Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200276 |
| Name | oriT_ICEKpnR1701-2  |
| Organism | Klebsiella pneumoniae strain R1701 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP039968 (63945..64068 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnR1701-2
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16796 | GenBank | QCO50470 |
| Name | t4cp2_E2J61_09370_ICEKpnR1701-2 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>QCO50470.1 DUF87 domain-containing protein [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16797 | GenBank | QCO50483 |
| Name | t4cp2_E2J61_09440_ICEKpnR1701-2 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>QCO50483.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1921343..1935190
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E2J61_09340 | 1916524..1918038 | + | 1515 | QCO50465 | precolibactin peptidase ClbP | - |
| E2J61_09345 | 1918031..1918753 | + | 723 | QCO50466 | colibactin biosynthesis thioesterase ClbQ | - |
| E2J61_09350 | 1918788..1919300 | + | 513 | QCO50467 | colibactin self-protection protein ClbS | - |
| E2J61_09355 | 1919285..1919488 | + | 204 | QCO50468 | hypothetical protein | - |
| E2J61_09360 | 1920496..1920594 | - | 99 | Protein_1834 | nuclease | - |
| E2J61_09365 | 1920570..1921334 | - | 765 | QCO50469 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| E2J61_09370 | 1921343..1923082 | - | 1740 | QCO50470 | DUF87 domain-containing protein | virb4 |
| E2J61_09375 | 1923173..1923427 | - | 255 | QCO50471 | hypothetical protein | - |
| E2J61_09380 | 1923446..1923838 | - | 393 | QCO50472 | hypothetical protein | - |
| E2J61_09385 | 1923860..1924165 | - | 306 | QCO50473 | dpoa decarboxylase | - |
| E2J61_09390 | 1924208..1924510 | - | 303 | QCO50474 | kikA from plasmid origin | - |
| E2J61_09395 | 1924544..1924942 | - | 399 | QCO50475 | Cag pathogenicity island protein Cag12 | - |
| E2J61_09400 | 1924939..1925964 | - | 1026 | QCO50476 | P-type DNA transfer ATPase VirB11 | virB11 |
| E2J61_09405 | 1925954..1926274 | - | 321 | QCO50477 | hypothetical protein | - |
| E2J61_09410 | 1926286..1927530 | - | 1245 | QCO50478 | type VI secretion protein | virB10 |
| E2J61_09415 | 1927574..1928479 | - | 906 | QCO53734 | P-type conjugative transfer protein VirB9 | virB9 |
| E2J61_09420 | 1928479..1929162 | - | 684 | QCO50479 | type IV secretion system protein | virB8 |
| E2J61_09425 | 1929384..1930457 | - | 1074 | QCO50480 | type IV secretion system protein | virB6 |
| E2J61_09430 | 1930461..1930703 | - | 243 | QCO50481 | hypothetical protein | - |
| E2J61_09435 | 1930711..1931418 | - | 708 | QCO50482 | type IV secretion system protein VirB5 | - |
| E2J61_09440 | 1931436..1934174 | - | 2739 | QCO50483 | ATPase | virb4 |
| E2J61_09445 | 1934187..1934480 | - | 294 | QCO50484 | TrbC/VirB2 family protein | virB2 |
| E2J61_09450 | 1934480..1935190 | - | 711 | QCO50485 | lytic transglycosylase domain-containing protein | virB1 |
| E2J61_09455 | 1935263..1935496 | - | 234 | QCO50486 | hypothetical protein | - |
| E2J61_09460 | 1936065..1936232 | - | 168 | QCO50487 | transcriptional regulator | - |
| E2J61_09465 | 1936436..1936645 | - | 210 | QCO50488 | TraR/DksA family transcriptional regulator | - |
| E2J61_09470 | 1936635..1937216 | - | 582 | QCO50489 | DUF2857 domain-containing protein | - |
| E2J61_09475 | 1937201..1937386 | - | 186 | QCO50490 | hypothetical protein | - |
| E2J61_09480 | 1937405..1937590 | - | 186 | QCO50491 | AlpA family transcriptional regulator | - |
| E2J61_09485 | 1937730..1938284 | - | 555 | QCO53735 | hypothetical protein | - |
| E2J61_09490 | 1938300..1938713 | - | 414 | Protein_1860 | hypothetical protein | - |
Host bacterium
| ID | 253 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnR1701-2 | GenBank | CP039968 |
| Element size | 5438860 bp | Coordinate of oriT [Strand] | 63945..64068 [-] |
| Host bacterium | Klebsiella pneumoniae strain R1701 | Coordinate of element | 1859451..1969990 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | clbA, clbB, clbC, clbD, clbE, clbF, clbG, clbH, clbI, clbJ, clbK, clbL, clbM, clbN, clbO, clbP, clbQ, clbS, fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |