - |
| RJA_09320 | 1823258..1824337 | - | 1080 | ARV39389 | conjugal transfer protein TrbL | virB6 |
| RJA_09325 | 1824349..1824576 | - | 228 | ARV39390 | hypothetical protein | - |
| RJA_09330 | 1824586..1825290 | - | 705 | ARV39391 | type IV secretion system protein VirB5 | - |
| RJA_09335 | 1825308..1828046 | - | 2739 | ARV39392 | ATPase | virb4 |
| RJA_09340 | 1828059..1828352 | - | 294 | ARV39393 | TriB protein | virB2 |
| RJA_09345 | 1828352..1828881 | - | 530 | Protein_1829 | hypothetical protein | - |
| RJA_09350 | 1828954..1829187 | - | 234 | ARV39394 | hypothetical protein | - |
| RJA_09355 | 1829672..1829857 | + | 186 | ARV39395 | transposase | - |
| RJA_09360 | 1829934..1830194 | + | 261 | ARV39396 | hypothetical protein | - |
| RJA_09365 | 1830191..1830295 | + | 105 | ARV39397 | hypothetical protein | - |
| RJA_09370 | 1830314..1830415 | + | 102 | ARV39398 | transposase | - |
| RJA_09375 | 1830495..1831148 | - | 654 | ARV39399 | SAM-dependent methyltransferase | - |
| RJA_09380 | 1831690..1832322 | + | 633 | ARV39400 | response regulator | - |
| RJA_09385 | 1832294..1832518 | + | 225 | ARV39401 | hypothetical protein | - |
| RJA_09390 | 1832698..1833249 | + | 552 | ARV39402 | hypothetical protein | - |
| RJA_09395 | 1833322..1834236 | + | 915 | ARV39403 | EamA family transporter | - |
| RJA_09400 | 1834379..1834600 | - | 222 | ARV39404 | hypothetical protein | - |
| RJA_09405 | 1835241..1836470 | - | 1230 | ARV39405 | enterochelin esterase | - |
| RJA_09410 | 1836576..1840220 | - | 3645 | ARV39406 | multidrug ABC transporter ATP-binding protein | virb4 |
| RJA_09415 | 1840358..1841473 | - | 1116 | ARV39407 | glycosyl transferase | - |
| RJA_09420 | 1841646..1841880 | - | 235 | Protein_1844 | transposase | - |
| RJA_09425 | 1841925..1842180 | - | 256 | Protein_1845 | transposase | - |
| RJA_09430 | 1842339..1844519 | + | 2181 | ARV39408 | TonB-dependent siderophore receptor | - |
| RJA_09435 | 1844674..1844919 | - | 246 | ARV39409 | hypothetical protein | - |
Host bacterium
| ID | 254 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnRJA166-1 | GenBank | CP019047 |
| Element size | 5348368 bp | Coordinate of oriT [Strand] | 11341..11464 [-] |
| Host bacterium | Klebsiella pneumoniae subsp. pneumoniae strain RJA166 | Coordinate of element | 1806260..1882407 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | rmpA, iroD, iroC, iroB, iroN, fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |