Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200275
• Name: oriT_ICEKpnQS17-0161-1
• Organism: Klebsiella pneumoniae strain QS17-0161
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP024458 (46133..46256 [+], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnQS17-0161-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_CR231_14430_ICEKpnQS17-0161-1

• ID: 16794
• GenBank: ATS14274
• Name: t4cp2_CR231_14430_ICEKpnQS17-0161-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103068.02 Da
• Isoelectric Point: 7.4625

>ATS14274.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

t4cp2_CR231_14495_ICEKpnQS17-0161-1

• ID: 16795
• GenBank: ATS14287
• Name: t4cp2_CR231_14495_ICEKpnQS17-0161-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69574.86 Da
• Isoelectric Point: 6.6673

>ATS14287.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (418-542)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2893200..2903135

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR231_14385	2889695..2890678	+	984	ATS14265	hypothetical protein	-
CR231_14390	2890818..2891003	+	186	ATS14266	transcriptional regulator	-
CR231_14395	2891022..2891207	+	186	ATS14267	hypothetical protein	-
CR231_14400	2891192..2891773	+	582	ATS14268	DUF2857 domain-containing protein	-
CR231_14405	2891763..2891972	+	210	ATS14269	conjugal transfer protein TraR	-
CR231_14410	2892176..2892343	+	168	ATS14270	transcriptional regulator	-
CR231_14415	2892912..2893145	+	234	ATS14271	hypothetical protein	-
CR231_14420	2893200..2893928	+	729	ATS14272	type VI secretion protein	virB1
CR231_14425	2893928..2894221	+	294	ATS14273	TriB protein	virB2
CR231_14430	2894234..2896972	+	2739	ATS14274	ATPase	virb4
CR231_14435	2896990..2897697	+	708	ATS14275	type IV secretion system protein VirB5	-
CR231_14440	2897705..2897947	+	243	ATS14276	hypothetical protein	-
CR231_14445	2897951..2899024	+	1074	ATS14277	conjugal transfer protein TrbL	virB6
CR231_14450	2899246..2899929	+	684	ATS14278	type IV secretion system protein	virB8
CR231_14455	2899926..2900834	+	909	ATS14279	P-type conjugative transfer protein VirB9	virB9
CR231_14460	2900864..2902120	+	1257	ATS14280	type VI secretion protein	virB10
CR231_14465	2902110..2903135	+	1026	ATS14281	P-type DNA transfer ATPase VirB11	virB11
CR231_14470	2903132..2903530	+	399	ATS14282	Cag pathogenicity island protein Cag12	-
CR231_14475	2903566..2903871	+	306	ATS14283	conjugal transfer protein	-
CR231_14480	2903905..2904210	+	306	ATS14284	dpoa decarboxylase	-
CR231_14485	2904231..2904623	+	393	ATS14285	hypothetical protein	-
CR231_14490	2904642..2904920	+	279	ATS14286	hypothetical protein	-
CR231_14495	2904920..2906779	+	1860	ATS14287	MFS transporter	-
CR231_14500	2906789..2907535	+	747	ATS14288	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-

Host bacterium

• ID: 252
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnQS17-0161-1
• GenBank: CP024458
• Element size: 5267491 bp
• Coordinate of oriT [Strand]: 46133..46256 [+]
• Host bacterium: Klebsiella pneumoniae strain QS17-0161
• Coordinate of element: 2858419..2916617

Cargo genes

• Drug resistance gene: -
• Virulence gene: ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -